Green Pea (<i>Pisum sativum</i> L.) Hull Polyphenol Extracts Ameliorate DSS-Induced Colitis through Keap1/Nrf2 Pathway and Gut Microbiota Modulation

As a processing by-product, green pea hull (GPH) was found to be rich in phenolic components in our previous studies. In this study, UHPLC-LTQ-OrbiTrap-MS (Ultra performance liquid chromatography-linear ion trap orbitrap tandem mass spectrometry) technique was used to quantify polyphenols, and DSS (...

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Autores principales:	Fanghua Guo, Rong Tsao, Chuyao Li, Xiaoya Wang, Hua Zhang, Li Jiang, Yong Sun, Hua Xiong
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	polyphenols colitis UHPLC-LTQ-OrbiTrap-MS GPH extracts Keap1-Nrf2 gut microbiota Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/d5c1ac1b9cfe465baff72e82af8039d2
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spelling	oai:doaj.org-article:d5c1ac1b9cfe465baff72e82af8039d22021-11-25T17:35:23ZGreen Pea (<i>Pisum sativum</i> L.) Hull Polyphenol Extracts Ameliorate DSS-Induced Colitis through Keap1/Nrf2 Pathway and Gut Microbiota Modulation10.3390/foods101127652304-8158https://doaj.org/article/d5c1ac1b9cfe465baff72e82af8039d22021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2765https://doaj.org/toc/2304-8158As a processing by-product, green pea hull (GPH) was found to be rich in phenolic components in our previous studies. In this study, UHPLC-LTQ-OrbiTrap-MS (Ultra performance liquid chromatography-linear ion trap orbitrap tandem mass spectrometry) technique was used to quantify polyphenols, and DSS (sodium dextran sulfate)-induced colitis mouse model was established to explore the effect of GPH extracts on colitis. The results showed that quercetin and its derivatives, kaempferol trihexanside and catechin and its derivatives were the main phenolic substances in the extract, reaching 2836.57, 1482.00 and 1339.91 µg quercetin/g GPH extract, respectively; GPH extracts can improved inflammatory status, repaired colonic function, regulated inflammatory factors, and restored oxidative balance in mice. Further, GPH extracts can activate Keap1-Nrf2-ARE signaling pathway, regulate downstream antioxidant protease and gut microbiota by increasing F/B value and promoting the growth of <i>Lactobacillaceae</i> and <i>Lachnospiraceae</i>, and improve the level of SCFAs (short-chain fatty acids) to relieve DSS-induced colitis in mice. Therefore, GPH may be a promising dietary resource for the treatment of ulcerative colitis.Fanghua GuoRong TsaoChuyao LiXiaoya WangHua ZhangLi JiangYong SunHua XiongMDPI AGarticlepolyphenolscolitisUHPLC-LTQ-OrbiTrap-MSGPH extractsKeap1-Nrf2gut microbiotaChemical technologyTP1-1185ENFoods, Vol 10, Iss 2765, p 2765 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	polyphenols colitis UHPLC-LTQ-OrbiTrap-MS GPH extracts Keap1-Nrf2 gut microbiota Chemical technology TP1-1185
spellingShingle	polyphenols colitis UHPLC-LTQ-OrbiTrap-MS GPH extracts Keap1-Nrf2 gut microbiota Chemical technology TP1-1185 Fanghua Guo Rong Tsao Chuyao Li Xiaoya Wang Hua Zhang Li Jiang Yong Sun Hua Xiong Green Pea (<i>Pisum sativum</i> L.) Hull Polyphenol Extracts Ameliorate DSS-Induced Colitis through Keap1/Nrf2 Pathway and Gut Microbiota Modulation
description	As a processing by-product, green pea hull (GPH) was found to be rich in phenolic components in our previous studies. In this study, UHPLC-LTQ-OrbiTrap-MS (Ultra performance liquid chromatography-linear ion trap orbitrap tandem mass spectrometry) technique was used to quantify polyphenols, and DSS (sodium dextran sulfate)-induced colitis mouse model was established to explore the effect of GPH extracts on colitis. The results showed that quercetin and its derivatives, kaempferol trihexanside and catechin and its derivatives were the main phenolic substances in the extract, reaching 2836.57, 1482.00 and 1339.91 µg quercetin/g GPH extract, respectively; GPH extracts can improved inflammatory status, repaired colonic function, regulated inflammatory factors, and restored oxidative balance in mice. Further, GPH extracts can activate Keap1-Nrf2-ARE signaling pathway, regulate downstream antioxidant protease and gut microbiota by increasing F/B value and promoting the growth of <i>Lactobacillaceae</i> and <i>Lachnospiraceae</i>, and improve the level of SCFAs (short-chain fatty acids) to relieve DSS-induced colitis in mice. Therefore, GPH may be a promising dietary resource for the treatment of ulcerative colitis.
format	article
author	Fanghua Guo Rong Tsao Chuyao Li Xiaoya Wang Hua Zhang Li Jiang Yong Sun Hua Xiong
author_facet	Fanghua Guo Rong Tsao Chuyao Li Xiaoya Wang Hua Zhang Li Jiang Yong Sun Hua Xiong
author_sort	Fanghua Guo
title	Green Pea (<i>Pisum sativum</i> L.) Hull Polyphenol Extracts Ameliorate DSS-Induced Colitis through Keap1/Nrf2 Pathway and Gut Microbiota Modulation
title_short	Green Pea (<i>Pisum sativum</i> L.) Hull Polyphenol Extracts Ameliorate DSS-Induced Colitis through Keap1/Nrf2 Pathway and Gut Microbiota Modulation
title_full	Green Pea (<i>Pisum sativum</i> L.) Hull Polyphenol Extracts Ameliorate DSS-Induced Colitis through Keap1/Nrf2 Pathway and Gut Microbiota Modulation
title_fullStr	Green Pea (<i>Pisum sativum</i> L.) Hull Polyphenol Extracts Ameliorate DSS-Induced Colitis through Keap1/Nrf2 Pathway and Gut Microbiota Modulation
title_full_unstemmed	Green Pea (<i>Pisum sativum</i> L.) Hull Polyphenol Extracts Ameliorate DSS-Induced Colitis through Keap1/Nrf2 Pathway and Gut Microbiota Modulation
title_sort	green pea (<i>pisum sativum</i> l.) hull polyphenol extracts ameliorate dss-induced colitis through keap1/nrf2 pathway and gut microbiota modulation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d5c1ac1b9cfe465baff72e82af8039d2
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Green Pea (<i>Pisum sativum</i> L.) Hull Polyphenol Extracts Ameliorate DSS-Induced Colitis through Keap1/Nrf2 Pathway and Gut Microbiota Modulation

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