Sodium coupled bicarbonate influx regulates intracellular and apical pH in cultured rat caput epididymal epithelium.

<h4>Background</h4>The epithelium lining the epididymis provides an optimal acidic fluid microenvironment in the epididymal tract that enable spermatozoa to complete the maturation process. The present study aims to investigate the functional role of Na(+)/HCO(3)(-) cotransporter in the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wu-Lin Zuo, Sheng Li, Jie-Hong Huang, Deng-Liang Yang, Geng Zhang, Si-Liang Chen, Ye-Chun Ruan, Ke-Nan Ye, Christopher H K Cheng, Wen-Liang Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d5bb4f38b258486d80eb5208ec81c727
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d5bb4f38b258486d80eb5208ec81c727
record_format	dspace
spelling	oai:doaj.org-article:d5bb4f38b258486d80eb5208ec81c7272021-11-18T06:47:34ZSodium coupled bicarbonate influx regulates intracellular and apical pH in cultured rat caput epididymal epithelium.1932-620310.1371/journal.pone.0022283https://doaj.org/article/d5bb4f38b258486d80eb5208ec81c7272011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21887217/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The epithelium lining the epididymis provides an optimal acidic fluid microenvironment in the epididymal tract that enable spermatozoa to complete the maturation process. The present study aims to investigate the functional role of Na(+)/HCO(3)(-) cotransporter in the pH regulation in rat epididymis.<h4>Method/principal findings</h4>Immunofluorescence staining of pan cytokeratin in the primary culture of rat caput epididymal epithelium showed that the system was a suitable model for investigating the function of epididymal epithelium. Intracellular and apical pH were measured using the fluorescent pH sensitive probe carboxy-seminaphthorhodafluor-4F acetoxymethyl ester (SNARF-4F) and sparklet pH electrode respectively to explore the functional role of rat epididymal epithelium. In the HEPES buffered Krebs-Henseleit (KH) solution, the intracellular pH (pHi) recovery from NH(4)Cl induced acidification in the cultured caput epididymal epithelium was completely inhibited by amiloride, the inhibitor of Na(+)/H(+) exchanger (NHE). Immediately changing of the KH solution from HEPES buffered to HCO(3)(-) buffered would cause another pHi recovery. The pHi recovery in HCO(3)(-) buffered KH solution was inhibited by 4, 4diisothiocyanatostilbene-2,2-disulfonic acid (DIDS), the inhibitor of HCO(3)(-) transporter or by removal of extracellular Na(+). The extracellular pH measurement showed that the apical pH would increase when adding DIDS to the apical side of epididymal epithelial monolayer, however adding DIDS to the basolateral side had no effect on apical pH.<h4>Conclusions</h4>The present study shows that sodium coupled bicarbonate influx regulates intracellular and apical pH in cultured caput epididymal epithelium.Wu-Lin ZuoSheng LiJie-Hong HuangDeng-Liang YangGeng ZhangSi-Liang ChenYe-Chun RuanKe-Nan YeChristopher H K ChengWen-Liang ZhouPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e22283 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wu-Lin Zuo Sheng Li Jie-Hong Huang Deng-Liang Yang Geng Zhang Si-Liang Chen Ye-Chun Ruan Ke-Nan Ye Christopher H K Cheng Wen-Liang Zhou Sodium coupled bicarbonate influx regulates intracellular and apical pH in cultured rat caput epididymal epithelium.
description	<h4>Background</h4>The epithelium lining the epididymis provides an optimal acidic fluid microenvironment in the epididymal tract that enable spermatozoa to complete the maturation process. The present study aims to investigate the functional role of Na(+)/HCO(3)(-) cotransporter in the pH regulation in rat epididymis.<h4>Method/principal findings</h4>Immunofluorescence staining of pan cytokeratin in the primary culture of rat caput epididymal epithelium showed that the system was a suitable model for investigating the function of epididymal epithelium. Intracellular and apical pH were measured using the fluorescent pH sensitive probe carboxy-seminaphthorhodafluor-4F acetoxymethyl ester (SNARF-4F) and sparklet pH electrode respectively to explore the functional role of rat epididymal epithelium. In the HEPES buffered Krebs-Henseleit (KH) solution, the intracellular pH (pHi) recovery from NH(4)Cl induced acidification in the cultured caput epididymal epithelium was completely inhibited by amiloride, the inhibitor of Na(+)/H(+) exchanger (NHE). Immediately changing of the KH solution from HEPES buffered to HCO(3)(-) buffered would cause another pHi recovery. The pHi recovery in HCO(3)(-) buffered KH solution was inhibited by 4, 4diisothiocyanatostilbene-2,2-disulfonic acid (DIDS), the inhibitor of HCO(3)(-) transporter or by removal of extracellular Na(+). The extracellular pH measurement showed that the apical pH would increase when adding DIDS to the apical side of epididymal epithelial monolayer, however adding DIDS to the basolateral side had no effect on apical pH.<h4>Conclusions</h4>The present study shows that sodium coupled bicarbonate influx regulates intracellular and apical pH in cultured caput epididymal epithelium.
format	article
author	Wu-Lin Zuo Sheng Li Jie-Hong Huang Deng-Liang Yang Geng Zhang Si-Liang Chen Ye-Chun Ruan Ke-Nan Ye Christopher H K Cheng Wen-Liang Zhou
author_facet	Wu-Lin Zuo Sheng Li Jie-Hong Huang Deng-Liang Yang Geng Zhang Si-Liang Chen Ye-Chun Ruan Ke-Nan Ye Christopher H K Cheng Wen-Liang Zhou
author_sort	Wu-Lin Zuo
title	Sodium coupled bicarbonate influx regulates intracellular and apical pH in cultured rat caput epididymal epithelium.
title_short	Sodium coupled bicarbonate influx regulates intracellular and apical pH in cultured rat caput epididymal epithelium.
title_full	Sodium coupled bicarbonate influx regulates intracellular and apical pH in cultured rat caput epididymal epithelium.
title_fullStr	Sodium coupled bicarbonate influx regulates intracellular and apical pH in cultured rat caput epididymal epithelium.
title_full_unstemmed	Sodium coupled bicarbonate influx regulates intracellular and apical pH in cultured rat caput epididymal epithelium.
title_sort	sodium coupled bicarbonate influx regulates intracellular and apical ph in cultured rat caput epididymal epithelium.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/d5bb4f38b258486d80eb5208ec81c727
work_keys_str_mv	AT wulinzuo sodiumcoupledbicarbonateinfluxregulatesintracellularandapicalphinculturedratcaputepididymalepithelium AT shengli sodiumcoupledbicarbonateinfluxregulatesintracellularandapicalphinculturedratcaputepididymalepithelium AT jiehonghuang sodiumcoupledbicarbonateinfluxregulatesintracellularandapicalphinculturedratcaputepididymalepithelium AT dengliangyang sodiumcoupledbicarbonateinfluxregulatesintracellularandapicalphinculturedratcaputepididymalepithelium AT gengzhang sodiumcoupledbicarbonateinfluxregulatesintracellularandapicalphinculturedratcaputepididymalepithelium AT siliangchen sodiumcoupledbicarbonateinfluxregulatesintracellularandapicalphinculturedratcaputepididymalepithelium AT yechunruan sodiumcoupledbicarbonateinfluxregulatesintracellularandapicalphinculturedratcaputepididymalepithelium AT kenanye sodiumcoupledbicarbonateinfluxregulatesintracellularandapicalphinculturedratcaputepididymalepithelium AT christopherhkcheng sodiumcoupledbicarbonateinfluxregulatesintracellularandapicalphinculturedratcaputepididymalepithelium AT wenliangzhou sodiumcoupledbicarbonateinfluxregulatesintracellularandapicalphinculturedratcaputepididymalepithelium
_version_	1718424382300225536

Sodium coupled bicarbonate influx regulates intracellular and apical pH in cultured rat caput epididymal epithelium.

Ejemplares similares