Plasmid Curing and Exchange Using a Novel Counter-Selectable Marker Based on Unnatural Amino Acid Incorporation at a Sense Codon
A protocol was designed for plasmid curing using a novel counter-selectable marker, named <i>pylS<sup>ZK</sup>-pylT</i>, in <i>Escherichia coli</i>. The <i>pylS<sup>ZK</sup>-pylT</i> marker consists of the archaeal pyrrolysyl-tRNA synthetas...
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| Format: | article |
| Language: | EN |
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MDPI AG
2021
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| Online Access: | https://doaj.org/article/d59f0af6f8df45939509f832370e82ea |
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