HBV HBx-Downregulated lncRNA <i>LINC01010</i> Attenuates Cell Proliferation by Interacting with Vimentin

Hepatitis B virus (HBV) infection is closely related to hepatocellular carcinoma (HCC) development. To investigate the mechanism of HBV causing HCC, we previously analyzed the transcription of the HBV-transgenic cell line HepG2-4D14 and parental HepG2 cells and identified a subset of long noncoding...

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Autores principales:	Lipeng Gan, Qilin Shangguan, Fang Zhang, Xiaomei Tong, Dandan Qi, Yan Zhao, Xin Ye
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	HBV <i>LINC01010</i> vimentin cell proliferation Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/d6045593bd564df5a1fb6ce4834b9354
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spelling	oai:doaj.org-article:d6045593bd564df5a1fb6ce4834b93542021-11-25T17:57:15ZHBV HBx-Downregulated lncRNA <i>LINC01010</i> Attenuates Cell Proliferation by Interacting with Vimentin10.3390/ijms2222124971422-00671661-6596https://doaj.org/article/d6045593bd564df5a1fb6ce4834b93542021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12497https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Hepatitis B virus (HBV) infection is closely related to hepatocellular carcinoma (HCC) development. To investigate the mechanism of HBV causing HCC, we previously analyzed the transcription of the HBV-transgenic cell line HepG2-4D14 and parental HepG2 cells and identified a subset of long noncoding RNAs (lncRNAs) differentially expressed between them. In this study, we focus on lncRNA <i>LINC01010</i>, as it is significantly downregulated in HepG2-4D14 cells and in liver tissues of HCC patients, and positively correlated with survival. We found that HBV-encoded HBx can reduce the transcription of <i>LINC01010</i>. Functional analysis showed that the overexpression of <i>LINC01010</i> inhibits proliferation, migration and invasion of HepG2 cells while the knockdown of <i>LINC01010</i> promotes these processes. By taking the approach of RNA immunoprecipitation (RIP) and mass spectrometry, we identified that <i>LINC01010</i> can interact with vimentin. Further studies demonstrated that <i>LINC01010</i> negatively affects the vimentin network extension and causes more rapid subunit exchange and lower stability of vimentin filaments. In addition, <i>LINC01010</i> can reduce the amount of insoluble vimentin within cells, which suggests that <i>LINC01010</i> interfers with vimentin polymerization. These data indicate that <i>LINC01010</i> can inhibit the assembly of vimentin filament. Thus, we revealed that HBV HBx-downregulated <i>LINC01010</i>, which suppresses cell proliferation and migration by negatively regulating the formation of vimentin filament. Taken together, <i>LINC01010</i> is a potential tumor suppressor that may restrain HBV-related HCC development.Lipeng GanQilin ShangguanFang ZhangXiaomei TongDandan QiYan ZhaoXin YeMDPI AGarticleHBV<i>LINC01010</i>vimentincell proliferationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12497, p 12497 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HBV <i>LINC01010</i> vimentin cell proliferation Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	HBV <i>LINC01010</i> vimentin cell proliferation Biology (General) QH301-705.5 Chemistry QD1-999 Lipeng Gan Qilin Shangguan Fang Zhang Xiaomei Tong Dandan Qi Yan Zhao Xin Ye HBV HBx-Downregulated lncRNA <i>LINC01010</i> Attenuates Cell Proliferation by Interacting with Vimentin
description	Hepatitis B virus (HBV) infection is closely related to hepatocellular carcinoma (HCC) development. To investigate the mechanism of HBV causing HCC, we previously analyzed the transcription of the HBV-transgenic cell line HepG2-4D14 and parental HepG2 cells and identified a subset of long noncoding RNAs (lncRNAs) differentially expressed between them. In this study, we focus on lncRNA <i>LINC01010</i>, as it is significantly downregulated in HepG2-4D14 cells and in liver tissues of HCC patients, and positively correlated with survival. We found that HBV-encoded HBx can reduce the transcription of <i>LINC01010</i>. Functional analysis showed that the overexpression of <i>LINC01010</i> inhibits proliferation, migration and invasion of HepG2 cells while the knockdown of <i>LINC01010</i> promotes these processes. By taking the approach of RNA immunoprecipitation (RIP) and mass spectrometry, we identified that <i>LINC01010</i> can interact with vimentin. Further studies demonstrated that <i>LINC01010</i> negatively affects the vimentin network extension and causes more rapid subunit exchange and lower stability of vimentin filaments. In addition, <i>LINC01010</i> can reduce the amount of insoluble vimentin within cells, which suggests that <i>LINC01010</i> interfers with vimentin polymerization. These data indicate that <i>LINC01010</i> can inhibit the assembly of vimentin filament. Thus, we revealed that HBV HBx-downregulated <i>LINC01010</i>, which suppresses cell proliferation and migration by negatively regulating the formation of vimentin filament. Taken together, <i>LINC01010</i> is a potential tumor suppressor that may restrain HBV-related HCC development.
format	article
author	Lipeng Gan Qilin Shangguan Fang Zhang Xiaomei Tong Dandan Qi Yan Zhao Xin Ye
author_facet	Lipeng Gan Qilin Shangguan Fang Zhang Xiaomei Tong Dandan Qi Yan Zhao Xin Ye
author_sort	Lipeng Gan
title	HBV HBx-Downregulated lncRNA <i>LINC01010</i> Attenuates Cell Proliferation by Interacting with Vimentin
title_short	HBV HBx-Downregulated lncRNA <i>LINC01010</i> Attenuates Cell Proliferation by Interacting with Vimentin
title_full	HBV HBx-Downregulated lncRNA <i>LINC01010</i> Attenuates Cell Proliferation by Interacting with Vimentin
title_fullStr	HBV HBx-Downregulated lncRNA <i>LINC01010</i> Attenuates Cell Proliferation by Interacting with Vimentin
title_full_unstemmed	HBV HBx-Downregulated lncRNA <i>LINC01010</i> Attenuates Cell Proliferation by Interacting with Vimentin
title_sort	hbv hbx-downregulated lncrna <i>linc01010</i> attenuates cell proliferation by interacting with vimentin
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d6045593bd564df5a1fb6ce4834b9354
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HBV HBx-Downregulated lncRNA <i>LINC01010</i> Attenuates Cell Proliferation by Interacting with Vimentin

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