Effect of GOLPH3 on cumulus granulosa cell apoptosis and ICSI pregnancy outcomes

Abstract Previous studies have shown that GOLPH3 mediates cell growth, proliferation and differentiation and inhibits cell apoptosis; however, the role of GOLPH3 in cumulus granulosa cells and the value of GOLPH3 in predicting ICSI pregnancy outcomes remain unknown until now. Our findings showed hig...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dianliang Lin, Jing Ran, Suqin Zhu, Song Quan, Baofeng Ye, Aili Yu, Yuefan Kang, Yuan Lin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d5c5f6707dd149fca68338ff5896f64c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d5c5f6707dd149fca68338ff5896f64c
record_format	dspace
spelling	oai:doaj.org-article:d5c5f6707dd149fca68338ff5896f64c2021-12-02T12:32:26ZEffect of GOLPH3 on cumulus granulosa cell apoptosis and ICSI pregnancy outcomes10.1038/s41598-017-08343-w2045-2322https://doaj.org/article/d5c5f6707dd149fca68338ff5896f64c2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08343-whttps://doaj.org/toc/2045-2322Abstract Previous studies have shown that GOLPH3 mediates cell growth, proliferation and differentiation and inhibits cell apoptosis; however, the role of GOLPH3 in cumulus granulosa cells and the value of GOLPH3 in predicting ICSI pregnancy outcomes remain unknown until now. Our findings showed higher positive expression rate, score of staining intensity, and immunohistochemical score of GOLPH3 in the cumulus granulosa cells of the pregnant women relative to non-pregnant women, and a higher apoptotic rate of cumulus granulosa cells was detected in non-pregnant women than in pregnant women. Pearson correlation analyses revealed that pregnancy correlated negatively with GOLPH3 expression and apoptosis of cumulus granulosa cells, and positively with the number of follicles punctured, number of grade III oocytes, number of eggs retrieved for ICSI, number of zygotes, number of cleavage-stage embryos, number of top-quality embryos, number of blastocysts, number of top-quality blastocysts, and number of frozen embryos. GOLPH3 may be involved in the apoptosis of cumulus granulosa cells, which may correlate with oocyte maturation and egg development. GOLPH3 expression in cumulus granulosa cells may facilitate the selection of top-quality eggs and embryos, the prediction of the clinical pregnancy outcomes of ICSI, and the increase of the pregnancy rate.Dianliang LinJing RanSuqin ZhuSong QuanBaofeng YeAili YuYuefan KangYuan LinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dianliang Lin Jing Ran Suqin Zhu Song Quan Baofeng Ye Aili Yu Yuefan Kang Yuan Lin Effect of GOLPH3 on cumulus granulosa cell apoptosis and ICSI pregnancy outcomes
description	Abstract Previous studies have shown that GOLPH3 mediates cell growth, proliferation and differentiation and inhibits cell apoptosis; however, the role of GOLPH3 in cumulus granulosa cells and the value of GOLPH3 in predicting ICSI pregnancy outcomes remain unknown until now. Our findings showed higher positive expression rate, score of staining intensity, and immunohistochemical score of GOLPH3 in the cumulus granulosa cells of the pregnant women relative to non-pregnant women, and a higher apoptotic rate of cumulus granulosa cells was detected in non-pregnant women than in pregnant women. Pearson correlation analyses revealed that pregnancy correlated negatively with GOLPH3 expression and apoptosis of cumulus granulosa cells, and positively with the number of follicles punctured, number of grade III oocytes, number of eggs retrieved for ICSI, number of zygotes, number of cleavage-stage embryos, number of top-quality embryos, number of blastocysts, number of top-quality blastocysts, and number of frozen embryos. GOLPH3 may be involved in the apoptosis of cumulus granulosa cells, which may correlate with oocyte maturation and egg development. GOLPH3 expression in cumulus granulosa cells may facilitate the selection of top-quality eggs and embryos, the prediction of the clinical pregnancy outcomes of ICSI, and the increase of the pregnancy rate.
format	article
author	Dianliang Lin Jing Ran Suqin Zhu Song Quan Baofeng Ye Aili Yu Yuefan Kang Yuan Lin
author_facet	Dianliang Lin Jing Ran Suqin Zhu Song Quan Baofeng Ye Aili Yu Yuefan Kang Yuan Lin
author_sort	Dianliang Lin
title	Effect of GOLPH3 on cumulus granulosa cell apoptosis and ICSI pregnancy outcomes
title_short	Effect of GOLPH3 on cumulus granulosa cell apoptosis and ICSI pregnancy outcomes
title_full	Effect of GOLPH3 on cumulus granulosa cell apoptosis and ICSI pregnancy outcomes
title_fullStr	Effect of GOLPH3 on cumulus granulosa cell apoptosis and ICSI pregnancy outcomes
title_full_unstemmed	Effect of GOLPH3 on cumulus granulosa cell apoptosis and ICSI pregnancy outcomes
title_sort	effect of golph3 on cumulus granulosa cell apoptosis and icsi pregnancy outcomes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d5c5f6707dd149fca68338ff5896f64c
work_keys_str_mv	AT dianlianglin effectofgolph3oncumulusgranulosacellapoptosisandicsipregnancyoutcomes AT jingran effectofgolph3oncumulusgranulosacellapoptosisandicsipregnancyoutcomes AT suqinzhu effectofgolph3oncumulusgranulosacellapoptosisandicsipregnancyoutcomes AT songquan effectofgolph3oncumulusgranulosacellapoptosisandicsipregnancyoutcomes AT baofengye effectofgolph3oncumulusgranulosacellapoptosisandicsipregnancyoutcomes AT ailiyu effectofgolph3oncumulusgranulosacellapoptosisandicsipregnancyoutcomes AT yuefankang effectofgolph3oncumulusgranulosacellapoptosisandicsipregnancyoutcomes AT yuanlin effectofgolph3oncumulusgranulosacellapoptosisandicsipregnancyoutcomes
_version_	1718394065616109568

Effect of GOLPH3 on cumulus granulosa cell apoptosis and ICSI pregnancy outcomes

Ejemplares similares