Photoswitchable phospholipid FRET acceptor: Detergent free intermembrane transfer assay of fluorescent lipid analogs

Abstract We have developed and characterized a novel photoswitchable phospholipid analog termed N-nitroBIPS-DPPG. The fluorescence can be switched on and off repeatedly with minimal photobleaching by UV or visible light exposure, respectively. The rather large photochromic head group is inserted dee...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mariko Sumi, Asami Makino, Takehiko Inaba, Yasushi Sako, Fumihiro Fujimori, Peter Greimel, Toshihide Kobayashi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d582f709b1b3429a9a380e5bb0e37308
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d582f709b1b3429a9a380e5bb0e37308
record_format	dspace
spelling	oai:doaj.org-article:d582f709b1b3429a9a380e5bb0e373082021-12-02T12:32:20ZPhotoswitchable phospholipid FRET acceptor: Detergent free intermembrane transfer assay of fluorescent lipid analogs10.1038/s41598-017-02980-x2045-2322https://doaj.org/article/d582f709b1b3429a9a380e5bb0e373082017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02980-xhttps://doaj.org/toc/2045-2322Abstract We have developed and characterized a novel photoswitchable phospholipid analog termed N-nitroBIPS-DPPG. The fluorescence can be switched on and off repeatedly with minimal photobleaching by UV or visible light exposure, respectively. The rather large photochromic head group is inserted deeply into the interfacial membrane region conferring a conical overall lipid shape, preference for a positive curvature and only minimal intermembrane transfer. Utilizing the switchable NBD fluorescence quenching ability of N-nitroBIPS-DPPG, a detergent free intermembrane transfer assay system for NBD modified lipids was demonstrated and validated. As NBD quenching can be turned off, total NBD associated sample fluorescence can be determined without the need of detergents. This not only reduces detergent associated systematic errors, but also simplifies assay handling and allows assay extension to detergent insoluble lipid species.Mariko SumiAsami MakinoTakehiko InabaYasushi SakoFumihiro FujimoriPeter GreimelToshihide KobayashiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariko Sumi Asami Makino Takehiko Inaba Yasushi Sako Fumihiro Fujimori Peter Greimel Toshihide Kobayashi Photoswitchable phospholipid FRET acceptor: Detergent free intermembrane transfer assay of fluorescent lipid analogs
description	Abstract We have developed and characterized a novel photoswitchable phospholipid analog termed N-nitroBIPS-DPPG. The fluorescence can be switched on and off repeatedly with minimal photobleaching by UV or visible light exposure, respectively. The rather large photochromic head group is inserted deeply into the interfacial membrane region conferring a conical overall lipid shape, preference for a positive curvature and only minimal intermembrane transfer. Utilizing the switchable NBD fluorescence quenching ability of N-nitroBIPS-DPPG, a detergent free intermembrane transfer assay system for NBD modified lipids was demonstrated and validated. As NBD quenching can be turned off, total NBD associated sample fluorescence can be determined without the need of detergents. This not only reduces detergent associated systematic errors, but also simplifies assay handling and allows assay extension to detergent insoluble lipid species.
format	article
author	Mariko Sumi Asami Makino Takehiko Inaba Yasushi Sako Fumihiro Fujimori Peter Greimel Toshihide Kobayashi
author_facet	Mariko Sumi Asami Makino Takehiko Inaba Yasushi Sako Fumihiro Fujimori Peter Greimel Toshihide Kobayashi
author_sort	Mariko Sumi
title	Photoswitchable phospholipid FRET acceptor: Detergent free intermembrane transfer assay of fluorescent lipid analogs
title_short	Photoswitchable phospholipid FRET acceptor: Detergent free intermembrane transfer assay of fluorescent lipid analogs
title_full	Photoswitchable phospholipid FRET acceptor: Detergent free intermembrane transfer assay of fluorescent lipid analogs
title_fullStr	Photoswitchable phospholipid FRET acceptor: Detergent free intermembrane transfer assay of fluorescent lipid analogs
title_full_unstemmed	Photoswitchable phospholipid FRET acceptor: Detergent free intermembrane transfer assay of fluorescent lipid analogs
title_sort	photoswitchable phospholipid fret acceptor: detergent free intermembrane transfer assay of fluorescent lipid analogs
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d582f709b1b3429a9a380e5bb0e37308
work_keys_str_mv	AT marikosumi photoswitchablephospholipidfretacceptordetergentfreeintermembranetransferassayoffluorescentlipidanalogs AT asamimakino photoswitchablephospholipidfretacceptordetergentfreeintermembranetransferassayoffluorescentlipidanalogs AT takehikoinaba photoswitchablephospholipidfretacceptordetergentfreeintermembranetransferassayoffluorescentlipidanalogs AT yasushisako photoswitchablephospholipidfretacceptordetergentfreeintermembranetransferassayoffluorescentlipidanalogs AT fumihirofujimori photoswitchablephospholipidfretacceptordetergentfreeintermembranetransferassayoffluorescentlipidanalogs AT petergreimel photoswitchablephospholipidfretacceptordetergentfreeintermembranetransferassayoffluorescentlipidanalogs AT toshihidekobayashi photoswitchablephospholipidfretacceptordetergentfreeintermembranetransferassayoffluorescentlipidanalogs
_version_	1718394123805786112

Photoswitchable phospholipid FRET acceptor: Detergent free intermembrane transfer assay of fluorescent lipid analogs

Ejemplares similares