Mesenchymal Stem/Stromal Cells Overexpressing CXCR4 Revealed Enhanced Migration: A Lesson Learned from the Pathogenesis of WHIM Syndrome

C-X-C chemokine receptor type 4 (CXCR4), initially recognized as a co-receptor for HIV, contributes to several disorders, including the WHIM (Warts, Hypogammaglobulinemia, Infections, and Myelokathexis) syndrome. CXCR4 binds to its ligand SDF-1 to make an axis involved in the homing property of stem...

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Autores principales:	Hamid Reza Bidkhori, Ahmad Reza Bahrami, Moein Farshchian, Asieh Heirani-tabasi, Mahdi Mirahmadi, Halimeh Hasanzadeh, Naghmeh Ahmadiankia, Reza Faridhosseini, Mahtab Dastpak, Arezoo Gowhari Shabgah, Maryam M. Matin
Formato:	article
Lenguaje:	EN
Publicado:	SAGE Publishing 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/d5f7066f83944eb8b348968bd3545411
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spelling	oai:doaj.org-article:d5f7066f83944eb8b348968bd35454112021-12-02T01:33:49ZMesenchymal Stem/Stromal Cells Overexpressing CXCR4 Revealed Enhanced Migration: A Lesson Learned from the Pathogenesis of WHIM Syndrome1555-389210.1177/09636897211054498https://doaj.org/article/d5f7066f83944eb8b348968bd35454112021-11-01T00:00:00Zhttps://doi.org/10.1177/09636897211054498https://doaj.org/toc/1555-3892C-X-C chemokine receptor type 4 (CXCR4), initially recognized as a co-receptor for HIV, contributes to several disorders, including the WHIM (Warts, Hypogammaglobulinemia, Infections, and Myelokathexis) syndrome. CXCR4 binds to its ligand SDF-1 to make an axis involved in the homing property of stem cells. This study aimed to employ WHIM syndrome pathogenesis as an inspirational approach to reinforce cell therapies. Wild type and WHIM-type variants of the CXCR4 gene were chemically synthesized and cloned in the pCDH-513B-1 lentiviral vector. Molecular cloning of the synthetic genes was confirmed by DNA sequencing, and expression of both types of CXCR4 at the protein level was confirmed by western blotting in HEK293T cells. Human adipose-derived mesenchymal stem cells (Ad-MSCs) were isolated, characterized, and subjected to lentiviral transduction with Wild type and WHIM-type variants of CXCR4 . The presence of copGFP-positive MSCs confirmed the high efficiency of transduction. The migration ability of both groups of transduced cells was then assessed by transwell migration assay in the presence or absence of a CXCR4-blocking agent. Our qRT-PCR results showed overexpression of CXCR4 at mRNA level in both groups of transduced MSCs, and expression of WHIM-type CXCR4 was significantly higher than Wild type CXCR4 ( P <0.05). Our results indicated that the migration of genetically modified MSCs expressing WHIM-type CXCR4 had significantly enhanced towards SDF1 in comparison with Wild type CXCR4 ( P <0.05), while it was reduced after treatment with CXCR4 antagonist. These data suggest that overexpression of WHIM-type CXCR4 could lead to enhanced and sustained expression of CXCR4 on human MSCs, which would increase their homing capability; hence it might be an appropriate strategy to improve the efficiency of cell-based therapies.Hamid Reza BidkhoriAhmad Reza BahramiMoein FarshchianAsieh Heirani-tabasiMahdi MirahmadiHalimeh HasanzadehNaghmeh AhmadiankiaReza FaridhosseiniMahtab DastpakArezoo Gowhari ShabgahMaryam M. MatinSAGE PublishingarticleMedicineRENCell Transplantation, Vol 30 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Hamid Reza Bidkhori Ahmad Reza Bahrami Moein Farshchian Asieh Heirani-tabasi Mahdi Mirahmadi Halimeh Hasanzadeh Naghmeh Ahmadiankia Reza Faridhosseini Mahtab Dastpak Arezoo Gowhari Shabgah Maryam M. Matin Mesenchymal Stem/Stromal Cells Overexpressing CXCR4 Revealed Enhanced Migration: A Lesson Learned from the Pathogenesis of WHIM Syndrome
description	C-X-C chemokine receptor type 4 (CXCR4), initially recognized as a co-receptor for HIV, contributes to several disorders, including the WHIM (Warts, Hypogammaglobulinemia, Infections, and Myelokathexis) syndrome. CXCR4 binds to its ligand SDF-1 to make an axis involved in the homing property of stem cells. This study aimed to employ WHIM syndrome pathogenesis as an inspirational approach to reinforce cell therapies. Wild type and WHIM-type variants of the CXCR4 gene were chemically synthesized and cloned in the pCDH-513B-1 lentiviral vector. Molecular cloning of the synthetic genes was confirmed by DNA sequencing, and expression of both types of CXCR4 at the protein level was confirmed by western blotting in HEK293T cells. Human adipose-derived mesenchymal stem cells (Ad-MSCs) were isolated, characterized, and subjected to lentiviral transduction with Wild type and WHIM-type variants of CXCR4 . The presence of copGFP-positive MSCs confirmed the high efficiency of transduction. The migration ability of both groups of transduced cells was then assessed by transwell migration assay in the presence or absence of a CXCR4-blocking agent. Our qRT-PCR results showed overexpression of CXCR4 at mRNA level in both groups of transduced MSCs, and expression of WHIM-type CXCR4 was significantly higher than Wild type CXCR4 ( P <0.05). Our results indicated that the migration of genetically modified MSCs expressing WHIM-type CXCR4 had significantly enhanced towards SDF1 in comparison with Wild type CXCR4 ( P <0.05), while it was reduced after treatment with CXCR4 antagonist. These data suggest that overexpression of WHIM-type CXCR4 could lead to enhanced and sustained expression of CXCR4 on human MSCs, which would increase their homing capability; hence it might be an appropriate strategy to improve the efficiency of cell-based therapies.
format	article
author	Hamid Reza Bidkhori Ahmad Reza Bahrami Moein Farshchian Asieh Heirani-tabasi Mahdi Mirahmadi Halimeh Hasanzadeh Naghmeh Ahmadiankia Reza Faridhosseini Mahtab Dastpak Arezoo Gowhari Shabgah Maryam M. Matin
author_facet	Hamid Reza Bidkhori Ahmad Reza Bahrami Moein Farshchian Asieh Heirani-tabasi Mahdi Mirahmadi Halimeh Hasanzadeh Naghmeh Ahmadiankia Reza Faridhosseini Mahtab Dastpak Arezoo Gowhari Shabgah Maryam M. Matin
author_sort	Hamid Reza Bidkhori
title	Mesenchymal Stem/Stromal Cells Overexpressing CXCR4 Revealed Enhanced Migration: A Lesson Learned from the Pathogenesis of WHIM Syndrome
title_short	Mesenchymal Stem/Stromal Cells Overexpressing CXCR4 Revealed Enhanced Migration: A Lesson Learned from the Pathogenesis of WHIM Syndrome
title_full	Mesenchymal Stem/Stromal Cells Overexpressing CXCR4 Revealed Enhanced Migration: A Lesson Learned from the Pathogenesis of WHIM Syndrome
title_fullStr	Mesenchymal Stem/Stromal Cells Overexpressing CXCR4 Revealed Enhanced Migration: A Lesson Learned from the Pathogenesis of WHIM Syndrome
title_full_unstemmed	Mesenchymal Stem/Stromal Cells Overexpressing CXCR4 Revealed Enhanced Migration: A Lesson Learned from the Pathogenesis of WHIM Syndrome
title_sort	mesenchymal stem/stromal cells overexpressing cxcr4 revealed enhanced migration: a lesson learned from the pathogenesis of whim syndrome
publisher	SAGE Publishing
publishDate	2021
url	https://doaj.org/article/d5f7066f83944eb8b348968bd3545411
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Mesenchymal Stem/Stromal Cells Overexpressing CXCR4 Revealed Enhanced Migration: A Lesson Learned from the Pathogenesis of WHIM Syndrome

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