The antibacterial mechanism of oridonin against methicillin-resistant Staphylococcus aureus (MRSA)

Context: Methicillin-resistant Staphylococcus aureus (MRSA) is a very harmful bacterium. Oridonin, a component in Rabdosia rubescens (Hemsl.) Hara (Lamiaceae), is widely used against bacterial infections in China. Objective: We evaluated oridonin effects on MRSA cell membrane and wall, protein metab...

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Autores principales:	Zhongwei Yuan, Ping Ouyang, Kexin Gu, Tayyab Rehman, Tianyi Zhang, Zhongqiong Yin, Hualin Fu, Juchun Lin, Changliang He, Gang Shu, Xiaoxia Liang, Zhixiang Yuan, Xu Song, Lixia Li, Yuanfeng Zou, Lizi Yin
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	bacteriostat cell membrane cell wall protein metabolism ldh dna microscopic structure Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/d58c0d0bc466499ba65f48100709bc4f
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spelling	oai:doaj.org-article:d58c0d0bc466499ba65f48100709bc4f2021-11-17T14:21:56ZThe antibacterial mechanism of oridonin against methicillin-resistant Staphylococcus aureus (MRSA)1388-02091744-511610.1080/13880209.2019.1674342https://doaj.org/article/d58c0d0bc466499ba65f48100709bc4f2019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1674342https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Methicillin-resistant Staphylococcus aureus (MRSA) is a very harmful bacterium. Oridonin, a component in Rabdosia rubescens (Hemsl.) Hara (Lamiaceae), is widely used against bacterial infections in China. Objective: We evaluated oridonin effects on MRSA cell membrane and wall, protein metabolism, lactate dehydrogenase (LDH), DNA and microscopic structure. Materials and methods: Broth microdilution and flat colony counting methods were used to measure oridonin minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against USA300 strain. Electrical conductivity and DNA exosmosis were analysed to study oridonin effects (128 μg/mL) on cell membrane and wall for 0, 1, 2, 4 and 6 h. Sodium dodecyl sulphate polyacrylamide gel electrophoresis was used to detect effects on soluble protein synthesis after 6, 10 and 16 h. LDH activity was examined with an enzyme-linked immunosorbent assay. Effects of oridonin on USA300 DNA were investigated with DAPI staining. Morphological changes in MRSA following oridonin treatment were determined with scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Results: Oridonin MIC and MBC values against USA300 were 64 and 512 μg/mL, respectively. The conductivity and DNA exosmosis level of oridonin-treated USA300 improved by 3.20±0.84% and increased by 58.63 ± 1.78 μg/mL, respectively. LDH and soluble protein levels decreased by 30.85±7.69% and 27.51 ± 1.39%, respectively. A decrease in fluorescence intensity was reported with time. Oridonin affected the morphology of USA300. Conclusions: Oridonin antibacterial mechanism was related to changes in cell membrane and cell wall permeability, disturbance in protein and DNA metabolism, and influence on bacterial morphology. Thus, oridonin may help in treating MRSA infection.Zhongwei YuanPing OuyangKexin GuTayyab RehmanTianyi ZhangZhongqiong YinHualin FuJuchun LinChangliang HeGang ShuXiaoxia LiangZhixiang YuanXu SongLixia LiYuanfeng ZouLizi YinTaylor & Francis Grouparticlebacteriostatcell membranecell wallprotein metabolismldhdnamicroscopic structureTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 710-716 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteriostat cell membrane cell wall protein metabolism ldh dna microscopic structure Therapeutics. Pharmacology RM1-950
spellingShingle	bacteriostat cell membrane cell wall protein metabolism ldh dna microscopic structure Therapeutics. Pharmacology RM1-950 Zhongwei Yuan Ping Ouyang Kexin Gu Tayyab Rehman Tianyi Zhang Zhongqiong Yin Hualin Fu Juchun Lin Changliang He Gang Shu Xiaoxia Liang Zhixiang Yuan Xu Song Lixia Li Yuanfeng Zou Lizi Yin The antibacterial mechanism of oridonin against methicillin-resistant Staphylococcus aureus (MRSA)
description	Context: Methicillin-resistant Staphylococcus aureus (MRSA) is a very harmful bacterium. Oridonin, a component in Rabdosia rubescens (Hemsl.) Hara (Lamiaceae), is widely used against bacterial infections in China. Objective: We evaluated oridonin effects on MRSA cell membrane and wall, protein metabolism, lactate dehydrogenase (LDH), DNA and microscopic structure. Materials and methods: Broth microdilution and flat colony counting methods were used to measure oridonin minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against USA300 strain. Electrical conductivity and DNA exosmosis were analysed to study oridonin effects (128 μg/mL) on cell membrane and wall for 0, 1, 2, 4 and 6 h. Sodium dodecyl sulphate polyacrylamide gel electrophoresis was used to detect effects on soluble protein synthesis after 6, 10 and 16 h. LDH activity was examined with an enzyme-linked immunosorbent assay. Effects of oridonin on USA300 DNA were investigated with DAPI staining. Morphological changes in MRSA following oridonin treatment were determined with scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Results: Oridonin MIC and MBC values against USA300 were 64 and 512 μg/mL, respectively. The conductivity and DNA exosmosis level of oridonin-treated USA300 improved by 3.20±0.84% and increased by 58.63 ± 1.78 μg/mL, respectively. LDH and soluble protein levels decreased by 30.85±7.69% and 27.51 ± 1.39%, respectively. A decrease in fluorescence intensity was reported with time. Oridonin affected the morphology of USA300. Conclusions: Oridonin antibacterial mechanism was related to changes in cell membrane and cell wall permeability, disturbance in protein and DNA metabolism, and influence on bacterial morphology. Thus, oridonin may help in treating MRSA infection.
format	article
author	Zhongwei Yuan Ping Ouyang Kexin Gu Tayyab Rehman Tianyi Zhang Zhongqiong Yin Hualin Fu Juchun Lin Changliang He Gang Shu Xiaoxia Liang Zhixiang Yuan Xu Song Lixia Li Yuanfeng Zou Lizi Yin
author_facet	Zhongwei Yuan Ping Ouyang Kexin Gu Tayyab Rehman Tianyi Zhang Zhongqiong Yin Hualin Fu Juchun Lin Changliang He Gang Shu Xiaoxia Liang Zhixiang Yuan Xu Song Lixia Li Yuanfeng Zou Lizi Yin
author_sort	Zhongwei Yuan
title	The antibacterial mechanism of oridonin against methicillin-resistant Staphylococcus aureus (MRSA)
title_short	The antibacterial mechanism of oridonin against methicillin-resistant Staphylococcus aureus (MRSA)
title_full	The antibacterial mechanism of oridonin against methicillin-resistant Staphylococcus aureus (MRSA)
title_fullStr	The antibacterial mechanism of oridonin against methicillin-resistant Staphylococcus aureus (MRSA)
title_full_unstemmed	The antibacterial mechanism of oridonin against methicillin-resistant Staphylococcus aureus (MRSA)
title_sort	antibacterial mechanism of oridonin against methicillin-resistant staphylococcus aureus (mrsa)
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/d58c0d0bc466499ba65f48100709bc4f
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The antibacterial mechanism of oridonin against methicillin-resistant Staphylococcus aureus (MRSA)

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