An In-Vitro Study of Molecular Effects of a Combination Treat-ment with Antibiotics and Nanofluid Containing Carbon Nano-tubes on Klebsiella pneumoniae

Background: We aimed to prepare a nanofluid, containing f-MWCNTs, and investigate the antibacterial efficacy of f-MWCNTs+ ciprofloxacin (cip) on Klebsiella pneumoniae by evaluating the virulence gene expression. Methods: This study was carried out from 2019 to 2020, in the Department of Mycobact...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Maryam Mehdizadeh, Mojgan Sheikhpour, Iman Salahshourifar, Seyed Davar Siadat, Parvaneh Saffarian
Formato:	article
Lenguaje:	EN
Publicado:	Tehran University of Medical Sciences 2021
Materias:	Carbon nanotube Functionalized MWCNT Klebsiella pneumoniae Virulence genes Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/d5cabc81af794a8887e2fa58701fc42d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d5cabc81af794a8887e2fa58701fc42d
record_format	dspace
spelling	oai:doaj.org-article:d5cabc81af794a8887e2fa58701fc42d2021-12-02T18:23:25ZAn In-Vitro Study of Molecular Effects of a Combination Treat-ment with Antibiotics and Nanofluid Containing Carbon Nano-tubes on Klebsiella pneumoniae2251-60852251-6093https://doaj.org/article/d5cabc81af794a8887e2fa58701fc42d2021-10-01T00:00:00Zhttps://ijph.tums.ac.ir/index.php/ijph/article/view/25850https://doaj.org/toc/2251-6085https://doaj.org/toc/2251-6093 Background: We aimed to prepare a nanofluid, containing f-MWCNTs, and investigate the antibacterial efficacy of f-MWCNTs+ ciprofloxacin (cip) on Klebsiella pneumoniae by evaluating the virulence gene expression. Methods: This study was carried out from 2019 to 2020, in the Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran. The nanofluid containing antibiotic and f-MWCNTs were prepared by the ultrasonic method. The minimum inhibitory concentrations (MICs) of ciprofloxacin and f-MWCNTs were determined using the broth micro dilution MIC tests. For examining the antibacterial effects, the expression level of virulence genes, under the influence of f-MWCNTs, was evaluated by a real-time PCR. Results: The effect of 8 µg/ml ciprofloxacin + 400 µg/ml f-MWCNTs, completely inhibited the growth of the resistant isolate of K. pneumoniae, while, in the ATCC 700,603 isolate, 2 µg/ml ciprofloxacin with 100 µg/ml f-MWCNT could inhibit a bacterial growth. In the resistant K. pneumoniae clinical isolate, after f-MWCNT+cip treatment, the expression of fimA, fimD, wza, and wzi genes was significantly downregulated, compared to the ciprofloxacin treatment, and upregulated, compared to the negative control. For the ATCC 700,603 isolate treated with f-MWCNT+cip, the expression of fimA, fimD and wza virulence genes showed upregulation, compared to the negative control and downregulated in comparison with the ciprofloxacin treatment. Conclusion: Simultaneous treatment of resistant isolate of K. pneumoniae with f-MWCNTs +antibiotic could improve the effectiveness of antibiotic at lower doses, due to the reduced expression of virulence genes in comparison with antibiotic treatment, besides the increased cell wall permeability to antibiotics. Maryam MehdizadehMojgan SheikhpourIman SalahshourifarSeyed Davar SiadatParvaneh SaffarianTehran University of Medical SciencesarticleCarbon nanotubeFunctionalized MWCNTKlebsiella pneumoniaeVirulence genesPublic aspects of medicineRA1-1270ENIranian Journal of Public Health, Vol 50, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Carbon nanotube Functionalized MWCNT Klebsiella pneumoniae Virulence genes Public aspects of medicine RA1-1270
spellingShingle	Carbon nanotube Functionalized MWCNT Klebsiella pneumoniae Virulence genes Public aspects of medicine RA1-1270 Maryam Mehdizadeh Mojgan Sheikhpour Iman Salahshourifar Seyed Davar Siadat Parvaneh Saffarian An In-Vitro Study of Molecular Effects of a Combination Treat-ment with Antibiotics and Nanofluid Containing Carbon Nano-tubes on Klebsiella pneumoniae
description	Background: We aimed to prepare a nanofluid, containing f-MWCNTs, and investigate the antibacterial efficacy of f-MWCNTs+ ciprofloxacin (cip) on Klebsiella pneumoniae by evaluating the virulence gene expression. Methods: This study was carried out from 2019 to 2020, in the Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran. The nanofluid containing antibiotic and f-MWCNTs were prepared by the ultrasonic method. The minimum inhibitory concentrations (MICs) of ciprofloxacin and f-MWCNTs were determined using the broth micro dilution MIC tests. For examining the antibacterial effects, the expression level of virulence genes, under the influence of f-MWCNTs, was evaluated by a real-time PCR. Results: The effect of 8 µg/ml ciprofloxacin + 400 µg/ml f-MWCNTs, completely inhibited the growth of the resistant isolate of K. pneumoniae, while, in the ATCC 700,603 isolate, 2 µg/ml ciprofloxacin with 100 µg/ml f-MWCNT could inhibit a bacterial growth. In the resistant K. pneumoniae clinical isolate, after f-MWCNT+cip treatment, the expression of fimA, fimD, wza, and wzi genes was significantly downregulated, compared to the ciprofloxacin treatment, and upregulated, compared to the negative control. For the ATCC 700,603 isolate treated with f-MWCNT+cip, the expression of fimA, fimD and wza virulence genes showed upregulation, compared to the negative control and downregulated in comparison with the ciprofloxacin treatment. Conclusion: Simultaneous treatment of resistant isolate of K. pneumoniae with f-MWCNTs +antibiotic could improve the effectiveness of antibiotic at lower doses, due to the reduced expression of virulence genes in comparison with antibiotic treatment, besides the increased cell wall permeability to antibiotics.
format	article
author	Maryam Mehdizadeh Mojgan Sheikhpour Iman Salahshourifar Seyed Davar Siadat Parvaneh Saffarian
author_facet	Maryam Mehdizadeh Mojgan Sheikhpour Iman Salahshourifar Seyed Davar Siadat Parvaneh Saffarian
author_sort	Maryam Mehdizadeh
title	An In-Vitro Study of Molecular Effects of a Combination Treat-ment with Antibiotics and Nanofluid Containing Carbon Nano-tubes on Klebsiella pneumoniae
title_short	An In-Vitro Study of Molecular Effects of a Combination Treat-ment with Antibiotics and Nanofluid Containing Carbon Nano-tubes on Klebsiella pneumoniae
title_full	An In-Vitro Study of Molecular Effects of a Combination Treat-ment with Antibiotics and Nanofluid Containing Carbon Nano-tubes on Klebsiella pneumoniae
title_fullStr	An In-Vitro Study of Molecular Effects of a Combination Treat-ment with Antibiotics and Nanofluid Containing Carbon Nano-tubes on Klebsiella pneumoniae
title_full_unstemmed	An In-Vitro Study of Molecular Effects of a Combination Treat-ment with Antibiotics and Nanofluid Containing Carbon Nano-tubes on Klebsiella pneumoniae
title_sort	in-vitro study of molecular effects of a combination treat-ment with antibiotics and nanofluid containing carbon nano-tubes on klebsiella pneumoniae
publisher	Tehran University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/d5cabc81af794a8887e2fa58701fc42d
work_keys_str_mv	AT maryammehdizadeh aninvitrostudyofmoleculareffectsofacombinationtreatmentwithantibioticsandnanofluidcontainingcarbonnanotubesonklebsiellapneumoniae AT mojgansheikhpour aninvitrostudyofmoleculareffectsofacombinationtreatmentwithantibioticsandnanofluidcontainingcarbonnanotubesonklebsiellapneumoniae AT imansalahshourifar aninvitrostudyofmoleculareffectsofacombinationtreatmentwithantibioticsandnanofluidcontainingcarbonnanotubesonklebsiellapneumoniae AT seyeddavarsiadat aninvitrostudyofmoleculareffectsofacombinationtreatmentwithantibioticsandnanofluidcontainingcarbonnanotubesonklebsiellapneumoniae AT parvanehsaffarian aninvitrostudyofmoleculareffectsofacombinationtreatmentwithantibioticsandnanofluidcontainingcarbonnanotubesonklebsiellapneumoniae AT maryammehdizadeh invitrostudyofmoleculareffectsofacombinationtreatmentwithantibioticsandnanofluidcontainingcarbonnanotubesonklebsiellapneumoniae AT mojgansheikhpour invitrostudyofmoleculareffectsofacombinationtreatmentwithantibioticsandnanofluidcontainingcarbonnanotubesonklebsiellapneumoniae AT imansalahshourifar invitrostudyofmoleculareffectsofacombinationtreatmentwithantibioticsandnanofluidcontainingcarbonnanotubesonklebsiellapneumoniae AT seyeddavarsiadat invitrostudyofmoleculareffectsofacombinationtreatmentwithantibioticsandnanofluidcontainingcarbonnanotubesonklebsiellapneumoniae AT parvanehsaffarian invitrostudyofmoleculareffectsofacombinationtreatmentwithantibioticsandnanofluidcontainingcarbonnanotubesonklebsiellapneumoniae
_version_	1718378127160246272

An In-Vitro Study of Molecular Effects of a Combination Treat-ment with Antibiotics and Nanofluid Containing Carbon Nano-tubes on Klebsiella pneumoniae

Ejemplares similares