Quantum dots-based lateral flow immunoassay combined with image analysis for semiquantitative detection of IgE antibody to mite

Yan Zhao,1,* Qiang Zhang,2,* Qingfeng Meng,3 Fenglian Wu,4 Lihua Zhang,1 Yao Tang,1 Yuanyuan Guan,1 Lixin An1 1Department of Allergy, The First Affiliated Hospital, Harbin Medical University, Harbin, 2Department of Otorhinolaryngology Head and Neck Surgery, The Affiliated Yantai Yuhuangding Hospita...

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Autores principales:	Zhao Y, Zhang Q, Meng Q, Wu F, Zhang L, Tang Y, Guan Y, An L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Lateral flow immunoassay Quantum dots House dust mite IgE Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d5ee6a2d07ba4aefb8592283fa3bf52f
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spelling	oai:doaj.org-article:d5ee6a2d07ba4aefb8592283fa3bf52f2021-12-02T06:46:22ZQuantum dots-based lateral flow immunoassay combined with image analysis for semiquantitative detection of IgE antibody to mite1178-2013https://doaj.org/article/d5ee6a2d07ba4aefb8592283fa3bf52f2017-07-01T00:00:00Zhttps://www.dovepress.com/quantum-dots-based-lateral-flow-immunoassay-combined-with-image-analys-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yan Zhao,1,* Qiang Zhang,2,* Qingfeng Meng,3 Fenglian Wu,4 Lihua Zhang,1 Yao Tang,1 Yuanyuan Guan,1 Lixin An1 1Department of Allergy, The First Affiliated Hospital, Harbin Medical University, Harbin, 2Department of Otorhinolaryngology Head and Neck Surgery, The Affiliated Yantai Yuhuangding Hospital, Qingdao University, Yantai, 3Department of Ophthalmology, The First Affiliated Hospital, Harbin Medical University, Harbin, 4Department of Burn and Plastic Surgery, The First Affiliated Hospital of Qinhuangdao, Qinhuangdao, People’s Republic of China *These authors contributed equally to this work Abstract: Semiquantitative and rapid detection of specific IgE (sIgE) with well clinical relevance to house dust mite (HDM) are promising for prevalence rhinitis and asthma patients due to the increasing air pollution. However, the conventional IgE measurement systems are time-consuming, complicated and require special instruments. Herein, we overcome the above limitations of sIgE to HDM detection system by developing a quantum dot nanobeads-based lateral flow immunoassay and an image analysis procedure. The proposed detection system could semiquantitatively measure the IgE in a linear range of 0.2–10 U/mL. Moreover, there is a well correlation between the developed detection system and the clinical symptoms by a comparison study using 56 positive patients’ sera and 40 healthy control sera. The proposed detection system is simple, robust and easy-to-use and promising for in home test. Keywords: lateral flow immunoassay, quantum dots, house dust mite, IgE Zhao YZhang QMeng QWu FZhang LTang YGuan YAn LDove Medical PressarticleLateral flow immunoassayQuantum dotsHouse dust miteIgEMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 4805-4812 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lateral flow immunoassay Quantum dots House dust mite IgE Medicine (General) R5-920
spellingShingle	Lateral flow immunoassay Quantum dots House dust mite IgE Medicine (General) R5-920 Zhao Y Zhang Q Meng Q Wu F Zhang L Tang Y Guan Y An L Quantum dots-based lateral flow immunoassay combined with image analysis for semiquantitative detection of IgE antibody to mite
description	Yan Zhao,1,* Qiang Zhang,2,* Qingfeng Meng,3 Fenglian Wu,4 Lihua Zhang,1 Yao Tang,1 Yuanyuan Guan,1 Lixin An1 1Department of Allergy, The First Affiliated Hospital, Harbin Medical University, Harbin, 2Department of Otorhinolaryngology Head and Neck Surgery, The Affiliated Yantai Yuhuangding Hospital, Qingdao University, Yantai, 3Department of Ophthalmology, The First Affiliated Hospital, Harbin Medical University, Harbin, 4Department of Burn and Plastic Surgery, The First Affiliated Hospital of Qinhuangdao, Qinhuangdao, People’s Republic of China *These authors contributed equally to this work Abstract: Semiquantitative and rapid detection of specific IgE (sIgE) with well clinical relevance to house dust mite (HDM) are promising for prevalence rhinitis and asthma patients due to the increasing air pollution. However, the conventional IgE measurement systems are time-consuming, complicated and require special instruments. Herein, we overcome the above limitations of sIgE to HDM detection system by developing a quantum dot nanobeads-based lateral flow immunoassay and an image analysis procedure. The proposed detection system could semiquantitatively measure the IgE in a linear range of 0.2–10 U/mL. Moreover, there is a well correlation between the developed detection system and the clinical symptoms by a comparison study using 56 positive patients’ sera and 40 healthy control sera. The proposed detection system is simple, robust and easy-to-use and promising for in home test. Keywords: lateral flow immunoassay, quantum dots, house dust mite, IgE
format	article
author	Zhao Y Zhang Q Meng Q Wu F Zhang L Tang Y Guan Y An L
author_facet	Zhao Y Zhang Q Meng Q Wu F Zhang L Tang Y Guan Y An L
author_sort	Zhao Y
title	Quantum dots-based lateral flow immunoassay combined with image analysis for semiquantitative detection of IgE antibody to mite
title_short	Quantum dots-based lateral flow immunoassay combined with image analysis for semiquantitative detection of IgE antibody to mite
title_full	Quantum dots-based lateral flow immunoassay combined with image analysis for semiquantitative detection of IgE antibody to mite
title_fullStr	Quantum dots-based lateral flow immunoassay combined with image analysis for semiquantitative detection of IgE antibody to mite
title_full_unstemmed	Quantum dots-based lateral flow immunoassay combined with image analysis for semiquantitative detection of IgE antibody to mite
title_sort	quantum dots-based lateral flow immunoassay combined with image analysis for semiquantitative detection of ige antibody to mite
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/d5ee6a2d07ba4aefb8592283fa3bf52f
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Quantum dots-based lateral flow immunoassay combined with image analysis for semiquantitative detection of IgE antibody to mite

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