Detoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken

Sections of chicken gut differ in many aspects, e.g., the passage of digesta (continuous vs. discontinuous), the concentration of oxygen, and the density of colonising microbiota. Using an unbiased LC-MS/MS protocol, we compared protein expression in 18 ileal and 57 caecal tissue samples that origin...

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Autores principales:	Jiri Volf, Jana Rajova, Vladimir Babak, Zuzana Seidlerova, Ivan Rychlik
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hydrogen sulphide wound healing caecum ileum stress response chicken Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/d5f5efb0041b4fa6a6b08cff1a8d5964
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spelling	oai:doaj.org-article:d5f5efb0041b4fa6a6b08cff1a8d59642021-11-25T16:17:22ZDetoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken10.3390/ani111131552076-2615https://doaj.org/article/d5f5efb0041b4fa6a6b08cff1a8d59642021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3155https://doaj.org/toc/2076-2615Sections of chicken gut differ in many aspects, e.g., the passage of digesta (continuous vs. discontinuous), the concentration of oxygen, and the density of colonising microbiota. Using an unbiased LC-MS/MS protocol, we compared protein expression in 18 ileal and 57 caecal tissue samples that originated from 7-day old ISA brown chickens. We found that proteins specific to the ileum were either structural (e.g., 3 actin isoforms, villin, or myosin 1A), or those required for nutrient digestion (e.g., sucrose isomaltase, maltase–glucoamylase, peptidase D) and absorption (e.g., fatty acid-binding protein 2 and 6 or bile acid–CoA:amino acid N-acyltransferase). On the other hand, proteins characteristic of the caecum were involved in sensing and limiting the consequences of oxidative stress (e.g., thioredoxin, peroxiredoxin 6), cell adhesion, and motility associated with wound healing (e.g., fibronectin 1, desmoyokin). These mechanisms are coupled with the activation of mechanisms suppressing the inflammatory response (galectin 1). Rather prominent were also expressions of proteins linked to hydrogen sulphide metabolism in caecum represented by cystathionin beta synthase, selenium-binding protein 1, mercaptopyruvate sulphurtransferase, and thiosulphate sulphurtransferase. Higher mRNA expression of nuclear factor, erythroid 2-like 2, the main oxidative stress transcriptional factor in caecum, further supported our observations.Jiri VolfJana RajovaVladimir BabakZuzana SeidlerovaIvan RychlikMDPI AGarticlehydrogen sulphidewound healingcaecumileumstress responsechickenVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3155, p 3155 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hydrogen sulphide wound healing caecum ileum stress response chicken Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	hydrogen sulphide wound healing caecum ileum stress response chicken Veterinary medicine SF600-1100 Zoology QL1-991 Jiri Volf Jana Rajova Vladimir Babak Zuzana Seidlerova Ivan Rychlik Detoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken
description	Sections of chicken gut differ in many aspects, e.g., the passage of digesta (continuous vs. discontinuous), the concentration of oxygen, and the density of colonising microbiota. Using an unbiased LC-MS/MS protocol, we compared protein expression in 18 ileal and 57 caecal tissue samples that originated from 7-day old ISA brown chickens. We found that proteins specific to the ileum were either structural (e.g., 3 actin isoforms, villin, or myosin 1A), or those required for nutrient digestion (e.g., sucrose isomaltase, maltase–glucoamylase, peptidase D) and absorption (e.g., fatty acid-binding protein 2 and 6 or bile acid–CoA:amino acid N-acyltransferase). On the other hand, proteins characteristic of the caecum were involved in sensing and limiting the consequences of oxidative stress (e.g., thioredoxin, peroxiredoxin 6), cell adhesion, and motility associated with wound healing (e.g., fibronectin 1, desmoyokin). These mechanisms are coupled with the activation of mechanisms suppressing the inflammatory response (galectin 1). Rather prominent were also expressions of proteins linked to hydrogen sulphide metabolism in caecum represented by cystathionin beta synthase, selenium-binding protein 1, mercaptopyruvate sulphurtransferase, and thiosulphate sulphurtransferase. Higher mRNA expression of nuclear factor, erythroid 2-like 2, the main oxidative stress transcriptional factor in caecum, further supported our observations.
format	article
author	Jiri Volf Jana Rajova Vladimir Babak Zuzana Seidlerova Ivan Rychlik
author_facet	Jiri Volf Jana Rajova Vladimir Babak Zuzana Seidlerova Ivan Rychlik
author_sort	Jiri Volf
title	Detoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken
title_short	Detoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken
title_full	Detoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken
title_fullStr	Detoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken
title_full_unstemmed	Detoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken
title_sort	detoxification, hydrogen sulphide metabolism and wound healing are the main functions that differentiate caecum protein expression from ileum of week-old chicken
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d5f5efb0041b4fa6a6b08cff1a8d5964
work_keys_str_mv	AT jirivolf detoxificationhydrogensulphidemetabolismandwoundhealingarethemainfunctionsthatdifferentiatecaecumproteinexpressionfromileumofweekoldchicken AT janarajova detoxificationhydrogensulphidemetabolismandwoundhealingarethemainfunctionsthatdifferentiatecaecumproteinexpressionfromileumofweekoldchicken AT vladimirbabak detoxificationhydrogensulphidemetabolismandwoundhealingarethemainfunctionsthatdifferentiatecaecumproteinexpressionfromileumofweekoldchicken AT zuzanaseidlerova detoxificationhydrogensulphidemetabolismandwoundhealingarethemainfunctionsthatdifferentiatecaecumproteinexpressionfromileumofweekoldchicken AT ivanrychlik detoxificationhydrogensulphidemetabolismandwoundhealingarethemainfunctionsthatdifferentiatecaecumproteinexpressionfromileumofweekoldchicken
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Detoxification, Hydrogen Sulphide Metabolism and Wound Healing Are the Main Functions That Differentiate Caecum Protein Expression from Ileum of Week-Old Chicken

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