Role of FoxO1 and apoptosis in pulmonary vascular remolding in a rat model of chronic thromboembolic pulmonary hypertension

Abstract To explore the role of FoxO1 and apoptosis in a rat model of chronic thromboembolic pulmonary hypertension (CTEPH). Rats were randomly divided into a sham group (n = 45) and an experimental group (n = 45). Autologous blood clots were injected into rats three times to induce CTEPH. Rats were...

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Autores principales:	Chaosheng Deng, Zhanghua Zhong, Dawen Wu, Yunfei Chen, Ningfang Lian, Haibo Ding, Qiaoxian Zhang, Qichang Lin, Shuang Wu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d5d19a3198554d49b1614cbb868a2ac9
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spelling	oai:doaj.org-article:d5d19a3198554d49b1614cbb868a2ac92021-12-02T15:18:52ZRole of FoxO1 and apoptosis in pulmonary vascular remolding in a rat model of chronic thromboembolic pulmonary hypertension10.1038/s41598-017-02007-52045-2322https://doaj.org/article/d5d19a3198554d49b1614cbb868a2ac92017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02007-5https://doaj.org/toc/2045-2322Abstract To explore the role of FoxO1 and apoptosis in a rat model of chronic thromboembolic pulmonary hypertension (CTEPH). Rats were randomly divided into a sham group (n = 45) and an experimental group (n = 45). Autologous blood clots were injected into rats three times to induce CTEPH. Rats were further divided into three subgroups: a 1-week subgroup (n = 15), a 2-week subgroup (n = 15), and a 4-week subgroup (n = 15). Mean pulmonary arterial pressure (mPAP) and histopathology were evaluated at each time point. FoxO1, Bad, and Bcl-2 levels were examined at each time point using reverse transcription PCR and western blotting. The mPAP and vessel wall area/total area (WA/TA) ratio in the experimental group gradually increased in a time-dependent manner (P < 0.05). Both the mRNA and protein levels of FoxO1 decreased in the CTEPH rats compared to in the sham group. In addition, embolization led to the up-regulation of Bad and the down-regulation of Bcl-2 (P < 0.05). FoxO1 and apoptosis play an important role in the pathogenesis of CTEPH. Apoptosis-resistant pulmonary artery endothelial cells may play an important role in remodeling of the rat pulmonary artery.Chaosheng DengZhanghua ZhongDawen WuYunfei ChenNingfang LianHaibo DingQiaoxian ZhangQichang LinShuang WuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chaosheng Deng Zhanghua Zhong Dawen Wu Yunfei Chen Ningfang Lian Haibo Ding Qiaoxian Zhang Qichang Lin Shuang Wu Role of FoxO1 and apoptosis in pulmonary vascular remolding in a rat model of chronic thromboembolic pulmonary hypertension
description	Abstract To explore the role of FoxO1 and apoptosis in a rat model of chronic thromboembolic pulmonary hypertension (CTEPH). Rats were randomly divided into a sham group (n = 45) and an experimental group (n = 45). Autologous blood clots were injected into rats three times to induce CTEPH. Rats were further divided into three subgroups: a 1-week subgroup (n = 15), a 2-week subgroup (n = 15), and a 4-week subgroup (n = 15). Mean pulmonary arterial pressure (mPAP) and histopathology were evaluated at each time point. FoxO1, Bad, and Bcl-2 levels were examined at each time point using reverse transcription PCR and western blotting. The mPAP and vessel wall area/total area (WA/TA) ratio in the experimental group gradually increased in a time-dependent manner (P < 0.05). Both the mRNA and protein levels of FoxO1 decreased in the CTEPH rats compared to in the sham group. In addition, embolization led to the up-regulation of Bad and the down-regulation of Bcl-2 (P < 0.05). FoxO1 and apoptosis play an important role in the pathogenesis of CTEPH. Apoptosis-resistant pulmonary artery endothelial cells may play an important role in remodeling of the rat pulmonary artery.
format	article
author	Chaosheng Deng Zhanghua Zhong Dawen Wu Yunfei Chen Ningfang Lian Haibo Ding Qiaoxian Zhang Qichang Lin Shuang Wu
author_facet	Chaosheng Deng Zhanghua Zhong Dawen Wu Yunfei Chen Ningfang Lian Haibo Ding Qiaoxian Zhang Qichang Lin Shuang Wu
author_sort	Chaosheng Deng
title	Role of FoxO1 and apoptosis in pulmonary vascular remolding in a rat model of chronic thromboembolic pulmonary hypertension
title_short	Role of FoxO1 and apoptosis in pulmonary vascular remolding in a rat model of chronic thromboembolic pulmonary hypertension
title_full	Role of FoxO1 and apoptosis in pulmonary vascular remolding in a rat model of chronic thromboembolic pulmonary hypertension
title_fullStr	Role of FoxO1 and apoptosis in pulmonary vascular remolding in a rat model of chronic thromboembolic pulmonary hypertension
title_full_unstemmed	Role of FoxO1 and apoptosis in pulmonary vascular remolding in a rat model of chronic thromboembolic pulmonary hypertension
title_sort	role of foxo1 and apoptosis in pulmonary vascular remolding in a rat model of chronic thromboembolic pulmonary hypertension
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d5d19a3198554d49b1614cbb868a2ac9
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_version_	1718387478122987520

Role of FoxO1 and apoptosis in pulmonary vascular remolding in a rat model of chronic thromboembolic pulmonary hypertension

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