Effect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from <i>Halomonas desertis</i> G11: Biochemical and Genomic Insights

In this work, a native exopolysaccharide (nEPS) produced by <i>Halomonas desertis</i> G11 isolated from a Tunisian extreme environment was modified by gamma irradiation. Characterization as well as the antioxidant and antitumor activities of nEPS and its gamma-irradiated derivatives (iEP...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Habib Chouchane, Sahar Boutiti, Awatef Ouertani, Wafa Hassen, Sihem Guesmi, Mohamed Neifar, Haikel Jelassi, Haïtham Sghaier, Ahmed Salah Eddine Masmoudi, Ameur Cherif
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antioxidant activities antitumor activities gamma-irradiated derivatives genomic analyses <i>Halomonas desertis</i> G11 native exopolysaccharide Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/d6142512d59b4e19bbc40927c6aff36a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d6142512d59b4e19bbc40927c6aff36a
record_format	dspace
spelling	oai:doaj.org-article:d6142512d59b4e19bbc40927c6aff36a2021-11-11T18:47:40ZEffect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from <i>Halomonas desertis</i> G11: Biochemical and Genomic Insights10.3390/polym132137982073-4360https://doaj.org/article/d6142512d59b4e19bbc40927c6aff36a2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/21/3798https://doaj.org/toc/2073-4360In this work, a native exopolysaccharide (nEPS) produced by <i>Halomonas desertis</i> G11 isolated from a Tunisian extreme environment was modified by gamma irradiation. Characterization as well as the antioxidant and antitumor activities of nEPS and its gamma-irradiated derivatives (iEPSs) were comparatively evaluated. In vitro and in vivo antioxidant potentials were determined by using different methods and through different antioxidant enzymes. The antitumor activity was checked against a human colon cancer cell line. Analyses of the complete genome sequence were carried out to identify genes implicated in the production of nEPS. Thus, the genomic biosynthesis pathway and the export mechanism of nEPS were proposed. Analyses of irradiation data showed that iEPSs acquired new functional groups, lower molecular weights, and gained significantly (<i>p</i> < 0.05) higher antioxidant and antitumor abilities compared with nEPS. These findings provide a basis for using iEPSs as novel pharmaceutical agents for human therapies.Habib ChouchaneSahar BoutitiAwatef OuertaniWafa HassenSihem GuesmiMohamed NeifarHaikel JelassiHaïtham SghaierAhmed Salah Eddine MasmoudiAmeur CherifMDPI AGarticleantioxidant activitiesantitumor activitiesgamma-irradiated derivativesgenomic analyses<i>Halomonas desertis</i> G11native exopolysaccharideOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3798, p 3798 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antioxidant activities antitumor activities gamma-irradiated derivatives genomic analyses <i>Halomonas desertis</i> G11 native exopolysaccharide Organic chemistry QD241-441
spellingShingle	antioxidant activities antitumor activities gamma-irradiated derivatives genomic analyses <i>Halomonas desertis</i> G11 native exopolysaccharide Organic chemistry QD241-441 Habib Chouchane Sahar Boutiti Awatef Ouertani Wafa Hassen Sihem Guesmi Mohamed Neifar Haikel Jelassi Haïtham Sghaier Ahmed Salah Eddine Masmoudi Ameur Cherif Effect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from <i>Halomonas desertis</i> G11: Biochemical and Genomic Insights
description	In this work, a native exopolysaccharide (nEPS) produced by <i>Halomonas desertis</i> G11 isolated from a Tunisian extreme environment was modified by gamma irradiation. Characterization as well as the antioxidant and antitumor activities of nEPS and its gamma-irradiated derivatives (iEPSs) were comparatively evaluated. In vitro and in vivo antioxidant potentials were determined by using different methods and through different antioxidant enzymes. The antitumor activity was checked against a human colon cancer cell line. Analyses of the complete genome sequence were carried out to identify genes implicated in the production of nEPS. Thus, the genomic biosynthesis pathway and the export mechanism of nEPS were proposed. Analyses of irradiation data showed that iEPSs acquired new functional groups, lower molecular weights, and gained significantly (<i>p</i> < 0.05) higher antioxidant and antitumor abilities compared with nEPS. These findings provide a basis for using iEPSs as novel pharmaceutical agents for human therapies.
format	article
author	Habib Chouchane Sahar Boutiti Awatef Ouertani Wafa Hassen Sihem Guesmi Mohamed Neifar Haikel Jelassi Haïtham Sghaier Ahmed Salah Eddine Masmoudi Ameur Cherif
author_facet	Habib Chouchane Sahar Boutiti Awatef Ouertani Wafa Hassen Sihem Guesmi Mohamed Neifar Haikel Jelassi Haïtham Sghaier Ahmed Salah Eddine Masmoudi Ameur Cherif
author_sort	Habib Chouchane
title	Effect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from <i>Halomonas desertis</i> G11: Biochemical and Genomic Insights
title_short	Effect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from <i>Halomonas desertis</i> G11: Biochemical and Genomic Insights
title_full	Effect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from <i>Halomonas desertis</i> G11: Biochemical and Genomic Insights
title_fullStr	Effect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from <i>Halomonas desertis</i> G11: Biochemical and Genomic Insights
title_full_unstemmed	Effect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from <i>Halomonas desertis</i> G11: Biochemical and Genomic Insights
title_sort	effect of gamma irradiation on enhanced biological activities of exopolysaccharide from <i>halomonas desertis</i> g11: biochemical and genomic insights
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d6142512d59b4e19bbc40927c6aff36a
work_keys_str_mv	AT habibchouchane effectofgammairradiationonenhancedbiologicalactivitiesofexopolysaccharidefromihalomonasdesertisig11biochemicalandgenomicinsights AT saharboutiti effectofgammairradiationonenhancedbiologicalactivitiesofexopolysaccharidefromihalomonasdesertisig11biochemicalandgenomicinsights AT awatefouertani effectofgammairradiationonenhancedbiologicalactivitiesofexopolysaccharidefromihalomonasdesertisig11biochemicalandgenomicinsights AT wafahassen effectofgammairradiationonenhancedbiologicalactivitiesofexopolysaccharidefromihalomonasdesertisig11biochemicalandgenomicinsights AT sihemguesmi effectofgammairradiationonenhancedbiologicalactivitiesofexopolysaccharidefromihalomonasdesertisig11biochemicalandgenomicinsights AT mohamedneifar effectofgammairradiationonenhancedbiologicalactivitiesofexopolysaccharidefromihalomonasdesertisig11biochemicalandgenomicinsights AT haikeljelassi effectofgammairradiationonenhancedbiologicalactivitiesofexopolysaccharidefromihalomonasdesertisig11biochemicalandgenomicinsights AT haithamsghaier effectofgammairradiationonenhancedbiologicalactivitiesofexopolysaccharidefromihalomonasdesertisig11biochemicalandgenomicinsights AT ahmedsalaheddinemasmoudi effectofgammairradiationonenhancedbiologicalactivitiesofexopolysaccharidefromihalomonasdesertisig11biochemicalandgenomicinsights AT ameurcherif effectofgammairradiationonenhancedbiologicalactivitiesofexopolysaccharidefromihalomonasdesertisig11biochemicalandgenomicinsights
_version_	1718431731462176768

Effect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from <i>Halomonas desertis</i> G11: Biochemical and Genomic Insights

Ejemplares similares