The role of SwrA, DegU and P(D3) in fla/che expression in B. subtilis.
In B. subtilis swarming and robust swimming motility require the positive trigger of SwrA on fla/che operon expression. Despite having an essential and specific activity, how SwrA executes this task has remained elusive thus far. We demonstrate here that SwrA acts at the main σ(A)-dependent fla/che...
Guardado en:
| Autores principales: | , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2013
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/d60eb2efe28740e1913e3fad2cec6041 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:d60eb2efe28740e1913e3fad2cec6041 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:d60eb2efe28740e1913e3fad2cec60412021-11-18T08:40:01ZThe role of SwrA, DegU and P(D3) in fla/che expression in B. subtilis.1932-620310.1371/journal.pone.0085065https://doaj.org/article/d60eb2efe28740e1913e3fad2cec60412013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24386445/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In B. subtilis swarming and robust swimming motility require the positive trigger of SwrA on fla/che operon expression. Despite having an essential and specific activity, how SwrA executes this task has remained elusive thus far. We demonstrate here that SwrA acts at the main σ(A)-dependent fla/che promoter PA(fla/che) through DegU. Electrophoretic mobility shift assays (EMSA) reveal that SwrA forms a complex with the phosphorylated form of DegU (DegU~P) at PA(fla/che) while it is unable to do so with either unphosphorylated DegU or the DegU32(Hy) mutant protein. Motility assays show that a highly phosphorylated DegU is not detrimental for flagellar motility provided that SwrA is present; however, DegU~P represses PA(fla/che) in the absence of SwrA. Overall, our data support a model in which DegU~P is a dual regulator, acting either as a repressor when alone or as a positive regulator of PA(fla/che) when combined with SwrA. Finally, we demonstrate that the σ(D)-dependent PD3(fla/che) promoter plays an important role in motility, representing a contingent feedback loop necessary to maintain basal motility when swrA is switched to the non-functional swrA(-) status.Serena MordiniCecilia OseraSimone MariniFrancesco ScavoneRiccardo BellazziAlessandro GalizziCinzia CalvioPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e85065 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Serena Mordini Cecilia Osera Simone Marini Francesco Scavone Riccardo Bellazzi Alessandro Galizzi Cinzia Calvio The role of SwrA, DegU and P(D3) in fla/che expression in B. subtilis. |
| description |
In B. subtilis swarming and robust swimming motility require the positive trigger of SwrA on fla/che operon expression. Despite having an essential and specific activity, how SwrA executes this task has remained elusive thus far. We demonstrate here that SwrA acts at the main σ(A)-dependent fla/che promoter PA(fla/che) through DegU. Electrophoretic mobility shift assays (EMSA) reveal that SwrA forms a complex with the phosphorylated form of DegU (DegU~P) at PA(fla/che) while it is unable to do so with either unphosphorylated DegU or the DegU32(Hy) mutant protein. Motility assays show that a highly phosphorylated DegU is not detrimental for flagellar motility provided that SwrA is present; however, DegU~P represses PA(fla/che) in the absence of SwrA. Overall, our data support a model in which DegU~P is a dual regulator, acting either as a repressor when alone or as a positive regulator of PA(fla/che) when combined with SwrA. Finally, we demonstrate that the σ(D)-dependent PD3(fla/che) promoter plays an important role in motility, representing a contingent feedback loop necessary to maintain basal motility when swrA is switched to the non-functional swrA(-) status. |
| format |
article |
| author |
Serena Mordini Cecilia Osera Simone Marini Francesco Scavone Riccardo Bellazzi Alessandro Galizzi Cinzia Calvio |
| author_facet |
Serena Mordini Cecilia Osera Simone Marini Francesco Scavone Riccardo Bellazzi Alessandro Galizzi Cinzia Calvio |
| author_sort |
Serena Mordini |
| title |
The role of SwrA, DegU and P(D3) in fla/che expression in B. subtilis. |
| title_short |
The role of SwrA, DegU and P(D3) in fla/che expression in B. subtilis. |
| title_full |
The role of SwrA, DegU and P(D3) in fla/che expression in B. subtilis. |
| title_fullStr |
The role of SwrA, DegU and P(D3) in fla/che expression in B. subtilis. |
| title_full_unstemmed |
The role of SwrA, DegU and P(D3) in fla/che expression in B. subtilis. |
| title_sort |
role of swra, degu and p(d3) in fla/che expression in b. subtilis. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/d60eb2efe28740e1913e3fad2cec6041 |
| work_keys_str_mv |
AT serenamordini theroleofswradeguandpd3inflacheexpressioninbsubtilis AT ceciliaosera theroleofswradeguandpd3inflacheexpressioninbsubtilis AT simonemarini theroleofswradeguandpd3inflacheexpressioninbsubtilis AT francescoscavone theroleofswradeguandpd3inflacheexpressioninbsubtilis AT riccardobellazzi theroleofswradeguandpd3inflacheexpressioninbsubtilis AT alessandrogalizzi theroleofswradeguandpd3inflacheexpressioninbsubtilis AT cinziacalvio theroleofswradeguandpd3inflacheexpressioninbsubtilis AT serenamordini roleofswradeguandpd3inflacheexpressioninbsubtilis AT ceciliaosera roleofswradeguandpd3inflacheexpressioninbsubtilis AT simonemarini roleofswradeguandpd3inflacheexpressioninbsubtilis AT francescoscavone roleofswradeguandpd3inflacheexpressioninbsubtilis AT riccardobellazzi roleofswradeguandpd3inflacheexpressioninbsubtilis AT alessandrogalizzi roleofswradeguandpd3inflacheexpressioninbsubtilis AT cinziacalvio roleofswradeguandpd3inflacheexpressioninbsubtilis |
| _version_ |
1718421529352470528 |