Investigating nanostructured liquid crystalline particles as prospective ocular delivery vehicle for tobramycin sulfate: Ex vivo and in vivo studies

Tobramycin remains the anchor drug for bacterial keratitis treatment and management; however, unlike other aminoglycosides, it does not pass through the gastrointestinal tract. The aim of the current investigation was to formulate tobramycin-loaded nanostructured liquid crystalline particles as an o...

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Autores principales:	Shreya Kaul, Upendra Nagaich, Navneet Verma
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2021
Materias:	bacterial keratitis cubosomes hen's egg chorioallantoic membrane test nanostructured liquid crystalline particles ocular pharmacokinetic studies Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/d5998b309bbd47e689e055786fe8ed0d
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spelling	oai:doaj.org-article:d5998b309bbd47e689e055786fe8ed0d2021-11-12T10:17:53ZInvestigating nanostructured liquid crystalline particles as prospective ocular delivery vehicle for tobramycin sulfate: Ex vivo and in vivo studies2231-40400976-209410.4103/japtr.japtr_188_21https://doaj.org/article/d5998b309bbd47e689e055786fe8ed0d2021-01-01T00:00:00Zhttp://www.japtr.org/article.asp?issn=2231-4040;year=2021;volume=12;issue=4;spage=356;epage=361;aulast=Kaulhttps://doaj.org/toc/2231-4040https://doaj.org/toc/0976-2094Tobramycin remains the anchor drug for bacterial keratitis treatment and management; however, unlike other aminoglycosides, it does not pass through the gastrointestinal tract. The aim of the current investigation was to formulate tobramycin-loaded nanostructured liquid crystalline particles as an ophthalmic drug delivery system to ameliorate its preocular residence duration and ophthalmic bioavailability. Tobramycin cubosomes were fabricated by liquid–lipid monoolein, water, and poloxamer 407 as a stabilizer. Corneal penetration studies exhibited that the apparent permeation coefficient of tobramycin cubosomes was nearly 3.6-fold greater than marketed tobramycin eye drops. Ocular in vivo analysis performed in rabbits' eyes manifested that the intensity of bacterial keratitis was reduced on day 3, and on day 5, the manifestations were considerably mitigated with tobramycin cubosomes as compared to marked eye drops. Pharmacokinetic study of rabbit aqueous humor demonstrated that the area under curve and the peak concentration of optimized cubosomes were 3.1-fold and 3.3-fold, respectively, which was significantly higher than marketed eye drops. Moreover, histopathological studies illustrated the existence of normal ocular structures, thus indicating that there was no damage to the corneal epithelium or stromal layer. Consequently, the results acquired demonstrated that tobramycin-loaded cubosomal formulation could be a propitious lipid-based nanodelivery system that would enhance retention time and corneal permeability contrast to commercial eye drops.Shreya KaulUpendra NagaichNavneet VermaWolters Kluwer Medknow Publicationsarticlebacterial keratitiscubosomeshen's egg chorioallantoic membrane testnanostructured liquid crystalline particlesocular pharmacokinetic studiesTherapeutics. PharmacologyRM1-950Pharmacy and materia medicaRS1-441ENJournal of Advanced Pharmaceutical Technology & Research, Vol 12, Iss 4, Pp 356-361 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacterial keratitis cubosomes hen's egg chorioallantoic membrane test nanostructured liquid crystalline particles ocular pharmacokinetic studies Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441
spellingShingle	bacterial keratitis cubosomes hen's egg chorioallantoic membrane test nanostructured liquid crystalline particles ocular pharmacokinetic studies Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441 Shreya Kaul Upendra Nagaich Navneet Verma Investigating nanostructured liquid crystalline particles as prospective ocular delivery vehicle for tobramycin sulfate: Ex vivo and in vivo studies
description	Tobramycin remains the anchor drug for bacterial keratitis treatment and management; however, unlike other aminoglycosides, it does not pass through the gastrointestinal tract. The aim of the current investigation was to formulate tobramycin-loaded nanostructured liquid crystalline particles as an ophthalmic drug delivery system to ameliorate its preocular residence duration and ophthalmic bioavailability. Tobramycin cubosomes were fabricated by liquid–lipid monoolein, water, and poloxamer 407 as a stabilizer. Corneal penetration studies exhibited that the apparent permeation coefficient of tobramycin cubosomes was nearly 3.6-fold greater than marketed tobramycin eye drops. Ocular in vivo analysis performed in rabbits' eyes manifested that the intensity of bacterial keratitis was reduced on day 3, and on day 5, the manifestations were considerably mitigated with tobramycin cubosomes as compared to marked eye drops. Pharmacokinetic study of rabbit aqueous humor demonstrated that the area under curve and the peak concentration of optimized cubosomes were 3.1-fold and 3.3-fold, respectively, which was significantly higher than marketed eye drops. Moreover, histopathological studies illustrated the existence of normal ocular structures, thus indicating that there was no damage to the corneal epithelium or stromal layer. Consequently, the results acquired demonstrated that tobramycin-loaded cubosomal formulation could be a propitious lipid-based nanodelivery system that would enhance retention time and corneal permeability contrast to commercial eye drops.
format	article
author	Shreya Kaul Upendra Nagaich Navneet Verma
author_facet	Shreya Kaul Upendra Nagaich Navneet Verma
author_sort	Shreya Kaul
title	Investigating nanostructured liquid crystalline particles as prospective ocular delivery vehicle for tobramycin sulfate: Ex vivo and in vivo studies
title_short	Investigating nanostructured liquid crystalline particles as prospective ocular delivery vehicle for tobramycin sulfate: Ex vivo and in vivo studies
title_full	Investigating nanostructured liquid crystalline particles as prospective ocular delivery vehicle for tobramycin sulfate: Ex vivo and in vivo studies
title_fullStr	Investigating nanostructured liquid crystalline particles as prospective ocular delivery vehicle for tobramycin sulfate: Ex vivo and in vivo studies
title_full_unstemmed	Investigating nanostructured liquid crystalline particles as prospective ocular delivery vehicle for tobramycin sulfate: Ex vivo and in vivo studies
title_sort	investigating nanostructured liquid crystalline particles as prospective ocular delivery vehicle for tobramycin sulfate: ex vivo and in vivo studies
publisher	Wolters Kluwer Medknow Publications
publishDate	2021
url	https://doaj.org/article/d5998b309bbd47e689e055786fe8ed0d
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Investigating nanostructured liquid crystalline particles as prospective ocular delivery vehicle for tobramycin sulfate: Ex vivo and in vivo studies

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