HBV HBx-Downregulated lncRNA <i>LINC01010</i> Attenuates Cell Proliferation by Interacting with Vimentin
Hepatitis B virus (HBV) infection is closely related to hepatocellular carcinoma (HCC) development. To investigate the mechanism of HBV causing HCC, we previously analyzed the transcription of the HBV-transgenic cell line HepG2-4D14 and parental HepG2 cells and identified a subset of long noncoding...
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| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/d6045593bd564df5a1fb6ce4834b9354 |
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| Sumario: | Hepatitis B virus (HBV) infection is closely related to hepatocellular carcinoma (HCC) development. To investigate the mechanism of HBV causing HCC, we previously analyzed the transcription of the HBV-transgenic cell line HepG2-4D14 and parental HepG2 cells and identified a subset of long noncoding RNAs (lncRNAs) differentially expressed between them. In this study, we focus on lncRNA <i>LINC01010</i>, as it is significantly downregulated in HepG2-4D14 cells and in liver tissues of HCC patients, and positively correlated with survival. We found that HBV-encoded HBx can reduce the transcription of <i>LINC01010</i>. Functional analysis showed that the overexpression of <i>LINC01010</i> inhibits proliferation, migration and invasion of HepG2 cells while the knockdown of <i>LINC01010</i> promotes these processes. By taking the approach of RNA immunoprecipitation (RIP) and mass spectrometry, we identified that <i>LINC01010</i> can interact with vimentin. Further studies demonstrated that <i>LINC01010</i> negatively affects the vimentin network extension and causes more rapid subunit exchange and lower stability of vimentin filaments. In addition, <i>LINC01010</i> can reduce the amount of insoluble vimentin within cells, which suggests that <i>LINC01010</i> interfers with vimentin polymerization. These data indicate that <i>LINC01010</i> can inhibit the assembly of vimentin filament. Thus, we revealed that HBV HBx-downregulated <i>LINC01010</i>, which suppresses cell proliferation and migration by negatively regulating the formation of vimentin filament. Taken together, <i>LINC01010</i> is a potential tumor suppressor that may restrain HBV-related HCC development. |
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