VCAM1-α4β1 integrin interaction mediates interstitial tissue reconstruction in 3-D re-aggregate culture of dissociated prepubertal mouse testicular cells

Abstract Roles of interstitial tissue in morphogenesis of testicular structures remain less well understood. To analyze the roles of CD34+ cells in the reconstruction of interstitial tissue containing Leydig cells (LCs), and testicular structures, we used 3D-reaggregate culture of dissociated testic...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kazuko Abe, Shigeyuki Kon, Hiroki Kameyama, JiDong Zhang, Ken-ichirou Morohashi, Kenji Shimamura, Shin-ichi Abe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d5a46c7bd1d24540b2a18e3bac109b32
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d5a46c7bd1d24540b2a18e3bac109b32
record_format	dspace
spelling	oai:doaj.org-article:d5a46c7bd1d24540b2a18e3bac109b322021-12-02T15:31:27ZVCAM1-α4β1 integrin interaction mediates interstitial tissue reconstruction in 3-D re-aggregate culture of dissociated prepubertal mouse testicular cells10.1038/s41598-021-97729-y2045-2322https://doaj.org/article/d5a46c7bd1d24540b2a18e3bac109b322021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97729-yhttps://doaj.org/toc/2045-2322Abstract Roles of interstitial tissue in morphogenesis of testicular structures remain less well understood. To analyze the roles of CD34+ cells in the reconstruction of interstitial tissue containing Leydig cells (LCs), and testicular structures, we used 3D-reaggregate culture of dissociated testicular cells from prepubertal mouse. After a week of culture, adult Leydig cells (ALCs) were preferentially incorporated within CD34+ cell-aggregates, but fetal LCs (FLCs) were not. Immunofluorescence studies showed that integrins α4, α9 and β1, and VCAM1, one of the ligands for integrins α4β1 and α9β1, are expressed mainly in CD34+ cells and ALCs, but not in FLCs. Addition of function-blocking antibodies against each integrin and VCAM1 to the culture disturbed the reconstruction of testicular structures. Antibodies against α4 and β1 integrins and VCAM1 robustly inhibited cell-to-cell adhesion between testicular cells and between CD34+ cells. Cell-adhesion assays indicated that CD34+ cells adhere to VCAM1 through the interaction with α4β1 integrin. Live cell imaging showed that CD34+ cells adhered around ALC-aggregates. CD34+ cells on the dish moved toward the aggregates, extending filopodia, and entered into them, which was disturbed by VCAM1 antibody. These results indicate that VCAM1-α4β1 integrin interaction plays pivotal roles in formation of testicular interstitial tissues in vitro and also in vivo.Kazuko AbeShigeyuki KonHiroki KameyamaJiDong ZhangKen-ichirou MorohashiKenji ShimamuraShin-ichi AbeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kazuko Abe Shigeyuki Kon Hiroki Kameyama JiDong Zhang Ken-ichirou Morohashi Kenji Shimamura Shin-ichi Abe VCAM1-α4β1 integrin interaction mediates interstitial tissue reconstruction in 3-D re-aggregate culture of dissociated prepubertal mouse testicular cells
description	Abstract Roles of interstitial tissue in morphogenesis of testicular structures remain less well understood. To analyze the roles of CD34+ cells in the reconstruction of interstitial tissue containing Leydig cells (LCs), and testicular structures, we used 3D-reaggregate culture of dissociated testicular cells from prepubertal mouse. After a week of culture, adult Leydig cells (ALCs) were preferentially incorporated within CD34+ cell-aggregates, but fetal LCs (FLCs) were not. Immunofluorescence studies showed that integrins α4, α9 and β1, and VCAM1, one of the ligands for integrins α4β1 and α9β1, are expressed mainly in CD34+ cells and ALCs, but not in FLCs. Addition of function-blocking antibodies against each integrin and VCAM1 to the culture disturbed the reconstruction of testicular structures. Antibodies against α4 and β1 integrins and VCAM1 robustly inhibited cell-to-cell adhesion between testicular cells and between CD34+ cells. Cell-adhesion assays indicated that CD34+ cells adhere to VCAM1 through the interaction with α4β1 integrin. Live cell imaging showed that CD34+ cells adhered around ALC-aggregates. CD34+ cells on the dish moved toward the aggregates, extending filopodia, and entered into them, which was disturbed by VCAM1 antibody. These results indicate that VCAM1-α4β1 integrin interaction plays pivotal roles in formation of testicular interstitial tissues in vitro and also in vivo.
format	article
author	Kazuko Abe Shigeyuki Kon Hiroki Kameyama JiDong Zhang Ken-ichirou Morohashi Kenji Shimamura Shin-ichi Abe
author_facet	Kazuko Abe Shigeyuki Kon Hiroki Kameyama JiDong Zhang Ken-ichirou Morohashi Kenji Shimamura Shin-ichi Abe
author_sort	Kazuko Abe
title	VCAM1-α4β1 integrin interaction mediates interstitial tissue reconstruction in 3-D re-aggregate culture of dissociated prepubertal mouse testicular cells
title_short	VCAM1-α4β1 integrin interaction mediates interstitial tissue reconstruction in 3-D re-aggregate culture of dissociated prepubertal mouse testicular cells
title_full	VCAM1-α4β1 integrin interaction mediates interstitial tissue reconstruction in 3-D re-aggregate culture of dissociated prepubertal mouse testicular cells
title_fullStr	VCAM1-α4β1 integrin interaction mediates interstitial tissue reconstruction in 3-D re-aggregate culture of dissociated prepubertal mouse testicular cells
title_full_unstemmed	VCAM1-α4β1 integrin interaction mediates interstitial tissue reconstruction in 3-D re-aggregate culture of dissociated prepubertal mouse testicular cells
title_sort	vcam1-α4β1 integrin interaction mediates interstitial tissue reconstruction in 3-d re-aggregate culture of dissociated prepubertal mouse testicular cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d5a46c7bd1d24540b2a18e3bac109b32
work_keys_str_mv	AT kazukoabe vcam1a4b1integrininteractionmediatesinterstitialtissuereconstructionin3dreaggregatecultureofdissociatedprepubertalmousetesticularcells AT shigeyukikon vcam1a4b1integrininteractionmediatesinterstitialtissuereconstructionin3dreaggregatecultureofdissociatedprepubertalmousetesticularcells AT hirokikameyama vcam1a4b1integrininteractionmediatesinterstitialtissuereconstructionin3dreaggregatecultureofdissociatedprepubertalmousetesticularcells AT jidongzhang vcam1a4b1integrininteractionmediatesinterstitialtissuereconstructionin3dreaggregatecultureofdissociatedprepubertalmousetesticularcells AT kenichiroumorohashi vcam1a4b1integrininteractionmediatesinterstitialtissuereconstructionin3dreaggregatecultureofdissociatedprepubertalmousetesticularcells AT kenjishimamura vcam1a4b1integrininteractionmediatesinterstitialtissuereconstructionin3dreaggregatecultureofdissociatedprepubertalmousetesticularcells AT shinichiabe vcam1a4b1integrininteractionmediatesinterstitialtissuereconstructionin3dreaggregatecultureofdissociatedprepubertalmousetesticularcells
_version_	1718387173204426752

VCAM1-α4β1 integrin interaction mediates interstitial tissue reconstruction in 3-D re-aggregate culture of dissociated prepubertal mouse testicular cells

Ejemplares similares