Nanostructured Plasma Polymerized Fluorocarbon Films for Drop Coating Deposition Raman Spectroscopy (DCDRS) of Liposomes

Raman spectroscopy is one of the most used biodetection techniques. However, its usability is hampered in the case of low concentrated substances because of the weak intensity of the Raman signal. To overcome this limitation, the use of drop coating deposition Raman spectroscopy (DCDRS), in which th...

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Autores principales:	Alžbeta Kuižová, Anna Kuzminova, Ondřej Kylián, Eva Kočišová
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	drop coating deposition Raman spectroscopy DCDRS plasma polymers liposomes nanostructured surfaces wettability Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/d5c740977aaa43d1aa8741fef615bb35
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id	oai:doaj.org-article:d5c740977aaa43d1aa8741fef615bb35
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spelling	oai:doaj.org-article:d5c740977aaa43d1aa8741fef615bb352021-11-25T18:49:28ZNanostructured Plasma Polymerized Fluorocarbon Films for Drop Coating Deposition Raman Spectroscopy (DCDRS) of Liposomes10.3390/polym132240232073-4360https://doaj.org/article/d5c740977aaa43d1aa8741fef615bb352021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/22/4023https://doaj.org/toc/2073-4360Raman spectroscopy is one of the most used biodetection techniques. However, its usability is hampered in the case of low concentrated substances because of the weak intensity of the Raman signal. To overcome this limitation, the use of drop coating deposition Raman spectroscopy (DCDRS), in which the liquid samples are allowed to dry into well-defined patterns where the non-volatile solutes are highly concentrated, is appropriate. This significantly improves the Raman sensitivity when compared to the conventional Raman signal from solution/suspension. As DCDRS performance strongly depends on the wetting properties of substrates, we demonstrate here that the smooth hydrophobic plasma polymerized fluorocarbon films prepared by magnetron sputtering (contact angle 108°) are well-suited for the DCDRS detection of liposomes. Furthermore, it was proved that even better improvement of the Raman signal might be achieved if the plasma polymer surfaces are roughened. In this case, 100% higher intensities of Raman signal are observed in comparison with smooth fluorocarbon films. As it is shown, this effect, which has no influence on the profile of Raman spectra, is connected with the increased hydrophobicity of nanostructured fluorocarbon films. This results in the formation of dried liposomal deposits with smaller diameters and higher preconcentration of liposomes.Alžbeta KuižováAnna KuzminovaOndřej KyliánEva KočišováMDPI AGarticledrop coating deposition Raman spectroscopyDCDRSplasma polymersliposomesnanostructured surfaceswettabilityOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 4023, p 4023 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	drop coating deposition Raman spectroscopy DCDRS plasma polymers liposomes nanostructured surfaces wettability Organic chemistry QD241-441
spellingShingle	drop coating deposition Raman spectroscopy DCDRS plasma polymers liposomes nanostructured surfaces wettability Organic chemistry QD241-441 Alžbeta Kuižová Anna Kuzminova Ondřej Kylián Eva Kočišová Nanostructured Plasma Polymerized Fluorocarbon Films for Drop Coating Deposition Raman Spectroscopy (DCDRS) of Liposomes
description	Raman spectroscopy is one of the most used biodetection techniques. However, its usability is hampered in the case of low concentrated substances because of the weak intensity of the Raman signal. To overcome this limitation, the use of drop coating deposition Raman spectroscopy (DCDRS), in which the liquid samples are allowed to dry into well-defined patterns where the non-volatile solutes are highly concentrated, is appropriate. This significantly improves the Raman sensitivity when compared to the conventional Raman signal from solution/suspension. As DCDRS performance strongly depends on the wetting properties of substrates, we demonstrate here that the smooth hydrophobic plasma polymerized fluorocarbon films prepared by magnetron sputtering (contact angle 108°) are well-suited for the DCDRS detection of liposomes. Furthermore, it was proved that even better improvement of the Raman signal might be achieved if the plasma polymer surfaces are roughened. In this case, 100% higher intensities of Raman signal are observed in comparison with smooth fluorocarbon films. As it is shown, this effect, which has no influence on the profile of Raman spectra, is connected with the increased hydrophobicity of nanostructured fluorocarbon films. This results in the formation of dried liposomal deposits with smaller diameters and higher preconcentration of liposomes.
format	article
author	Alžbeta Kuižová Anna Kuzminova Ondřej Kylián Eva Kočišová
author_facet	Alžbeta Kuižová Anna Kuzminova Ondřej Kylián Eva Kočišová
author_sort	Alžbeta Kuižová
title	Nanostructured Plasma Polymerized Fluorocarbon Films for Drop Coating Deposition Raman Spectroscopy (DCDRS) of Liposomes
title_short	Nanostructured Plasma Polymerized Fluorocarbon Films for Drop Coating Deposition Raman Spectroscopy (DCDRS) of Liposomes
title_full	Nanostructured Plasma Polymerized Fluorocarbon Films for Drop Coating Deposition Raman Spectroscopy (DCDRS) of Liposomes
title_fullStr	Nanostructured Plasma Polymerized Fluorocarbon Films for Drop Coating Deposition Raman Spectroscopy (DCDRS) of Liposomes
title_full_unstemmed	Nanostructured Plasma Polymerized Fluorocarbon Films for Drop Coating Deposition Raman Spectroscopy (DCDRS) of Liposomes
title_sort	nanostructured plasma polymerized fluorocarbon films for drop coating deposition raman spectroscopy (dcdrs) of liposomes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d5c740977aaa43d1aa8741fef615bb35
work_keys_str_mv	AT alzbetakuizova nanostructuredplasmapolymerizedfluorocarbonfilmsfordropcoatingdepositionramanspectroscopydcdrsofliposomes AT annakuzminova nanostructuredplasmapolymerizedfluorocarbonfilmsfordropcoatingdepositionramanspectroscopydcdrsofliposomes AT ondrejkylian nanostructuredplasmapolymerizedfluorocarbonfilmsfordropcoatingdepositionramanspectroscopydcdrsofliposomes AT evakocisova nanostructuredplasmapolymerizedfluorocarbonfilmsfordropcoatingdepositionramanspectroscopydcdrsofliposomes
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Nanostructured Plasma Polymerized Fluorocarbon Films for Drop Coating Deposition Raman Spectroscopy (DCDRS) of Liposomes

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