Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction

The detection of pathogens in food and clinical samples remains a challenge. Here, Shen et al. present a detection system, involving a combination of nucleic acid-based allosteric probes and CRISPR-Cas13a components, that can detect very low numbers of a bacterial pathogen in milk and serum samples...

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Autores principales:	Jinjin Shen, Xiaoming Zhou, Yuanyue Shan, Huahua Yue, Ru Huang, Jiaming Hu, Da Xing
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/d5be5932d63d44d082e55efb605ab928
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id	oai:doaj.org-article:d5be5932d63d44d082e55efb605ab928
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spelling	oai:doaj.org-article:d5be5932d63d44d082e55efb605ab9282021-12-02T16:50:04ZSensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction10.1038/s41467-019-14135-92041-1723https://doaj.org/article/d5be5932d63d44d082e55efb605ab9282020-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-14135-9https://doaj.org/toc/2041-1723The detection of pathogens in food and clinical samples remains a challenge. Here, Shen et al. present a detection system, involving a combination of nucleic acid-based allosteric probes and CRISPR-Cas13a components, that can detect very low numbers of a bacterial pathogen in milk and serum samples without isolation.Jinjin ShenXiaoming ZhouYuanyue ShanHuahua YueRu HuangJiaming HuDa XingNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jinjin Shen Xiaoming Zhou Yuanyue Shan Huahua Yue Ru Huang Jiaming Hu Da Xing Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction
description	The detection of pathogens in food and clinical samples remains a challenge. Here, Shen et al. present a detection system, involving a combination of nucleic acid-based allosteric probes and CRISPR-Cas13a components, that can detect very low numbers of a bacterial pathogen in milk and serum samples without isolation.
format	article
author	Jinjin Shen Xiaoming Zhou Yuanyue Shan Huahua Yue Ru Huang Jiaming Hu Da Xing
author_facet	Jinjin Shen Xiaoming Zhou Yuanyue Shan Huahua Yue Ru Huang Jiaming Hu Da Xing
author_sort	Jinjin Shen
title	Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction
title_short	Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction
title_full	Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction
title_fullStr	Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction
title_full_unstemmed	Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction
title_sort	sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and crispr-cas13a amplification reaction
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/d5be5932d63d44d082e55efb605ab928
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Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction

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