Integrative analysis of the gut microbiome and metabolome in a rat model with stress induced irritable bowel syndrome

Abstract Stress is one of the major causes of irritable bowel syndrome (IBS), which is well-known for perturbing the microbiome and exacerbating IBS-associated symptoms. However, changes in the gut microbiome and metabolome in response to colorectal distention (CRD), combined with restraint stress (...

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Autores principales:	Yue Hu, Fang Chen, Haiyong Ye, Bin Lu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d60306ccb146424eb13a8f0b0194975a
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spelling	oai:doaj.org-article:d60306ccb146424eb13a8f0b0194975a2021-12-02T16:37:37ZIntegrative analysis of the gut microbiome and metabolome in a rat model with stress induced irritable bowel syndrome10.1038/s41598-021-97083-z2045-2322https://doaj.org/article/d60306ccb146424eb13a8f0b0194975a2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97083-zhttps://doaj.org/toc/2045-2322Abstract Stress is one of the major causes of irritable bowel syndrome (IBS), which is well-known for perturbing the microbiome and exacerbating IBS-associated symptoms. However, changes in the gut microbiome and metabolome in response to colorectal distention (CRD), combined with restraint stress (RS) administration, remains unclear. In this study, CRD and RS stress were used to construct an IBS rat model. The 16S rRNA gene sequencing was used to characterize the microbiota in ileocecal contents. UHPLC-QTOF-MS/MS assay was used to characterize the metabolome of gut microbiota. As a result, significant gut microbial dysbiosis was observed in stress-induced IBS rats, with the obvious enrichment of three and depletion of 11 bacterial taxa in IBS rats, when compared with those in the control group (q < 0.05). Meanwhile, distinct changes in the fecal metabolic phenotype of stress-induced IBS rats were also found, including five increased and 19 decreased metabolites. Furthermore, phenylalanine, tyrosine and tryptophan biosynthesis were the main metabolic pathways induced by IBS stress. Moreover, the altered gut microbiota had a strong correlation with the changes in metabolism of stress-induced IBS rats. Prevotella bacteria are correlated with the metabolism of 1-Naphthol and Arg.Thr. In conclusion, the gut microbiome, metabolome and their interaction were altered. This may be critical for the development of stress-induced IBS.Yue HuFang ChenHaiyong YeBin LuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yue Hu Fang Chen Haiyong Ye Bin Lu Integrative analysis of the gut microbiome and metabolome in a rat model with stress induced irritable bowel syndrome
description	Abstract Stress is one of the major causes of irritable bowel syndrome (IBS), which is well-known for perturbing the microbiome and exacerbating IBS-associated symptoms. However, changes in the gut microbiome and metabolome in response to colorectal distention (CRD), combined with restraint stress (RS) administration, remains unclear. In this study, CRD and RS stress were used to construct an IBS rat model. The 16S rRNA gene sequencing was used to characterize the microbiota in ileocecal contents. UHPLC-QTOF-MS/MS assay was used to characterize the metabolome of gut microbiota. As a result, significant gut microbial dysbiosis was observed in stress-induced IBS rats, with the obvious enrichment of three and depletion of 11 bacterial taxa in IBS rats, when compared with those in the control group (q < 0.05). Meanwhile, distinct changes in the fecal metabolic phenotype of stress-induced IBS rats were also found, including five increased and 19 decreased metabolites. Furthermore, phenylalanine, tyrosine and tryptophan biosynthesis were the main metabolic pathways induced by IBS stress. Moreover, the altered gut microbiota had a strong correlation with the changes in metabolism of stress-induced IBS rats. Prevotella bacteria are correlated with the metabolism of 1-Naphthol and Arg.Thr. In conclusion, the gut microbiome, metabolome and their interaction were altered. This may be critical for the development of stress-induced IBS.
format	article
author	Yue Hu Fang Chen Haiyong Ye Bin Lu
author_facet	Yue Hu Fang Chen Haiyong Ye Bin Lu
author_sort	Yue Hu
title	Integrative analysis of the gut microbiome and metabolome in a rat model with stress induced irritable bowel syndrome
title_short	Integrative analysis of the gut microbiome and metabolome in a rat model with stress induced irritable bowel syndrome
title_full	Integrative analysis of the gut microbiome and metabolome in a rat model with stress induced irritable bowel syndrome
title_fullStr	Integrative analysis of the gut microbiome and metabolome in a rat model with stress induced irritable bowel syndrome
title_full_unstemmed	Integrative analysis of the gut microbiome and metabolome in a rat model with stress induced irritable bowel syndrome
title_sort	integrative analysis of the gut microbiome and metabolome in a rat model with stress induced irritable bowel syndrome
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d60306ccb146424eb13a8f0b0194975a
work_keys_str_mv	AT yuehu integrativeanalysisofthegutmicrobiomeandmetabolomeinaratmodelwithstressinducedirritablebowelsyndrome AT fangchen integrativeanalysisofthegutmicrobiomeandmetabolomeinaratmodelwithstressinducedirritablebowelsyndrome AT haiyongye integrativeanalysisofthegutmicrobiomeandmetabolomeinaratmodelwithstressinducedirritablebowelsyndrome AT binlu integrativeanalysisofthegutmicrobiomeandmetabolomeinaratmodelwithstressinducedirritablebowelsyndrome
_version_	1718383660912082944

Integrative analysis of the gut microbiome and metabolome in a rat model with stress induced irritable bowel syndrome

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