Identification of drought tolerance markers in a diverse population of rice cultivars by expression and metabolite profiling.

Rice provides about half of the calories consumed in Asian countries, but its productivity is often reduced by drought, especially when grown under rain-fed conditions. Cultivars with increased drought tolerance have been bred over centuries. Slow selection for drought tolerance on the basis of phen...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Thomas Degenkolbe, Phuc T Do, Joachim Kopka, Ellen Zuther, Dirk K Hincha, Karin I Köhl
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d583e6258ded4224bc634718ad6ac049
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d583e6258ded4224bc634718ad6ac049
record_format	dspace
spelling	oai:doaj.org-article:d583e6258ded4224bc634718ad6ac0492021-11-18T07:44:42ZIdentification of drought tolerance markers in a diverse population of rice cultivars by expression and metabolite profiling.1932-620310.1371/journal.pone.0063637https://doaj.org/article/d583e6258ded4224bc634718ad6ac0492013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23717458/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Rice provides about half of the calories consumed in Asian countries, but its productivity is often reduced by drought, especially when grown under rain-fed conditions. Cultivars with increased drought tolerance have been bred over centuries. Slow selection for drought tolerance on the basis of phenotypic traits may be accelerated by using molecular markers identified through expression and metabolic profiling. Previously, we identified 46 candidate genes with significant genotype × environment interaction in an expression profiling study on four cultivars with contrasting drought tolerance. These potential markers and in addition GC-MS quantified metabolites were tested in 21 cultivars from both indica and japonica background that varied in drought tolerance. Leaf blades were sampled from this population of cultivars grown under control or long-term drought condition and subjected to expression analysis by qRT-PCR and metabolite profiling. Under drought stress, metabolite levels correlated mainly negatively with performance parameters, but eight metabolites correlated positively. For 28 genes, a significant correlation between expression level and performance under drought was confirmed. Negative correlations were predominant. Among those with significant positive correlation was the gene coding for a cytosolic fructose-1,6-bisphosphatase. This enzyme catalyzes a highly regulated step in C-metabolism. The metabolic and transcript marker candidates for drought tolerance were identified in a highly diverse population of cultivars. Thus, these markers may be used to select for tolerance in a wide range of rice germplasms.Thomas DegenkolbePhuc T DoJoachim KopkaEllen ZutherDirk K HinchaKarin I KöhlPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63637 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thomas Degenkolbe Phuc T Do Joachim Kopka Ellen Zuther Dirk K Hincha Karin I Köhl Identification of drought tolerance markers in a diverse population of rice cultivars by expression and metabolite profiling.
description	Rice provides about half of the calories consumed in Asian countries, but its productivity is often reduced by drought, especially when grown under rain-fed conditions. Cultivars with increased drought tolerance have been bred over centuries. Slow selection for drought tolerance on the basis of phenotypic traits may be accelerated by using molecular markers identified through expression and metabolic profiling. Previously, we identified 46 candidate genes with significant genotype × environment interaction in an expression profiling study on four cultivars with contrasting drought tolerance. These potential markers and in addition GC-MS quantified metabolites were tested in 21 cultivars from both indica and japonica background that varied in drought tolerance. Leaf blades were sampled from this population of cultivars grown under control or long-term drought condition and subjected to expression analysis by qRT-PCR and metabolite profiling. Under drought stress, metabolite levels correlated mainly negatively with performance parameters, but eight metabolites correlated positively. For 28 genes, a significant correlation between expression level and performance under drought was confirmed. Negative correlations were predominant. Among those with significant positive correlation was the gene coding for a cytosolic fructose-1,6-bisphosphatase. This enzyme catalyzes a highly regulated step in C-metabolism. The metabolic and transcript marker candidates for drought tolerance were identified in a highly diverse population of cultivars. Thus, these markers may be used to select for tolerance in a wide range of rice germplasms.
format	article
author	Thomas Degenkolbe Phuc T Do Joachim Kopka Ellen Zuther Dirk K Hincha Karin I Köhl
author_facet	Thomas Degenkolbe Phuc T Do Joachim Kopka Ellen Zuther Dirk K Hincha Karin I Köhl
author_sort	Thomas Degenkolbe
title	Identification of drought tolerance markers in a diverse population of rice cultivars by expression and metabolite profiling.
title_short	Identification of drought tolerance markers in a diverse population of rice cultivars by expression and metabolite profiling.
title_full	Identification of drought tolerance markers in a diverse population of rice cultivars by expression and metabolite profiling.
title_fullStr	Identification of drought tolerance markers in a diverse population of rice cultivars by expression and metabolite profiling.
title_full_unstemmed	Identification of drought tolerance markers in a diverse population of rice cultivars by expression and metabolite profiling.
title_sort	identification of drought tolerance markers in a diverse population of rice cultivars by expression and metabolite profiling.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d583e6258ded4224bc634718ad6ac049
work_keys_str_mv	AT thomasdegenkolbe identificationofdroughttolerancemarkersinadiversepopulationofricecultivarsbyexpressionandmetaboliteprofiling AT phuctdo identificationofdroughttolerancemarkersinadiversepopulationofricecultivarsbyexpressionandmetaboliteprofiling AT joachimkopka identificationofdroughttolerancemarkersinadiversepopulationofricecultivarsbyexpressionandmetaboliteprofiling AT ellenzuther identificationofdroughttolerancemarkersinadiversepopulationofricecultivarsbyexpressionandmetaboliteprofiling AT dirkkhincha identificationofdroughttolerancemarkersinadiversepopulationofricecultivarsbyexpressionandmetaboliteprofiling AT karinikohl identificationofdroughttolerancemarkersinadiversepopulationofricecultivarsbyexpressionandmetaboliteprofiling
_version_	1718423066019627008

Identification of drought tolerance markers in a diverse population of rice cultivars by expression and metabolite profiling.

Ejemplares similares