AITRL, an evolutionarily conserved plant specific transcription repressor regulates ABA response in Arabidopsis

Abstract Expression of stress response genes can be regulated by abscisic acid (ABA) dependent and ABA independent pathways. Osmotic stresses promote ABA accumulation, therefore inducing the expression of stress response genes via ABA signaling. Whereas cold and heat stresses induce the expression o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yanxing Ma, Hainan Tian, Rao Lin, Wei Wang, Na Zhang, Saddam Hussain, Wenting Yang, Chen Zhang, Ganghua Zhou, Tianya Wang, Shucai Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d580d5f4db074545b776d646dad60d8b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d580d5f4db074545b776d646dad60d8b
record_format	dspace
spelling	oai:doaj.org-article:d580d5f4db074545b776d646dad60d8b2021-12-02T14:01:23ZAITRL, an evolutionarily conserved plant specific transcription repressor regulates ABA response in Arabidopsis10.1038/s41598-020-80695-22045-2322https://doaj.org/article/d580d5f4db074545b776d646dad60d8b2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80695-2https://doaj.org/toc/2045-2322Abstract Expression of stress response genes can be regulated by abscisic acid (ABA) dependent and ABA independent pathways. Osmotic stresses promote ABA accumulation, therefore inducing the expression of stress response genes via ABA signaling. Whereas cold and heat stresses induce the expression of stress response genes via ABA independent pathway. ABA induced transcription repressors (AITRs) are a family of novel transcription factors that play a role in ABA signaling, and Drought response gene (DRG) has previously been shown to play a role in regulating plant response to drought and freezing stresses. We report here the identification of DRG as a novel transcription factor and a regulator of ABA response in Arabidopsis. We found that the expression of DRG was induced by ABA treatment. Homologs searching identified AITR5 as the most closely related Arabidopsis protein to DRG, and homologs of DRG, including the AITR-like (AITRL) proteins in bryophytes and gymnosperms, are specifically presented in embryophytes. Therefore we renamed DRG as AITRL. Protoplast transfection assays show that AITRL functioned as a transcription repressor. In seed germination and seedling greening assays, the aitrl mutants showed an increased sensitivity to ABA. By using qRT-PCR, we show that ABA responses of some ABA signaling component genes including some PYR1-likes (PYLs), PROTEIN PHOSPHATASE 2Cs (PP2Cs) and SUCROSE NONFERMENTING 1 (SNF1)-RELATED PROTEIN KINASES 2s (SnRK2s) were reduced in the aitrl mutants. Taken together, our results suggest that AITRLs are a family of novel transcription repressors evolutionally conserved in embryophytes, and AITRL regulates ABA response in Arabidopsis by affecting ABA response of some ABA signaling component genes.Yanxing MaHainan TianRao LinWei WangNa ZhangSaddam HussainWenting YangChen ZhangGanghua ZhouTianya WangShucai WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yanxing Ma Hainan Tian Rao Lin Wei Wang Na Zhang Saddam Hussain Wenting Yang Chen Zhang Ganghua Zhou Tianya Wang Shucai Wang AITRL, an evolutionarily conserved plant specific transcription repressor regulates ABA response in Arabidopsis
description	Abstract Expression of stress response genes can be regulated by abscisic acid (ABA) dependent and ABA independent pathways. Osmotic stresses promote ABA accumulation, therefore inducing the expression of stress response genes via ABA signaling. Whereas cold and heat stresses induce the expression of stress response genes via ABA independent pathway. ABA induced transcription repressors (AITRs) are a family of novel transcription factors that play a role in ABA signaling, and Drought response gene (DRG) has previously been shown to play a role in regulating plant response to drought and freezing stresses. We report here the identification of DRG as a novel transcription factor and a regulator of ABA response in Arabidopsis. We found that the expression of DRG was induced by ABA treatment. Homologs searching identified AITR5 as the most closely related Arabidopsis protein to DRG, and homologs of DRG, including the AITR-like (AITRL) proteins in bryophytes and gymnosperms, are specifically presented in embryophytes. Therefore we renamed DRG as AITRL. Protoplast transfection assays show that AITRL functioned as a transcription repressor. In seed germination and seedling greening assays, the aitrl mutants showed an increased sensitivity to ABA. By using qRT-PCR, we show that ABA responses of some ABA signaling component genes including some PYR1-likes (PYLs), PROTEIN PHOSPHATASE 2Cs (PP2Cs) and SUCROSE NONFERMENTING 1 (SNF1)-RELATED PROTEIN KINASES 2s (SnRK2s) were reduced in the aitrl mutants. Taken together, our results suggest that AITRLs are a family of novel transcription repressors evolutionally conserved in embryophytes, and AITRL regulates ABA response in Arabidopsis by affecting ABA response of some ABA signaling component genes.
format	article
author	Yanxing Ma Hainan Tian Rao Lin Wei Wang Na Zhang Saddam Hussain Wenting Yang Chen Zhang Ganghua Zhou Tianya Wang Shucai Wang
author_facet	Yanxing Ma Hainan Tian Rao Lin Wei Wang Na Zhang Saddam Hussain Wenting Yang Chen Zhang Ganghua Zhou Tianya Wang Shucai Wang
author_sort	Yanxing Ma
title	AITRL, an evolutionarily conserved plant specific transcription repressor regulates ABA response in Arabidopsis
title_short	AITRL, an evolutionarily conserved plant specific transcription repressor regulates ABA response in Arabidopsis
title_full	AITRL, an evolutionarily conserved plant specific transcription repressor regulates ABA response in Arabidopsis
title_fullStr	AITRL, an evolutionarily conserved plant specific transcription repressor regulates ABA response in Arabidopsis
title_full_unstemmed	AITRL, an evolutionarily conserved plant specific transcription repressor regulates ABA response in Arabidopsis
title_sort	aitrl, an evolutionarily conserved plant specific transcription repressor regulates aba response in arabidopsis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d580d5f4db074545b776d646dad60d8b
work_keys_str_mv	AT yanxingma aitrlanevolutionarilyconservedplantspecifictranscriptionrepressorregulatesabaresponseinarabidopsis AT hainantian aitrlanevolutionarilyconservedplantspecifictranscriptionrepressorregulatesabaresponseinarabidopsis AT raolin aitrlanevolutionarilyconservedplantspecifictranscriptionrepressorregulatesabaresponseinarabidopsis AT weiwang aitrlanevolutionarilyconservedplantspecifictranscriptionrepressorregulatesabaresponseinarabidopsis AT nazhang aitrlanevolutionarilyconservedplantspecifictranscriptionrepressorregulatesabaresponseinarabidopsis AT saddamhussain aitrlanevolutionarilyconservedplantspecifictranscriptionrepressorregulatesabaresponseinarabidopsis AT wentingyang aitrlanevolutionarilyconservedplantspecifictranscriptionrepressorregulatesabaresponseinarabidopsis AT chenzhang aitrlanevolutionarilyconservedplantspecifictranscriptionrepressorregulatesabaresponseinarabidopsis AT ganghuazhou aitrlanevolutionarilyconservedplantspecifictranscriptionrepressorregulatesabaresponseinarabidopsis AT tianyawang aitrlanevolutionarilyconservedplantspecifictranscriptionrepressorregulatesabaresponseinarabidopsis AT shucaiwang aitrlanevolutionarilyconservedplantspecifictranscriptionrepressorregulatesabaresponseinarabidopsis
_version_	1718392172680577024

AITRL, an evolutionarily conserved plant specific transcription repressor regulates ABA response in Arabidopsis

Ejemplares similares