MicroRNA-4521 targets hepatoma up-regulated protein (HURP) to inhibit the malignant progression of breast cancer

Breast cancer (BC) is a common malignancy among women, and microRNAs (miRNAs) play a role in its progression. Reportedly, microRNA-4521 (miR-4521) participates in regulating the progression of some carcinomas. In the present work, miR-4521 and hepatoma up-regulated protein (HURP) mRNA expressions in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Changwen Li, Sen Peng, Chuangang Tang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	mir-4521 breast cancer hurp nf-κb Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/d5e470e1d5e74cb0a8595ffd8048b511
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d5e470e1d5e74cb0a8595ffd8048b511
record_format	dspace
spelling	oai:doaj.org-article:d5e470e1d5e74cb0a8595ffd8048b5112021-11-04T15:51:54ZMicroRNA-4521 targets hepatoma up-regulated protein (HURP) to inhibit the malignant progression of breast cancer2165-59792165-598710.1080/21655979.2021.1996016https://doaj.org/article/d5e470e1d5e74cb0a8595ffd8048b5112021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1996016https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Breast cancer (BC) is a common malignancy among women, and microRNAs (miRNAs) play a role in its progression. Reportedly, microRNA-4521 (miR-4521) participates in regulating the progression of some carcinomas. In the present work, miR-4521 and hepatoma up-regulated protein (HURP) mRNA expressions in BC tissues and cell lines were examined by qRT-PCR. After miR-4521 was overexpressed or knocked down in BC cells, CCK-8 experiment and EdU experiment were employed to detect BC cell growth. Moreover, the Transwell assay was adopted to detect the migration and invasion. Subsequently, flow cytometry was executed to evaluate the changes in cell cycle progression. KEGG analysis was utilized to predict the signaling pathways related with the target genes of miR-524-5p. The binding relationship between miR-4521 and HURP 3ʹUTR was validated by dual-luciferase reporter gene experiment. HURP and NF-kB p65 protein expression in BC cells was detected by Western blot. It was revealed that, miR-4521 was lowly expressed in BC tissues, and its expression was associated with tumor grade, lymph node metastasis and clinical stage of the patients. Overexpression of miR-4521 suppressed the growth, migration, invasion and cell cycle progression of BC cells and restrained p65 expression; downregulation of miR-4521 in BC cells showed opposite biological effects. Additionally, HURP was a downstream target gene of miR-4521, and overexpression of HURP reversed the above effects of miR-4521 overexpression. In short, miR-4521 can inhibit BC progression by modulating the HURP/NF-κB pathway.Changwen LiSen PengChuangang TangTaylor & Francis Grouparticlemir-4521breast cancerhurpnf-κbBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mir-4521 breast cancer hurp nf-κb Biotechnology TP248.13-248.65
spellingShingle	mir-4521 breast cancer hurp nf-κb Biotechnology TP248.13-248.65 Changwen Li Sen Peng Chuangang Tang MicroRNA-4521 targets hepatoma up-regulated protein (HURP) to inhibit the malignant progression of breast cancer
description	Breast cancer (BC) is a common malignancy among women, and microRNAs (miRNAs) play a role in its progression. Reportedly, microRNA-4521 (miR-4521) participates in regulating the progression of some carcinomas. In the present work, miR-4521 and hepatoma up-regulated protein (HURP) mRNA expressions in BC tissues and cell lines were examined by qRT-PCR. After miR-4521 was overexpressed or knocked down in BC cells, CCK-8 experiment and EdU experiment were employed to detect BC cell growth. Moreover, the Transwell assay was adopted to detect the migration and invasion. Subsequently, flow cytometry was executed to evaluate the changes in cell cycle progression. KEGG analysis was utilized to predict the signaling pathways related with the target genes of miR-524-5p. The binding relationship between miR-4521 and HURP 3ʹUTR was validated by dual-luciferase reporter gene experiment. HURP and NF-kB p65 protein expression in BC cells was detected by Western blot. It was revealed that, miR-4521 was lowly expressed in BC tissues, and its expression was associated with tumor grade, lymph node metastasis and clinical stage of the patients. Overexpression of miR-4521 suppressed the growth, migration, invasion and cell cycle progression of BC cells and restrained p65 expression; downregulation of miR-4521 in BC cells showed opposite biological effects. Additionally, HURP was a downstream target gene of miR-4521, and overexpression of HURP reversed the above effects of miR-4521 overexpression. In short, miR-4521 can inhibit BC progression by modulating the HURP/NF-κB pathway.
format	article
author	Changwen Li Sen Peng Chuangang Tang
author_facet	Changwen Li Sen Peng Chuangang Tang
author_sort	Changwen Li
title	MicroRNA-4521 targets hepatoma up-regulated protein (HURP) to inhibit the malignant progression of breast cancer
title_short	MicroRNA-4521 targets hepatoma up-regulated protein (HURP) to inhibit the malignant progression of breast cancer
title_full	MicroRNA-4521 targets hepatoma up-regulated protein (HURP) to inhibit the malignant progression of breast cancer
title_fullStr	MicroRNA-4521 targets hepatoma up-regulated protein (HURP) to inhibit the malignant progression of breast cancer
title_full_unstemmed	MicroRNA-4521 targets hepatoma up-regulated protein (HURP) to inhibit the malignant progression of breast cancer
title_sort	microrna-4521 targets hepatoma up-regulated protein (hurp) to inhibit the malignant progression of breast cancer
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/d5e470e1d5e74cb0a8595ffd8048b511
work_keys_str_mv	AT changwenli microrna4521targetshepatomaupregulatedproteinhurptoinhibitthemalignantprogressionofbreastcancer AT senpeng microrna4521targetshepatomaupregulatedproteinhurptoinhibitthemalignantprogressionofbreastcancer AT chuangangtang microrna4521targetshepatomaupregulatedproteinhurptoinhibitthemalignantprogressionofbreastcancer
_version_	1718444742553894912

MicroRNA-4521 targets hepatoma up-regulated protein (HURP) to inhibit the malignant progression of breast cancer

Ejemplares similares