EXPRESSION OF MATRIX METALLOPROTEINASES IN LACRIMAL FLUID AND GENE POLYMORPHISM OF COMPLEMENT FACTOR H (CFH) IN PATIENTS WITH PRIMARY OPEN-ANGLE GLAUCOMA

The objective of our study was to evaluate production of matrix metalloproteinase–2 and -9 in lacrimal fluid, as well as СFH gene polymorphism in a group of patients with primary open-angle glaucoma. The study was performed with 120 eyes of patients with primary open-angle glaucoma (POAG) includin...

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Autores principales:	V. A. Sokolov, V. G. Likhvantseva, O. N. Levanova, A. A. Nikiforov, V. A. Vygodin
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2017
Materias:	primary open-angle glaucoma complement factor h gene cfh polymorphism matrix metalloproteinases Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/d591654485734c81a12f297e4aff09ca
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spelling	oai:doaj.org-article:d591654485734c81a12f297e4aff09ca2021-11-18T08:03:46ZEXPRESSION OF MATRIX METALLOPROTEINASES IN LACRIMAL FLUID AND GENE POLYMORPHISM OF COMPLEMENT FACTOR H (CFH) IN PATIENTS WITH PRIMARY OPEN-ANGLE GLAUCOMA1563-06252313-741X10.15789/1563-0625-2017-5-547-556https://doaj.org/article/d591654485734c81a12f297e4aff09ca2017-10-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1354https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe objective of our study was to evaluate production of matrix metalloproteinase–2 and -9 in lacrimal fluid, as well as СFH gene polymorphism in a group of patients with primary open-angle glaucoma. The study was performed with 120 eyes of patients with primary open-angle glaucoma (POAG) including 34 eyes from the first-stage disease; 35 eyes, from the 2nd stage; 35, at the 3rd stage of the disorder, and 16 eyes with stage 4 POAG. Control group consisted of 25 glaucoma-free patients. Tear fluid served as a material for biochemical tests. The levels of MMP-2 and MMP-9 were determined by sandwich ELISA for the both eyes. Venous blood was used for genetic studies. Genomic human DNA was isolated from the whole blood leukocytes, a polymerase chain reaction (PCR) was performed. The results of this study were analyzed using a package of SAS applied statistical programs (Statistical Analysis System, SAS Institute Inc., USA) employing standard algorithms of variation statistics, i.e., correlation analysis, contingency tables, and various types of intergroup comparisons for the parameters studied. The following results were obtained: average indices for metalloproteinases-2 and -9 were found to be increased upon progression of the disease. Correlation analysis of contingency relationships was performed for morphometric, clinical, functional parameters, and quantitative indices of MMP-2 and MMP-9. We also studied prevalence of CFH gene polymorphismic alleles depending on the stage of glaucoma development. One may conclude that our study provided evidence for a relationship between MMP-2 and MMP-9 production, and СFH gene polymorphism, thus expanding a list of host genetic mutations which could be potentially involved into pathogenesis of primary open-angle glaucoma.V. A. SokolovV. G. LikhvantsevaO. N. LevanovaA. A. NikiforovV. A. VygodinSPb RAACIarticleprimary open-angle glaucomacomplement factor hgene cfhpolymorphismmatrix metalloproteinasesImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 19, Iss 5, Pp 547-556 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	primary open-angle glaucoma complement factor h gene cfh polymorphism matrix metalloproteinases Immunologic diseases. Allergy RC581-607
spellingShingle	primary open-angle glaucoma complement factor h gene cfh polymorphism matrix metalloproteinases Immunologic diseases. Allergy RC581-607 V. A. Sokolov V. G. Likhvantseva O. N. Levanova A. A. Nikiforov V. A. Vygodin EXPRESSION OF MATRIX METALLOPROTEINASES IN LACRIMAL FLUID AND GENE POLYMORPHISM OF COMPLEMENT FACTOR H (CFH) IN PATIENTS WITH PRIMARY OPEN-ANGLE GLAUCOMA
description	The objective of our study was to evaluate production of matrix metalloproteinase–2 and -9 in lacrimal fluid, as well as СFH gene polymorphism in a group of patients with primary open-angle glaucoma. The study was performed with 120 eyes of patients with primary open-angle glaucoma (POAG) including 34 eyes from the first-stage disease; 35 eyes, from the 2nd stage; 35, at the 3rd stage of the disorder, and 16 eyes with stage 4 POAG. Control group consisted of 25 glaucoma-free patients. Tear fluid served as a material for biochemical tests. The levels of MMP-2 and MMP-9 were determined by sandwich ELISA for the both eyes. Venous blood was used for genetic studies. Genomic human DNA was isolated from the whole blood leukocytes, a polymerase chain reaction (PCR) was performed. The results of this study were analyzed using a package of SAS applied statistical programs (Statistical Analysis System, SAS Institute Inc., USA) employing standard algorithms of variation statistics, i.e., correlation analysis, contingency tables, and various types of intergroup comparisons for the parameters studied. The following results were obtained: average indices for metalloproteinases-2 and -9 were found to be increased upon progression of the disease. Correlation analysis of contingency relationships was performed for morphometric, clinical, functional parameters, and quantitative indices of MMP-2 and MMP-9. We also studied prevalence of CFH gene polymorphismic alleles depending on the stage of glaucoma development. One may conclude that our study provided evidence for a relationship between MMP-2 and MMP-9 production, and СFH gene polymorphism, thus expanding a list of host genetic mutations which could be potentially involved into pathogenesis of primary open-angle glaucoma.
format	article
author	V. A. Sokolov V. G. Likhvantseva O. N. Levanova A. A. Nikiforov V. A. Vygodin
author_facet	V. A. Sokolov V. G. Likhvantseva O. N. Levanova A. A. Nikiforov V. A. Vygodin
author_sort	V. A. Sokolov
title	EXPRESSION OF MATRIX METALLOPROTEINASES IN LACRIMAL FLUID AND GENE POLYMORPHISM OF COMPLEMENT FACTOR H (CFH) IN PATIENTS WITH PRIMARY OPEN-ANGLE GLAUCOMA
title_short	EXPRESSION OF MATRIX METALLOPROTEINASES IN LACRIMAL FLUID AND GENE POLYMORPHISM OF COMPLEMENT FACTOR H (CFH) IN PATIENTS WITH PRIMARY OPEN-ANGLE GLAUCOMA
title_full	EXPRESSION OF MATRIX METALLOPROTEINASES IN LACRIMAL FLUID AND GENE POLYMORPHISM OF COMPLEMENT FACTOR H (CFH) IN PATIENTS WITH PRIMARY OPEN-ANGLE GLAUCOMA
title_fullStr	EXPRESSION OF MATRIX METALLOPROTEINASES IN LACRIMAL FLUID AND GENE POLYMORPHISM OF COMPLEMENT FACTOR H (CFH) IN PATIENTS WITH PRIMARY OPEN-ANGLE GLAUCOMA
title_full_unstemmed	EXPRESSION OF MATRIX METALLOPROTEINASES IN LACRIMAL FLUID AND GENE POLYMORPHISM OF COMPLEMENT FACTOR H (CFH) IN PATIENTS WITH PRIMARY OPEN-ANGLE GLAUCOMA
title_sort	expression of matrix metalloproteinases in lacrimal fluid and gene polymorphism of complement factor h (cfh) in patients with primary open-angle glaucoma
publisher	SPb RAACI
publishDate	2017
url	https://doaj.org/article/d591654485734c81a12f297e4aff09ca
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_version_	1718422383708078080

EXPRESSION OF MATRIX METALLOPROTEINASES IN LACRIMAL FLUID AND GENE POLYMORPHISM OF COMPLEMENT FACTOR H (CFH) IN PATIENTS WITH PRIMARY OPEN-ANGLE GLAUCOMA

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