Expression and function of the protein tyrosine phosphatase receptor J (PTPRJ) in normal mammary epithelial cells and breast tumors.

The protein tyrosine phosphatase receptor J, PTPRJ, is a tumor suppressor gene that has been implicated in a range of cancers, including breast cancer, yet little is known about its role in normal breast physiology or in mammary gland tumorigenesis. In this paper we show that PTPRJ mRNA is expressed...

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Autores principales: Chanel E Smart, Marjan E Askarian Amiri, Ania Wronski, Marcel E Dinger, Joanna Crawford, Dmitry A Ovchinnikov, Ana Cristina Vargas, Lynne Reid, Peter T Simpson, Sarah Song, Christiane Wiesner, Juliet D French, Richa K Dave, Leonard da Silva, Amy Purdon, Megan Andrew, John S Mattick, Sunil R Lakhani, Melissa A Brown, Stuart Kellie
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Publicado: Public Library of Science (PLoS) 2012
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spelling oai:doaj.org-article:d5eaa5f72a81446caa82d85295dc89092021-11-18T07:12:15ZExpression and function of the protein tyrosine phosphatase receptor J (PTPRJ) in normal mammary epithelial cells and breast tumors.1932-620310.1371/journal.pone.0040742https://doaj.org/article/d5eaa5f72a81446caa82d85295dc89092012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22815804/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The protein tyrosine phosphatase receptor J, PTPRJ, is a tumor suppressor gene that has been implicated in a range of cancers, including breast cancer, yet little is known about its role in normal breast physiology or in mammary gland tumorigenesis. In this paper we show that PTPRJ mRNA is expressed in normal breast tissue and reduced in corresponding tumors. Meta-analysis revealed that the gene encoding PTPRJ is frequently lost in breast tumors and that low expression of the transcript associated with poorer overall survival at 20 years. Immunohistochemistry of PTPRJ protein in normal human breast tissue revealed a distinctive apical localisation in the luminal cells of alveoli and ducts. Qualitative analysis of a cohort of invasive ductal carcinomas revealed retention of normal apical PTPRJ localization where tubule formation was maintained but that tumors mostly exhibited diffuse cytoplasmic staining, indicating that dysregulation of localisation associated with loss of tissue architecture in tumorigenesis. The murine ortholog, Ptprj, exhibited a similar localisation in normal mammary gland, and was differentially regulated throughout lactational development, and in an in vitro model of mammary epithelial differentiation. Furthermore, ectopic expression of human PTPRJ in HC11 murine mammary epithelial cells inhibited dome formation. These data indicate that PTPRJ may regulate differentiation of normal mammary epithelia and that dysregulation of protein localisation may be associated with tumorigenesis.Chanel E SmartMarjan E Askarian AmiriAnia WronskiMarcel E DingerJoanna CrawfordDmitry A OvchinnikovAna Cristina VargasLynne ReidPeter T SimpsonSarah SongChristiane WiesnerJuliet D FrenchRicha K DaveLeonard da SilvaAmy PurdonMegan AndrewJohn S MattickSunil R LakhaniMelissa A BrownStuart KelliePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e40742 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Chanel E Smart
Marjan E Askarian Amiri
Ania Wronski
Marcel E Dinger
Joanna Crawford
Dmitry A Ovchinnikov
Ana Cristina Vargas
Lynne Reid
Peter T Simpson
Sarah Song
Christiane Wiesner
Juliet D French
Richa K Dave
Leonard da Silva
Amy Purdon
Megan Andrew
John S Mattick
Sunil R Lakhani
Melissa A Brown
Stuart Kellie
Expression and function of the protein tyrosine phosphatase receptor J (PTPRJ) in normal mammary epithelial cells and breast tumors.
description The protein tyrosine phosphatase receptor J, PTPRJ, is a tumor suppressor gene that has been implicated in a range of cancers, including breast cancer, yet little is known about its role in normal breast physiology or in mammary gland tumorigenesis. In this paper we show that PTPRJ mRNA is expressed in normal breast tissue and reduced in corresponding tumors. Meta-analysis revealed that the gene encoding PTPRJ is frequently lost in breast tumors and that low expression of the transcript associated with poorer overall survival at 20 years. Immunohistochemistry of PTPRJ protein in normal human breast tissue revealed a distinctive apical localisation in the luminal cells of alveoli and ducts. Qualitative analysis of a cohort of invasive ductal carcinomas revealed retention of normal apical PTPRJ localization where tubule formation was maintained but that tumors mostly exhibited diffuse cytoplasmic staining, indicating that dysregulation of localisation associated with loss of tissue architecture in tumorigenesis. The murine ortholog, Ptprj, exhibited a similar localisation in normal mammary gland, and was differentially regulated throughout lactational development, and in an in vitro model of mammary epithelial differentiation. Furthermore, ectopic expression of human PTPRJ in HC11 murine mammary epithelial cells inhibited dome formation. These data indicate that PTPRJ may regulate differentiation of normal mammary epithelia and that dysregulation of protein localisation may be associated with tumorigenesis.
format article
author Chanel E Smart
Marjan E Askarian Amiri
Ania Wronski
Marcel E Dinger
Joanna Crawford
Dmitry A Ovchinnikov
Ana Cristina Vargas
Lynne Reid
Peter T Simpson
Sarah Song
Christiane Wiesner
Juliet D French
Richa K Dave
Leonard da Silva
Amy Purdon
Megan Andrew
John S Mattick
Sunil R Lakhani
Melissa A Brown
Stuart Kellie
author_facet Chanel E Smart
Marjan E Askarian Amiri
Ania Wronski
Marcel E Dinger
Joanna Crawford
Dmitry A Ovchinnikov
Ana Cristina Vargas
Lynne Reid
Peter T Simpson
Sarah Song
Christiane Wiesner
Juliet D French
Richa K Dave
Leonard da Silva
Amy Purdon
Megan Andrew
John S Mattick
Sunil R Lakhani
Melissa A Brown
Stuart Kellie
author_sort Chanel E Smart
title Expression and function of the protein tyrosine phosphatase receptor J (PTPRJ) in normal mammary epithelial cells and breast tumors.
title_short Expression and function of the protein tyrosine phosphatase receptor J (PTPRJ) in normal mammary epithelial cells and breast tumors.
title_full Expression and function of the protein tyrosine phosphatase receptor J (PTPRJ) in normal mammary epithelial cells and breast tumors.
title_fullStr Expression and function of the protein tyrosine phosphatase receptor J (PTPRJ) in normal mammary epithelial cells and breast tumors.
title_full_unstemmed Expression and function of the protein tyrosine phosphatase receptor J (PTPRJ) in normal mammary epithelial cells and breast tumors.
title_sort expression and function of the protein tyrosine phosphatase receptor j (ptprj) in normal mammary epithelial cells and breast tumors.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/d5eaa5f72a81446caa82d85295dc8909
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