Effect of CXCL12/CXCR4 signaling on neuropathic pain after chronic compression of dorsal root ganglion

Abstract Neuropathic pain is a complex, chronic pain state that often accompanies tissue damage, inflammation or injury of the nervous system. However the underlying molecular mechanisms still remain unclear. Here, we showed that CXCL12 and CXCR4 were upregulated in the dorsal root ganglion (DRG) af...

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Autores principales:	Yang Yu, Xini Huang, Yuwei Di, Lintao Qu, Ni Fan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d5cae1f35fa140c49870ff674157cdc2
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spelling	oai:doaj.org-article:d5cae1f35fa140c49870ff674157cdc22021-12-02T12:32:14ZEffect of CXCL12/CXCR4 signaling on neuropathic pain after chronic compression of dorsal root ganglion10.1038/s41598-017-05954-12045-2322https://doaj.org/article/d5cae1f35fa140c49870ff674157cdc22017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05954-1https://doaj.org/toc/2045-2322Abstract Neuropathic pain is a complex, chronic pain state that often accompanies tissue damage, inflammation or injury of the nervous system. However the underlying molecular mechanisms still remain unclear. Here, we showed that CXCL12 and CXCR4 were upregulated in the dorsal root ganglion (DRG) after chronic compression of DRG (CCD), and some CXCR4 immunopositive neurons were also immunopositive for the nociceptive neuronal markers IB4, TRPV1, CGRP, and substance P. The incidence and amplitude of CXCL12-induced Ca2+ response in primary sensory neurons from CCD mice was significantly increased compared to those from control animals. CXCL12 depolarized the resting membrane potential, decreased the rheobase, and increased the number of action potentials evoked by a depolarizing current at 2X rheobase in neurons from CCD mice. The mechanical and thermal hypernociception after CCD was attenuated by administration of a CXCR4 antagonist AMD3100. These findings suggest that CXCL12/CXCR4 signaling contributes to hypernociception after CCD, and targeting CXCL12/CXCR4 signaling pathway may alleviate neuropathic pain.Yang YuXini HuangYuwei DiLintao QuNi FanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yang Yu Xini Huang Yuwei Di Lintao Qu Ni Fan Effect of CXCL12/CXCR4 signaling on neuropathic pain after chronic compression of dorsal root ganglion
description	Abstract Neuropathic pain is a complex, chronic pain state that often accompanies tissue damage, inflammation or injury of the nervous system. However the underlying molecular mechanisms still remain unclear. Here, we showed that CXCL12 and CXCR4 were upregulated in the dorsal root ganglion (DRG) after chronic compression of DRG (CCD), and some CXCR4 immunopositive neurons were also immunopositive for the nociceptive neuronal markers IB4, TRPV1, CGRP, and substance P. The incidence and amplitude of CXCL12-induced Ca2+ response in primary sensory neurons from CCD mice was significantly increased compared to those from control animals. CXCL12 depolarized the resting membrane potential, decreased the rheobase, and increased the number of action potentials evoked by a depolarizing current at 2X rheobase in neurons from CCD mice. The mechanical and thermal hypernociception after CCD was attenuated by administration of a CXCR4 antagonist AMD3100. These findings suggest that CXCL12/CXCR4 signaling contributes to hypernociception after CCD, and targeting CXCL12/CXCR4 signaling pathway may alleviate neuropathic pain.
format	article
author	Yang Yu Xini Huang Yuwei Di Lintao Qu Ni Fan
author_facet	Yang Yu Xini Huang Yuwei Di Lintao Qu Ni Fan
author_sort	Yang Yu
title	Effect of CXCL12/CXCR4 signaling on neuropathic pain after chronic compression of dorsal root ganglion
title_short	Effect of CXCL12/CXCR4 signaling on neuropathic pain after chronic compression of dorsal root ganglion
title_full	Effect of CXCL12/CXCR4 signaling on neuropathic pain after chronic compression of dorsal root ganglion
title_fullStr	Effect of CXCL12/CXCR4 signaling on neuropathic pain after chronic compression of dorsal root ganglion
title_full_unstemmed	Effect of CXCL12/CXCR4 signaling on neuropathic pain after chronic compression of dorsal root ganglion
title_sort	effect of cxcl12/cxcr4 signaling on neuropathic pain after chronic compression of dorsal root ganglion
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d5cae1f35fa140c49870ff674157cdc2
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_version_	1718394184788869120

Effect of CXCL12/CXCR4 signaling on neuropathic pain after chronic compression of dorsal root ganglion

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