Comparative evaluation of antibacterial property of bioactive glass alone and its combination with chlorhexidine against Enterococcus faecalis – An In vitro study

Context: One of the most common organisms responsible for root canal failure even after the usage of intracanal medicament is Enterococcus faecalis. Newer medicaments with higher antibacterial efficacy against E. faecalis need to be analyzed. Aim: The aim of this study was to compare the antibacteri...

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Autores principales:	S Soumya, M A Faisal Gaffoor, Anoop Samuel, Rethi Gopakumar, C Sabari Girish, N C Sajeena
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2021
Materias:	antimicrobial bioactive glass chlorhexidine combination enterococcus faecalis Pharmacy and materia medica RS1-441 Analytical chemistry QD71-142
Acceso en línea:	https://doaj.org/article/d60d47ed78cb42d78ddaa97e22d12cab
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spelling	oai:doaj.org-article:d60d47ed78cb42d78ddaa97e22d12cab2021-11-19T12:15:53ZComparative evaluation of antibacterial property of bioactive glass alone and its combination with chlorhexidine against Enterococcus faecalis – An In vitro study0975-740610.4103/jpbs.jpbs_249_21https://doaj.org/article/d60d47ed78cb42d78ddaa97e22d12cab2021-01-01T00:00:00Zhttp://www.jpbsonline.org/article.asp?issn=0975-7406;year=2021;volume=13;issue=6;spage=1448;epage=1451;aulast=Soumyahttps://doaj.org/toc/0975-7406Context: One of the most common organisms responsible for root canal failure even after the usage of intracanal medicament is Enterococcus faecalis. Newer medicaments with higher antibacterial efficacy against E. faecalis need to be analyzed. Aim: The aim of this study was to compare the antibacterial property of bioactive glass (BAG) alone and its combination with chlorhexidine (CHX) against E. faecalis. Materials and Methods: Eighty permanent maxillary single-rooted teeth were included in the study. These teeth were randomly divided into three groups of ten teeth each. Dentin blocks were prepared. After debris and smear layer removal, dentin blocks were inoculated with E. faecalis and incubated for 7 days for biofilm formation. Medications including BAG alone and combination of CHX with BAG were placed into the dentin blocks and incubated at 37°C for 72 h. Dentinal shavings were collected, and the mean bacterial count was recorded. Statistical Analysis: The comparison of colony-forming unit (CFU) counts was done using one-way analysis of variance, Tukey's post hoc test, and unpaired t-test. The analysis was done using the Statistical Package for the Social Sciences version 20.0 for Windows. Results: Based on the CFU count obtained after 72 h, compared to the control group, both Group 1 and Group 2 reduced the bacterial load effectively. In comparison with Group 1, Group 2 (combination) was found to be more effective against E. faecalis. Conclusion: It can be concluded that although BAG was effective against E. faecalis, its efficacy increased significantly in combination with CHX.S SoumyaM A Faisal GaffoorAnoop SamuelRethi GopakumarC Sabari GirishN C SajeenaWolters Kluwer Medknow Publicationsarticleantimicrobialbioactive glasschlorhexidinecombinationenterococcus faecalisPharmacy and materia medicaRS1-441Analytical chemistryQD71-142ENJournal of Pharmacy and Bioallied Sciences, Vol 13, Iss 6, Pp 1448-1451 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antimicrobial bioactive glass chlorhexidine combination enterococcus faecalis Pharmacy and materia medica RS1-441 Analytical chemistry QD71-142
spellingShingle	antimicrobial bioactive glass chlorhexidine combination enterococcus faecalis Pharmacy and materia medica RS1-441 Analytical chemistry QD71-142 S Soumya M A Faisal Gaffoor Anoop Samuel Rethi Gopakumar C Sabari Girish N C Sajeena Comparative evaluation of antibacterial property of bioactive glass alone and its combination with chlorhexidine against Enterococcus faecalis – An In vitro study
description	Context: One of the most common organisms responsible for root canal failure even after the usage of intracanal medicament is Enterococcus faecalis. Newer medicaments with higher antibacterial efficacy against E. faecalis need to be analyzed. Aim: The aim of this study was to compare the antibacterial property of bioactive glass (BAG) alone and its combination with chlorhexidine (CHX) against E. faecalis. Materials and Methods: Eighty permanent maxillary single-rooted teeth were included in the study. These teeth were randomly divided into three groups of ten teeth each. Dentin blocks were prepared. After debris and smear layer removal, dentin blocks were inoculated with E. faecalis and incubated for 7 days for biofilm formation. Medications including BAG alone and combination of CHX with BAG were placed into the dentin blocks and incubated at 37°C for 72 h. Dentinal shavings were collected, and the mean bacterial count was recorded. Statistical Analysis: The comparison of colony-forming unit (CFU) counts was done using one-way analysis of variance, Tukey's post hoc test, and unpaired t-test. The analysis was done using the Statistical Package for the Social Sciences version 20.0 for Windows. Results: Based on the CFU count obtained after 72 h, compared to the control group, both Group 1 and Group 2 reduced the bacterial load effectively. In comparison with Group 1, Group 2 (combination) was found to be more effective against E. faecalis. Conclusion: It can be concluded that although BAG was effective against E. faecalis, its efficacy increased significantly in combination with CHX.
format	article
author	S Soumya M A Faisal Gaffoor Anoop Samuel Rethi Gopakumar C Sabari Girish N C Sajeena
author_facet	S Soumya M A Faisal Gaffoor Anoop Samuel Rethi Gopakumar C Sabari Girish N C Sajeena
author_sort	S Soumya
title	Comparative evaluation of antibacterial property of bioactive glass alone and its combination with chlorhexidine against Enterococcus faecalis – An In vitro study
title_short	Comparative evaluation of antibacterial property of bioactive glass alone and its combination with chlorhexidine against Enterococcus faecalis – An In vitro study
title_full	Comparative evaluation of antibacterial property of bioactive glass alone and its combination with chlorhexidine against Enterococcus faecalis – An In vitro study
title_fullStr	Comparative evaluation of antibacterial property of bioactive glass alone and its combination with chlorhexidine against Enterococcus faecalis – An In vitro study
title_full_unstemmed	Comparative evaluation of antibacterial property of bioactive glass alone and its combination with chlorhexidine against Enterococcus faecalis – An In vitro study
title_sort	comparative evaluation of antibacterial property of bioactive glass alone and its combination with chlorhexidine against enterococcus faecalis – an in vitro study
publisher	Wolters Kluwer Medknow Publications
publishDate	2021
url	https://doaj.org/article/d60d47ed78cb42d78ddaa97e22d12cab
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Comparative evaluation of antibacterial property of bioactive glass alone and its combination with chlorhexidine against Enterococcus faecalis – An In vitro study

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