A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines

Song et al. describe an immunoassay method, which is a multiplex, fluorescence-barcoded cell lines system that enables high-throughput screening of antibodies binding to large number of protein variants in a single-tube manner. They demonstrate the utility of this tool by assessing binding of differ...

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Autores principales:	Shengli Song, Miriam Manook, Jean Kwun, Annette M. Jackson, Stuart J. Knechtle, Garnett Kelsoe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d56c349601404cf68a744b2e7c1ca562
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spelling	oai:doaj.org-article:d56c349601404cf68a744b2e7c1ca5622021-11-28T12:10:29ZA cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines10.1038/s42003-021-02881-w2399-3642https://doaj.org/article/d56c349601404cf68a744b2e7c1ca5622021-11-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02881-whttps://doaj.org/toc/2399-3642Song et al. describe an immunoassay method, which is a multiplex, fluorescence-barcoded cell lines system that enables high-throughput screening of antibodies binding to large number of protein variants in a single-tube manner. They demonstrate the utility of this tool by assessing binding of different antibodies to a panel of influenza hemagglutinin antigens and to epitope/domain map CCR2 and CCR5 antibodies.Shengli SongMiriam ManookJean KwunAnnette M. JacksonStuart J. KnechtleGarnett KelsoeNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Shengli Song Miriam Manook Jean Kwun Annette M. Jackson Stuart J. Knechtle Garnett Kelsoe A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines
description	Song et al. describe an immunoassay method, which is a multiplex, fluorescence-barcoded cell lines system that enables high-throughput screening of antibodies binding to large number of protein variants in a single-tube manner. They demonstrate the utility of this tool by assessing binding of different antibodies to a panel of influenza hemagglutinin antigens and to epitope/domain map CCR2 and CCR5 antibodies.
format	article
author	Shengli Song Miriam Manook Jean Kwun Annette M. Jackson Stuart J. Knechtle Garnett Kelsoe
author_facet	Shengli Song Miriam Manook Jean Kwun Annette M. Jackson Stuart J. Knechtle Garnett Kelsoe
author_sort	Shengli Song
title	A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines
title_short	A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines
title_full	A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines
title_fullStr	A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines
title_full_unstemmed	A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines
title_sort	cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d56c349601404cf68a744b2e7c1ca562
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A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines

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