In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9

In vivo mutagenesis systems can often show restricted capabilities and deleterious off-site mutations. Here the authors fuse base deaminases to T7 RNA polymerase to mutate a target sequence and use dCas9 to define the boundaries of the diversified DNA.

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Autores principales:	Beatriz Álvarez, Mario Mencía, Víctor de Lorenzo, Luis Ángel Fernández
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/d5ba226df2154be6944359b80f7d8ffe
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spelling	oai:doaj.org-article:d5ba226df2154be6944359b80f7d8ffe2021-12-02T14:16:47ZIn vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas910.1038/s41467-020-20230-z2041-1723https://doaj.org/article/d5ba226df2154be6944359b80f7d8ffe2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-20230-zhttps://doaj.org/toc/2041-1723In vivo mutagenesis systems can often show restricted capabilities and deleterious off-site mutations. Here the authors fuse base deaminases to T7 RNA polymerase to mutate a target sequence and use dCas9 to define the boundaries of the diversified DNA.Beatriz ÁlvarezMario MencíaVíctor de LorenzoLuis Ángel FernándezNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Beatriz Álvarez Mario Mencía Víctor de Lorenzo Luis Ángel Fernández In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9
description	In vivo mutagenesis systems can often show restricted capabilities and deleterious off-site mutations. Here the authors fuse base deaminases to T7 RNA polymerase to mutate a target sequence and use dCas9 to define the boundaries of the diversified DNA.
format	article
author	Beatriz Álvarez Mario Mencía Víctor de Lorenzo Luis Ángel Fernández
author_facet	Beatriz Álvarez Mario Mencía Víctor de Lorenzo Luis Ángel Fernández
author_sort	Beatriz Álvarez
title	In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9
title_short	In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9
title_full	In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9
title_fullStr	In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9
title_full_unstemmed	In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9
title_sort	in vivo diversification of target genomic sites using processive base deaminase fusions blocked by dcas9
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/d5ba226df2154be6944359b80f7d8ffe
work_keys_str_mv	AT beatrizalvarez invivodiversificationoftargetgenomicsitesusingprocessivebasedeaminasefusionsblockedbydcas9 AT mariomencia invivodiversificationoftargetgenomicsitesusingprocessivebasedeaminasefusionsblockedbydcas9 AT victordelorenzo invivodiversificationoftargetgenomicsitesusingprocessivebasedeaminasefusionsblockedbydcas9 AT luisangelfernandez invivodiversificationoftargetgenomicsitesusingprocessivebasedeaminasefusionsblockedbydcas9
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In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9

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