An Immunohistochemical Study of Cocaine- and Amphetamine-Regulated Transcript (Cart) Expression in the Pterygopalatine Ganglion of the Pig

Although, a great effort has been made to understand the synthesis, regulation, processing and function of cocaine- and amphetamine-regulated transcript (CART) peptide at the central level, its peripheral function(s) are still obscure. Moreover, scarce studies describing the presence of CART in peri...

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Autores principales:	Zacharko-Siembida Anna, Matysek Małgorzata, Szalak Radosław, Arciszewski Marcin B.
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2017
Materias:	autonomic ganglia immunohistochemistry neuropeptides pig pterygopalatine ganglion Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/d56cffaab81d4c009809559cee8752a3
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spelling	oai:doaj.org-article:d56cffaab81d4c009809559cee8752a32021-11-17T21:27:51ZAn Immunohistochemical Study of Cocaine- and Amphetamine-Regulated Transcript (Cart) Expression in the Pterygopalatine Ganglion of the Pig1820-744810.1515/acve-2017-0032https://doaj.org/article/d56cffaab81d4c009809559cee8752a32017-09-01T00:00:00Zhttps://doi.org/10.1515/acve-2017-0032https://doaj.org/toc/1820-7448Although, a great effort has been made to understand the synthesis, regulation, processing and function of cocaine- and amphetamine-regulated transcript (CART) peptide at the central level, its peripheral function(s) are still obscure. Moreover, scarce studies describing the presence of CART in peripheral autonomic ganglia are mainly limited to laboratory rodents. Thus, the aim of the present study was to immunohistochemically investigate the expression of CART in Hu C/D-positive neurons of the porcine pterygopalatine ganglion (PPG). The distribution pattern of CART-IR nerve elements in PPG has been also assessed. The co-localization of CART with substance P (SP), galanin or somatostatin was studied by means of double immunohistochemical stainings. The presence of Hu C/D-positive/CART-positive neurons was detected both in the left and right PPG (4.7±1.2% and 5.2% ± 1.4%, respectively). The CARTimmunoreactive (IR) neurons were categorized as either middle (ca. 80%) or small (ca. 20%) in size. Moderate numbers of CART-IR boutons were also detected between CART-negative ganglionic neurons. CART-IR basket-like formations around PPG neurons were regularly observed. Virtually all CART-IR neurons additionally co-stored VIP, whereas none of the CART-expressing cells showed the presence of galanin, SP or somatostatin. CART-IR basket-like formations exclusively encircled VIP-IR PPG neurons. Thus, CART-IR nerve cells seem to constitute a relatively small homologous population of the porcine PPG neurons with largely unknown functions. Further functional studies aiming at whether CART-IR neurons could serve as interneurons are necessary.Zacharko-Siembida AnnaMatysek MałgorzataSzalak RadosławArciszewski Marcin B.Sciendoarticleautonomic gangliaimmunohistochemistryneuropeptidespigpterygopalatine ganglionVeterinary medicineSF600-1100ENActa Veterinaria, Vol 67, Iss 3, Pp 397-408 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	autonomic ganglia immunohistochemistry neuropeptides pig pterygopalatine ganglion Veterinary medicine SF600-1100
spellingShingle	autonomic ganglia immunohistochemistry neuropeptides pig pterygopalatine ganglion Veterinary medicine SF600-1100 Zacharko-Siembida Anna Matysek Małgorzata Szalak Radosław Arciszewski Marcin B. An Immunohistochemical Study of Cocaine- and Amphetamine-Regulated Transcript (Cart) Expression in the Pterygopalatine Ganglion of the Pig
description	Although, a great effort has been made to understand the synthesis, regulation, processing and function of cocaine- and amphetamine-regulated transcript (CART) peptide at the central level, its peripheral function(s) are still obscure. Moreover, scarce studies describing the presence of CART in peripheral autonomic ganglia are mainly limited to laboratory rodents. Thus, the aim of the present study was to immunohistochemically investigate the expression of CART in Hu C/D-positive neurons of the porcine pterygopalatine ganglion (PPG). The distribution pattern of CART-IR nerve elements in PPG has been also assessed. The co-localization of CART with substance P (SP), galanin or somatostatin was studied by means of double immunohistochemical stainings. The presence of Hu C/D-positive/CART-positive neurons was detected both in the left and right PPG (4.7±1.2% and 5.2% ± 1.4%, respectively). The CARTimmunoreactive (IR) neurons were categorized as either middle (ca. 80%) or small (ca. 20%) in size. Moderate numbers of CART-IR boutons were also detected between CART-negative ganglionic neurons. CART-IR basket-like formations around PPG neurons were regularly observed. Virtually all CART-IR neurons additionally co-stored VIP, whereas none of the CART-expressing cells showed the presence of galanin, SP or somatostatin. CART-IR basket-like formations exclusively encircled VIP-IR PPG neurons. Thus, CART-IR nerve cells seem to constitute a relatively small homologous population of the porcine PPG neurons with largely unknown functions. Further functional studies aiming at whether CART-IR neurons could serve as interneurons are necessary.
format	article
author	Zacharko-Siembida Anna Matysek Małgorzata Szalak Radosław Arciszewski Marcin B.
author_facet	Zacharko-Siembida Anna Matysek Małgorzata Szalak Radosław Arciszewski Marcin B.
author_sort	Zacharko-Siembida Anna
title	An Immunohistochemical Study of Cocaine- and Amphetamine-Regulated Transcript (Cart) Expression in the Pterygopalatine Ganglion of the Pig
title_short	An Immunohistochemical Study of Cocaine- and Amphetamine-Regulated Transcript (Cart) Expression in the Pterygopalatine Ganglion of the Pig
title_full	An Immunohistochemical Study of Cocaine- and Amphetamine-Regulated Transcript (Cart) Expression in the Pterygopalatine Ganglion of the Pig
title_fullStr	An Immunohistochemical Study of Cocaine- and Amphetamine-Regulated Transcript (Cart) Expression in the Pterygopalatine Ganglion of the Pig
title_full_unstemmed	An Immunohistochemical Study of Cocaine- and Amphetamine-Regulated Transcript (Cart) Expression in the Pterygopalatine Ganglion of the Pig
title_sort	immunohistochemical study of cocaine- and amphetamine-regulated transcript (cart) expression in the pterygopalatine ganglion of the pig
publisher	Sciendo
publishDate	2017
url	https://doaj.org/article/d56cffaab81d4c009809559cee8752a3
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An Immunohistochemical Study of Cocaine- and Amphetamine-Regulated Transcript (Cart) Expression in the Pterygopalatine Ganglion of the Pig

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