Arsenic–silicon priming of rice (Oryza sativa L.) seeds influence mineral nutrient uptake and biochemical responses through modulation of Lsi-1, Lsi-2, Lsi-6 and nutrient transporter genes

Abstract Silicon (Si) has attracted substantial attention because of its beneficial effect on plants during abiotic stress, including stress due to arsenic (As). We here report that priming rice seeds with As and Si together, helped the plant to sustain As stress for longer period. We examined Si in...

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Autores principales:	Ehasanullah Khan, Meetu Gupta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d5fa6162a71f45af82e1c28a52fb897d
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spelling	oai:doaj.org-article:d5fa6162a71f45af82e1c28a52fb897d2021-12-02T15:07:49ZArsenic–silicon priming of rice (Oryza sativa L.) seeds influence mineral nutrient uptake and biochemical responses through modulation of Lsi-1, Lsi-2, Lsi-6 and nutrient transporter genes10.1038/s41598-018-28712-32045-2322https://doaj.org/article/d5fa6162a71f45af82e1c28a52fb897d2018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-28712-3https://doaj.org/toc/2045-2322Abstract Silicon (Si) has attracted substantial attention because of its beneficial effect on plants during abiotic stress, including stress due to arsenic (As). We here report that priming rice seeds with As and Si together, helped the plant to sustain As stress for longer period. We examined Si induced tolerance against As in rice seedlings at short (7 d) and long (15 d) exposure periods under As(III) and Si treatments since their germinating stage. Results showed that the expression of As(III) transporter genes OsLsi1, OsLsi2 and OsLsi6 was more in As(III) + Si treatment as compared to control and Si treatment, but lower than As(III) alone treatments. The gene expression was maximum in shoot and root at 15 d over 7 d under both As(III) and As(III) + Si treatment, which ultimately leads to decreased accumulation of As in the presence of Si. Morphological characters, antioxidant capacity, oxidative stress marker (MDA), stress modulators (cysteine, proline), and enzymes related with ascorbate-glutathione cycle significantly altered during As(III) + Si treatment at both exposure periods. Further, macro and micronutrient contents also improved with Si, and differentially regulated 12 key genes (NR, NiR, AMT, NR, GS, GOGAT, PT, PHT1, PHT2, APase, KAT1 and HAK10) related with NPK transport and utilization. Results highlight that Si priming of seeds along with As(III) influences growth positively of As-stressed rice.Ehasanullah KhanMeetu GuptaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-16 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ehasanullah Khan Meetu Gupta Arsenic–silicon priming of rice (Oryza sativa L.) seeds influence mineral nutrient uptake and biochemical responses through modulation of Lsi-1, Lsi-2, Lsi-6 and nutrient transporter genes
description	Abstract Silicon (Si) has attracted substantial attention because of its beneficial effect on plants during abiotic stress, including stress due to arsenic (As). We here report that priming rice seeds with As and Si together, helped the plant to sustain As stress for longer period. We examined Si induced tolerance against As in rice seedlings at short (7 d) and long (15 d) exposure periods under As(III) and Si treatments since their germinating stage. Results showed that the expression of As(III) transporter genes OsLsi1, OsLsi2 and OsLsi6 was more in As(III) + Si treatment as compared to control and Si treatment, but lower than As(III) alone treatments. The gene expression was maximum in shoot and root at 15 d over 7 d under both As(III) and As(III) + Si treatment, which ultimately leads to decreased accumulation of As in the presence of Si. Morphological characters, antioxidant capacity, oxidative stress marker (MDA), stress modulators (cysteine, proline), and enzymes related with ascorbate-glutathione cycle significantly altered during As(III) + Si treatment at both exposure periods. Further, macro and micronutrient contents also improved with Si, and differentially regulated 12 key genes (NR, NiR, AMT, NR, GS, GOGAT, PT, PHT1, PHT2, APase, KAT1 and HAK10) related with NPK transport and utilization. Results highlight that Si priming of seeds along with As(III) influences growth positively of As-stressed rice.
format	article
author	Ehasanullah Khan Meetu Gupta
author_facet	Ehasanullah Khan Meetu Gupta
author_sort	Ehasanullah Khan
title	Arsenic–silicon priming of rice (Oryza sativa L.) seeds influence mineral nutrient uptake and biochemical responses through modulation of Lsi-1, Lsi-2, Lsi-6 and nutrient transporter genes
title_short	Arsenic–silicon priming of rice (Oryza sativa L.) seeds influence mineral nutrient uptake and biochemical responses through modulation of Lsi-1, Lsi-2, Lsi-6 and nutrient transporter genes
title_full	Arsenic–silicon priming of rice (Oryza sativa L.) seeds influence mineral nutrient uptake and biochemical responses through modulation of Lsi-1, Lsi-2, Lsi-6 and nutrient transporter genes
title_fullStr	Arsenic–silicon priming of rice (Oryza sativa L.) seeds influence mineral nutrient uptake and biochemical responses through modulation of Lsi-1, Lsi-2, Lsi-6 and nutrient transporter genes
title_full_unstemmed	Arsenic–silicon priming of rice (Oryza sativa L.) seeds influence mineral nutrient uptake and biochemical responses through modulation of Lsi-1, Lsi-2, Lsi-6 and nutrient transporter genes
title_sort	arsenic–silicon priming of rice (oryza sativa l.) seeds influence mineral nutrient uptake and biochemical responses through modulation of lsi-1, lsi-2, lsi-6 and nutrient transporter genes
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/d5fa6162a71f45af82e1c28a52fb897d
work_keys_str_mv	AT ehasanullahkhan arsenicsiliconprimingofriceoryzasativalseedsinfluencemineralnutrientuptakeandbiochemicalresponsesthroughmodulationoflsi1lsi2lsi6andnutrienttransportergenes AT meetugupta arsenicsiliconprimingofriceoryzasativalseedsinfluencemineralnutrientuptakeandbiochemicalresponsesthroughmodulationoflsi1lsi2lsi6andnutrienttransportergenes
_version_	1718388419320610816

Arsenic–silicon priming of rice (Oryza sativa L.) seeds influence mineral nutrient uptake and biochemical responses through modulation of Lsi-1, Lsi-2, Lsi-6 and nutrient transporter genes

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