Therapeutic effects of Sheng Xue Fang in a cyclophosphamide-induced anaemia mouse model

Context Sheng Xue Fang (SXF) has been used to treat anaemia for decades with good efficacy. Objective To study the effect and possible mechanism of SXF to restore haematopoietic function. Materials and methods Balb/c mice (10 per/group, half male, half female) were treated with SXF (three dose group...

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Autores principales:	Lu Dou, Xue Gong, Qing Wu, Fangzheng Mou
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	network pharmacology red blood cell haematopoiesis Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/d597c885cb164c69a641891a9b5b522c
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spelling	oai:doaj.org-article:d597c885cb164c69a641891a9b5b522c2021-11-04T15:00:41ZTherapeutic effects of Sheng Xue Fang in a cyclophosphamide-induced anaemia mouse model1388-02091744-511610.1080/13880209.2021.1941133https://doaj.org/article/d597c885cb164c69a641891a9b5b522c2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2021.1941133https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context Sheng Xue Fang (SXF) has been used to treat anaemia for decades with good efficacy. Objective To study the effect and possible mechanism of SXF to restore haematopoietic function. Materials and methods Balb/c mice (10 per/group, half male, half female) were treated with SXF (three dose groups, 8.5, 17, and 22.1 g/kg) by gavage for 14 days, and cyclophosphamide (80 mg/kg) was injected on days 10–12. Only injection of cyclophosphamide (negative control) or physiological saline (blank control) were included as controls. The spleen and femur were processed for histopathology. Active components and the target of SXF were screened. The target was used for gene enrichment and protein-protein interaction (PPI) analysis. Results Red blood cell relative changes in the SXF group (low: −5.50 ± 1.58%; medium: −11.11 ± 4.15%; high: −8.81 ± 2.67%) and relative negative control (26.21 ± 2.51%) significantly increased (all p < 0.01) in female mice. Haemoglobin and red blood cell-specific volume showed the same trend. However, SXF did not have significant effects on male mice. Splenic index in the medium group (4.44 ± 0.46%) relative negative control (3.38 ± 0.10%) significantly improved (p < 0.01) in female mice. Using network pharmacology, 77 active components and 337 targets were screened from SXF. These targets are closely related to the mitogen-activated protein kinase pathway. Conclusions SXF has good clinical application potential. However, the mechanism requires in-depth research. Our findings are of great significance in anaemia treatment and provide a new perspective for Chinese medicine research.Lu DouXue GongQing WuFangzheng MouTaylor & Francis Grouparticlenetwork pharmacologyred blood cellhaematopoiesisTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 59, Iss 1, Pp 789-798 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	network pharmacology red blood cell haematopoiesis Therapeutics. Pharmacology RM1-950
spellingShingle	network pharmacology red blood cell haematopoiesis Therapeutics. Pharmacology RM1-950 Lu Dou Xue Gong Qing Wu Fangzheng Mou Therapeutic effects of Sheng Xue Fang in a cyclophosphamide-induced anaemia mouse model
description	Context Sheng Xue Fang (SXF) has been used to treat anaemia for decades with good efficacy. Objective To study the effect and possible mechanism of SXF to restore haematopoietic function. Materials and methods Balb/c mice (10 per/group, half male, half female) were treated with SXF (three dose groups, 8.5, 17, and 22.1 g/kg) by gavage for 14 days, and cyclophosphamide (80 mg/kg) was injected on days 10–12. Only injection of cyclophosphamide (negative control) or physiological saline (blank control) were included as controls. The spleen and femur were processed for histopathology. Active components and the target of SXF were screened. The target was used for gene enrichment and protein-protein interaction (PPI) analysis. Results Red blood cell relative changes in the SXF group (low: −5.50 ± 1.58%; medium: −11.11 ± 4.15%; high: −8.81 ± 2.67%) and relative negative control (26.21 ± 2.51%) significantly increased (all p < 0.01) in female mice. Haemoglobin and red blood cell-specific volume showed the same trend. However, SXF did not have significant effects on male mice. Splenic index in the medium group (4.44 ± 0.46%) relative negative control (3.38 ± 0.10%) significantly improved (p < 0.01) in female mice. Using network pharmacology, 77 active components and 337 targets were screened from SXF. These targets are closely related to the mitogen-activated protein kinase pathway. Conclusions SXF has good clinical application potential. However, the mechanism requires in-depth research. Our findings are of great significance in anaemia treatment and provide a new perspective for Chinese medicine research.
format	article
author	Lu Dou Xue Gong Qing Wu Fangzheng Mou
author_facet	Lu Dou Xue Gong Qing Wu Fangzheng Mou
author_sort	Lu Dou
title	Therapeutic effects of Sheng Xue Fang in a cyclophosphamide-induced anaemia mouse model
title_short	Therapeutic effects of Sheng Xue Fang in a cyclophosphamide-induced anaemia mouse model
title_full	Therapeutic effects of Sheng Xue Fang in a cyclophosphamide-induced anaemia mouse model
title_fullStr	Therapeutic effects of Sheng Xue Fang in a cyclophosphamide-induced anaemia mouse model
title_full_unstemmed	Therapeutic effects of Sheng Xue Fang in a cyclophosphamide-induced anaemia mouse model
title_sort	therapeutic effects of sheng xue fang in a cyclophosphamide-induced anaemia mouse model
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/d597c885cb164c69a641891a9b5b522c
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Therapeutic effects of Sheng Xue Fang in a cyclophosphamide-induced anaemia mouse model

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