Nerve growth factor signalling in pathology and regeneration of human teeth

Abstract Nerve growth factor (NGF) is a key regulator of the development and differentiation of neuronal and non-neuronal cells. In the present study we examined the distribution of NGF and its low and high-affinity receptors, p75NTR and TrkA respectively, in permanent human teeth under normal and p...

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Autores principales:	Thimios A. Mitsiadis, Henry Magloire, Pierfrancesco Pagella
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d617576779d349e08e7809a0c032b151
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spelling	oai:doaj.org-article:d617576779d349e08e7809a0c032b1512021-12-02T15:05:32ZNerve growth factor signalling in pathology and regeneration of human teeth10.1038/s41598-017-01455-32045-2322https://doaj.org/article/d617576779d349e08e7809a0c032b1512017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01455-3https://doaj.org/toc/2045-2322Abstract Nerve growth factor (NGF) is a key regulator of the development and differentiation of neuronal and non-neuronal cells. In the present study we examined the distribution of NGF and its low and high-affinity receptors, p75NTR and TrkA respectively, in permanent human teeth under normal and pathological conditions. In intact functional teeth, NGF, p75NTR and TrkA are weakly expressed in dental pulp fibroblasts and odontoblasts that are responsible for dentine formation, while the NGF and p75NTR molecules are strongly expressed in nerve fibres innervating the dental pulp. In carious and injured teeth NGF and TrkA expression is upregulated in a selective manner in odontoblasts surrounding the injury sites, indicating a link between NGF signalling and dental tissue repair events. Accordingly, NGF and TrkA expression is strongly upregulated in cultured primary human dental mesenchymal cells during their differentiation into odontoblasts. Targeted release of NGF in cultured human tooth slices induced extensive axonal growth and migration of Schwann cells towards the NGF administration site. These results show that NGF signalling is strongly linked to pathological and regenerative processes in human teeth and suggest a potential role for this neurotrophic molecule in pulp regeneration.Thimios A. MitsiadisHenry MagloirePierfrancesco PagellaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thimios A. Mitsiadis Henry Magloire Pierfrancesco Pagella Nerve growth factor signalling in pathology and regeneration of human teeth
description	Abstract Nerve growth factor (NGF) is a key regulator of the development and differentiation of neuronal and non-neuronal cells. In the present study we examined the distribution of NGF and its low and high-affinity receptors, p75NTR and TrkA respectively, in permanent human teeth under normal and pathological conditions. In intact functional teeth, NGF, p75NTR and TrkA are weakly expressed in dental pulp fibroblasts and odontoblasts that are responsible for dentine formation, while the NGF and p75NTR molecules are strongly expressed in nerve fibres innervating the dental pulp. In carious and injured teeth NGF and TrkA expression is upregulated in a selective manner in odontoblasts surrounding the injury sites, indicating a link between NGF signalling and dental tissue repair events. Accordingly, NGF and TrkA expression is strongly upregulated in cultured primary human dental mesenchymal cells during their differentiation into odontoblasts. Targeted release of NGF in cultured human tooth slices induced extensive axonal growth and migration of Schwann cells towards the NGF administration site. These results show that NGF signalling is strongly linked to pathological and regenerative processes in human teeth and suggest a potential role for this neurotrophic molecule in pulp regeneration.
format	article
author	Thimios A. Mitsiadis Henry Magloire Pierfrancesco Pagella
author_facet	Thimios A. Mitsiadis Henry Magloire Pierfrancesco Pagella
author_sort	Thimios A. Mitsiadis
title	Nerve growth factor signalling in pathology and regeneration of human teeth
title_short	Nerve growth factor signalling in pathology and regeneration of human teeth
title_full	Nerve growth factor signalling in pathology and regeneration of human teeth
title_fullStr	Nerve growth factor signalling in pathology and regeneration of human teeth
title_full_unstemmed	Nerve growth factor signalling in pathology and regeneration of human teeth
title_sort	nerve growth factor signalling in pathology and regeneration of human teeth
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d617576779d349e08e7809a0c032b151
work_keys_str_mv	AT thimiosamitsiadis nervegrowthfactorsignallinginpathologyandregenerationofhumanteeth AT henrymagloire nervegrowthfactorsignallinginpathologyandregenerationofhumanteeth AT pierfrancescopagella nervegrowthfactorsignallinginpathologyandregenerationofhumanteeth
_version_	1718388796479766528

Nerve growth factor signalling in pathology and regeneration of human teeth

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