Synergistic interactions between antimicrobial peptides derived from plectasin and lipid nanocapsules containing monolaurin as a cosurfactant against Staphylococcus aureus

Anita Umerska,1 Viviane Cassisa,2 Guillaume Bastiat,1 Nada Matougui,1 Hassan Nehme,1 Florence Manero,3 Matthieu Eveillard,4 Patrick Saulnier1 1MINT, UNIV Angers, INSERM 1066, CNRS 6021, Université Bretagne Loire, Angers, Cedex, France; 2Laboratoire de bactériologie, CHU Angers...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Umerska A, Cassisa V, Bastiat G, Matougui N, Nehme H, Manero F, Eveillard M, Saulnier P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	lipid nanocapsules nanoparticles antimicrobial peptides plectasin glycerol monolaurate monolaurin Staphylococcus aureus synergy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d59877e295b3460d88d197299da197a8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d59877e295b3460d88d197299da197a8
record_format	dspace
spelling	oai:doaj.org-article:d59877e295b3460d88d197299da197a82021-12-02T07:13:44ZSynergistic interactions between antimicrobial peptides derived from plectasin and lipid nanocapsules containing monolaurin as a cosurfactant against Staphylococcus aureus1178-2013https://doaj.org/article/d59877e295b3460d88d197299da197a82017-08-01T00:00:00Zhttps://www.dovepress.com/synergistic-interactions-between-antimicrobial-peptides-derived-from-p-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Anita Umerska,1 Viviane Cassisa,2 Guillaume Bastiat,1 Nada Matougui,1 Hassan Nehme,1 Florence Manero,3 Matthieu Eveillard,4 Patrick Saulnier1 1MINT, UNIV Angers, INSERM 1066, CNRS 6021, Université Bretagne Loire, Angers, Cedex, France; 2Laboratoire de bactériologie, CHU Angers, France; 3SCIAM (Service Commun d’Imagerie et d’Analyses Microscopiques), Angers, France; 4Equipe ATIP AVENIR, CRCINA, Inserm, Université de Nantes, Université d’Angers, Angers, France Abstract: Development of effective antibacterial agents for the treatment of infections caused by Gram-positive bacteria resistant to existing antibiotics, such as methicillin-resistant Staphylococcus aureus (MRSA), is an area of intensive research. In this work, the antibacterial efficacy of two antimicrobial peptides derived from plectasin, AP114 and AP138, used alone and in combination with monolaurin-lipid nanocapsules (ML-LNCs) was evaluated. Several interesting findings emerged from the present study. First, ML-LNCs and both plectasin derivatives showed potent activity against all 14 tested strains of S. aureus, independent of their resistance phenotype. Both peptides displayed a considerable adsorption (33%–62%) onto ML-LNCs without having an important impact on the particle properties such as size. The combinations of peptide with ML-LNC displayed synergistic effect against S. aureus, as confirmed by two methods: checkerboard and time-kill assays. This synergistic interaction enables a dose reduction and consequently decreases the risk of toxicity and has the potential of minimizing the development of resistance. Together, these results suggest that ML-LNCs loaded with a plectasin derivative may be a very promising drug delivery system for further development as a novel antibacterial agent against S. aureus, including MRSA. Keywords: nanoparticles, antimicrobial peptides, glycerol monolaurate, synergy, antibiotic resistance, MRSA, methicillin-resistant Staphylococcus aureusUmerska ACassisa VBastiat GMatougui NNehme HManero FEveillard MSaulnier PDove Medical Pressarticlelipid nanocapsulesnanoparticlesantimicrobial peptidesplectasinglycerol monolauratemonolaurinStaphylococcus aureussynergyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 5687-5699 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	lipid nanocapsules nanoparticles antimicrobial peptides plectasin glycerol monolaurate monolaurin Staphylococcus aureus synergy Medicine (General) R5-920
spellingShingle	lipid nanocapsules nanoparticles antimicrobial peptides plectasin glycerol monolaurate monolaurin Staphylococcus aureus synergy Medicine (General) R5-920 Umerska A Cassisa V Bastiat G Matougui N Nehme H Manero F Eveillard M Saulnier P Synergistic interactions between antimicrobial peptides derived from plectasin and lipid nanocapsules containing monolaurin as a cosurfactant against Staphylococcus aureus
description	Anita Umerska,1 Viviane Cassisa,2 Guillaume Bastiat,1 Nada Matougui,1 Hassan Nehme,1 Florence Manero,3 Matthieu Eveillard,4 Patrick Saulnier1 1MINT, UNIV Angers, INSERM 1066, CNRS 6021, Université Bretagne Loire, Angers, Cedex, France; 2Laboratoire de bactériologie, CHU Angers, France; 3SCIAM (Service Commun d’Imagerie et d’Analyses Microscopiques), Angers, France; 4Equipe ATIP AVENIR, CRCINA, Inserm, Université de Nantes, Université d’Angers, Angers, France Abstract: Development of effective antibacterial agents for the treatment of infections caused by Gram-positive bacteria resistant to existing antibiotics, such as methicillin-resistant Staphylococcus aureus (MRSA), is an area of intensive research. In this work, the antibacterial efficacy of two antimicrobial peptides derived from plectasin, AP114 and AP138, used alone and in combination with monolaurin-lipid nanocapsules (ML-LNCs) was evaluated. Several interesting findings emerged from the present study. First, ML-LNCs and both plectasin derivatives showed potent activity against all 14 tested strains of S. aureus, independent of their resistance phenotype. Both peptides displayed a considerable adsorption (33%–62%) onto ML-LNCs without having an important impact on the particle properties such as size. The combinations of peptide with ML-LNC displayed synergistic effect against S. aureus, as confirmed by two methods: checkerboard and time-kill assays. This synergistic interaction enables a dose reduction and consequently decreases the risk of toxicity and has the potential of minimizing the development of resistance. Together, these results suggest that ML-LNCs loaded with a plectasin derivative may be a very promising drug delivery system for further development as a novel antibacterial agent against S. aureus, including MRSA. Keywords: nanoparticles, antimicrobial peptides, glycerol monolaurate, synergy, antibiotic resistance, MRSA, methicillin-resistant Staphylococcus aureus
format	article
author	Umerska A Cassisa V Bastiat G Matougui N Nehme H Manero F Eveillard M Saulnier P
author_facet	Umerska A Cassisa V Bastiat G Matougui N Nehme H Manero F Eveillard M Saulnier P
author_sort	Umerska A
title	Synergistic interactions between antimicrobial peptides derived from plectasin and lipid nanocapsules containing monolaurin as a cosurfactant against Staphylococcus aureus
title_short	Synergistic interactions between antimicrobial peptides derived from plectasin and lipid nanocapsules containing monolaurin as a cosurfactant against Staphylococcus aureus
title_full	Synergistic interactions between antimicrobial peptides derived from plectasin and lipid nanocapsules containing monolaurin as a cosurfactant against Staphylococcus aureus
title_fullStr	Synergistic interactions between antimicrobial peptides derived from plectasin and lipid nanocapsules containing monolaurin as a cosurfactant against Staphylococcus aureus
title_full_unstemmed	Synergistic interactions between antimicrobial peptides derived from plectasin and lipid nanocapsules containing monolaurin as a cosurfactant against Staphylococcus aureus
title_sort	synergistic interactions between antimicrobial peptides derived from plectasin and lipid nanocapsules containing monolaurin as a cosurfactant against staphylococcus aureus
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/d59877e295b3460d88d197299da197a8
work_keys_str_mv	AT umerskaa synergisticinteractionsbetweenantimicrobialpeptidesderivedfromplectasinandlipidnanocapsulescontainingmonolaurinasacosurfactantagainststaphylococcusaureus AT cassisav synergisticinteractionsbetweenantimicrobialpeptidesderivedfromplectasinandlipidnanocapsulescontainingmonolaurinasacosurfactantagainststaphylococcusaureus AT bastiatg synergisticinteractionsbetweenantimicrobialpeptidesderivedfromplectasinandlipidnanocapsulescontainingmonolaurinasacosurfactantagainststaphylococcusaureus AT matouguin synergisticinteractionsbetweenantimicrobialpeptidesderivedfromplectasinandlipidnanocapsulescontainingmonolaurinasacosurfactantagainststaphylococcusaureus AT nehmeh synergisticinteractionsbetweenantimicrobialpeptidesderivedfromplectasinandlipidnanocapsulescontainingmonolaurinasacosurfactantagainststaphylococcusaureus AT manerof synergisticinteractionsbetweenantimicrobialpeptidesderivedfromplectasinandlipidnanocapsulescontainingmonolaurinasacosurfactantagainststaphylococcusaureus AT eveillardm synergisticinteractionsbetweenantimicrobialpeptidesderivedfromplectasinandlipidnanocapsulescontainingmonolaurinasacosurfactantagainststaphylococcusaureus AT saulnierp synergisticinteractionsbetweenantimicrobialpeptidesderivedfromplectasinandlipidnanocapsulescontainingmonolaurinasacosurfactantagainststaphylococcusaureus
_version_	1718399546131742720

Synergistic interactions between antimicrobial peptides derived from plectasin and lipid nanocapsules containing monolaurin as a cosurfactant against Staphylococcus aureus

Ejemplares similares