Analysis of CCND1 protein and circulatory antioxidant enzyme activity association in oral squamous cell carcinoma

Antioxidants are involved in the process of cellular damage prevention, which is considered as an avenue for cancer development. Free radicals are produced in the body upon exposure to stress, cigarette smoke, alcohol, toxins found in personal care products, pesticides in foods, radiation from the s...

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Autores principales:	P.S. Sushma, Kaiser Jamil, P. Udaykumar, Fahad M. Aldakheel, Shatha A. Alduraywish, Bader Homoud Alali, Ayesha Mateen, Rabbani Syed
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Antioxidants CCND1 Protein Smoking OSCC Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d59b641c41054c15bb9043933d332d67
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spelling	oai:doaj.org-article:d59b641c41054c15bb9043933d332d672021-11-20T04:56:39ZAnalysis of CCND1 protein and circulatory antioxidant enzyme activity association in oral squamous cell carcinoma1319-562X10.1016/j.sjbs.2021.07.085https://doaj.org/article/d59b641c41054c15bb9043933d332d672021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21006689https://doaj.org/toc/1319-562XAntioxidants are involved in the process of cellular damage prevention, which is considered as an avenue for cancer development. Free radicals are produced in the body upon exposure to stress, cigarette smoke, alcohol, toxins found in personal care products, pesticides in foods, radiation from the sun, viruses, germs or fungi etc. CCND1/CyclinD1 protein was found to be overexpressed in Oral squamous cell carcinoma. One hundred patients with oral squamous cell carcinoma were recruited along with hundred controls for this study from MNJ institute of Oncology with the approval of Ethics Committee, 5 ml blood samples were collected from each patient and centrifuged to collect serum for various assays. The antioxidant enzymes like catalase, SOD, GPX and GST were estimated using enzymatic assays. Results were expressed as unit of activity for mg of protein. Insilco analysis is performed using STRING v 11 Protein interaction tool. The patients with oral cancer had significantly reduced activities of SOD, GST and GPX (1.49 ± 0.49, 3.97 ± 0.86 and 10.7 ± 0.73 respectively) compared to healthy controls (4.37 ± 1.43, 6.10 ± 1.12 and 13.8 ± 1.25 respectively) (p < 0.005). However no significant difference was observed with regard to catalase activity (2.71 ± 6.51 and 4.03 ± 1.48) (p = 0.28). The proteins interaction PPI enrichment p-value was found to be 3.22e-10 predicted significantly more interactions. Our research findings shown that there was a decline in activity of superoxide dismutase, glutathione peroxidase and glutathione s transferase in addition, personal habits like smoking play a major role in the development and progression of oral carcinogenesis and based on Insilco analysis results CCND1/Cyclin D1 could be the potential therapeutic target in oral squamous cell carcinoma.P.S. SushmaKaiser JamilP. UdaykumarFahad M. AldakheelShatha A. AlduraywishBader Homoud AlaliAyesha MateenRabbani SyedElsevierarticleAntioxidantsCCND1 ProteinSmokingOSCCBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 6987-6991 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Antioxidants CCND1 Protein Smoking OSCC Biology (General) QH301-705.5
spellingShingle	Antioxidants CCND1 Protein Smoking OSCC Biology (General) QH301-705.5 P.S. Sushma Kaiser Jamil P. Udaykumar Fahad M. Aldakheel Shatha A. Alduraywish Bader Homoud Alali Ayesha Mateen Rabbani Syed Analysis of CCND1 protein and circulatory antioxidant enzyme activity association in oral squamous cell carcinoma
description	Antioxidants are involved in the process of cellular damage prevention, which is considered as an avenue for cancer development. Free radicals are produced in the body upon exposure to stress, cigarette smoke, alcohol, toxins found in personal care products, pesticides in foods, radiation from the sun, viruses, germs or fungi etc. CCND1/CyclinD1 protein was found to be overexpressed in Oral squamous cell carcinoma. One hundred patients with oral squamous cell carcinoma were recruited along with hundred controls for this study from MNJ institute of Oncology with the approval of Ethics Committee, 5 ml blood samples were collected from each patient and centrifuged to collect serum for various assays. The antioxidant enzymes like catalase, SOD, GPX and GST were estimated using enzymatic assays. Results were expressed as unit of activity for mg of protein. Insilco analysis is performed using STRING v 11 Protein interaction tool. The patients with oral cancer had significantly reduced activities of SOD, GST and GPX (1.49 ± 0.49, 3.97 ± 0.86 and 10.7 ± 0.73 respectively) compared to healthy controls (4.37 ± 1.43, 6.10 ± 1.12 and 13.8 ± 1.25 respectively) (p < 0.005). However no significant difference was observed with regard to catalase activity (2.71 ± 6.51 and 4.03 ± 1.48) (p = 0.28). The proteins interaction PPI enrichment p-value was found to be 3.22e-10 predicted significantly more interactions. Our research findings shown that there was a decline in activity of superoxide dismutase, glutathione peroxidase and glutathione s transferase in addition, personal habits like smoking play a major role in the development and progression of oral carcinogenesis and based on Insilco analysis results CCND1/Cyclin D1 could be the potential therapeutic target in oral squamous cell carcinoma.
format	article
author	P.S. Sushma Kaiser Jamil P. Udaykumar Fahad M. Aldakheel Shatha A. Alduraywish Bader Homoud Alali Ayesha Mateen Rabbani Syed
author_facet	P.S. Sushma Kaiser Jamil P. Udaykumar Fahad M. Aldakheel Shatha A. Alduraywish Bader Homoud Alali Ayesha Mateen Rabbani Syed
author_sort	P.S. Sushma
title	Analysis of CCND1 protein and circulatory antioxidant enzyme activity association in oral squamous cell carcinoma
title_short	Analysis of CCND1 protein and circulatory antioxidant enzyme activity association in oral squamous cell carcinoma
title_full	Analysis of CCND1 protein and circulatory antioxidant enzyme activity association in oral squamous cell carcinoma
title_fullStr	Analysis of CCND1 protein and circulatory antioxidant enzyme activity association in oral squamous cell carcinoma
title_full_unstemmed	Analysis of CCND1 protein and circulatory antioxidant enzyme activity association in oral squamous cell carcinoma
title_sort	analysis of ccnd1 protein and circulatory antioxidant enzyme activity association in oral squamous cell carcinoma
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/d59b641c41054c15bb9043933d332d67
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Analysis of CCND1 protein and circulatory antioxidant enzyme activity association in oral squamous cell carcinoma

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