Single-Cell Approaches to Deconvolute the Development of HSCs

Hematopoietic stem cells (HSCs) play a core role in blood development. The ability to efficiently produce HSCs from various pluripotent stem cell sources is the Holy Grail in the hematology field. However, in vitro or in vivo HSC production remains low, which may be attributable to the lack of under...

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Autores principales:	Yang Xiang, Ryohichi Sugimura
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	HSC single-cell technology molecular barcode spatial transcriptomics Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d615fdb7892b4dcfaa5df8e2d0f8f484
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spelling	oai:doaj.org-article:d615fdb7892b4dcfaa5df8e2d0f8f4842021-11-25T17:08:25ZSingle-Cell Approaches to Deconvolute the Development of HSCs10.3390/cells101128762073-4409https://doaj.org/article/d615fdb7892b4dcfaa5df8e2d0f8f4842021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2876https://doaj.org/toc/2073-4409Hematopoietic stem cells (HSCs) play a core role in blood development. The ability to efficiently produce HSCs from various pluripotent stem cell sources is the Holy Grail in the hematology field. However, in vitro or in vivo HSC production remains low, which may be attributable to the lack of understanding of hematopoiesis. Here, we review the recent progress in this area and introduce advanced technologies, such as single-cell RNA-seq, spatial transcriptomics, and molecular barcoding, which may help to acquire missing information about HSC generation. We finally discuss unresolved questions, the answers to which may be conducive to HSC production, providing a promising path toward HSC-based immunotherapies.Yang XiangRyohichi SugimuraMDPI AGarticleHSCsingle-cell technologymolecular barcodespatial transcriptomicsBiology (General)QH301-705.5ENCells, Vol 10, Iss 2876, p 2876 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HSC single-cell technology molecular barcode spatial transcriptomics Biology (General) QH301-705.5
spellingShingle	HSC single-cell technology molecular barcode spatial transcriptomics Biology (General) QH301-705.5 Yang Xiang Ryohichi Sugimura Single-Cell Approaches to Deconvolute the Development of HSCs
description	Hematopoietic stem cells (HSCs) play a core role in blood development. The ability to efficiently produce HSCs from various pluripotent stem cell sources is the Holy Grail in the hematology field. However, in vitro or in vivo HSC production remains low, which may be attributable to the lack of understanding of hematopoiesis. Here, we review the recent progress in this area and introduce advanced technologies, such as single-cell RNA-seq, spatial transcriptomics, and molecular barcoding, which may help to acquire missing information about HSC generation. We finally discuss unresolved questions, the answers to which may be conducive to HSC production, providing a promising path toward HSC-based immunotherapies.
format	article
author	Yang Xiang Ryohichi Sugimura
author_facet	Yang Xiang Ryohichi Sugimura
author_sort	Yang Xiang
title	Single-Cell Approaches to Deconvolute the Development of HSCs
title_short	Single-Cell Approaches to Deconvolute the Development of HSCs
title_full	Single-Cell Approaches to Deconvolute the Development of HSCs
title_fullStr	Single-Cell Approaches to Deconvolute the Development of HSCs
title_full_unstemmed	Single-Cell Approaches to Deconvolute the Development of HSCs
title_sort	single-cell approaches to deconvolute the development of hscs
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d615fdb7892b4dcfaa5df8e2d0f8f484
work_keys_str_mv	AT yangxiang singlecellapproachestodeconvolutethedevelopmentofhscs AT ryohichisugimura singlecellapproachestodeconvolutethedevelopmentofhscs
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Single-Cell Approaches to Deconvolute the Development of HSCs

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