Lateral Flow Immunoassay for Visible Detection of Human Brucellosis Based on Blue Silica Nanoparticles
Brucellosis is a highly contagious zoonosis chronic infectious disease with a strong latent capability to endanger human health and economic development via direct or indirect ways. However, the existing methods for brucellosis diagnosis are time-consuming and expensive as they require a tedious exp...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:d6204622354a4f0db05ad698098691162021-12-03T04:44:30ZLateral Flow Immunoassay for Visible Detection of Human Brucellosis Based on Blue Silica Nanoparticles2297-176910.3389/fvets.2021.771341https://doaj.org/article/d6204622354a4f0db05ad698098691162021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.771341/fullhttps://doaj.org/toc/2297-1769Brucellosis is a highly contagious zoonosis chronic infectious disease with a strong latent capability to endanger human health and economic development via direct or indirect ways. However, the existing methods for brucellosis diagnosis are time-consuming and expensive as they require a tedious experimental procedure and a sophisticated experimental device and performance. To overcome these defects, it is truly necessary to establish a real-time, on-site, and rapid detection method for human brucellosis. Here, a lateral flow immunoassay (LFIA) with a rapid, sensitive, and alternative diagnostic procedure for human brucellosis with a high degree of accuracy was developed based on blue silica nanoparticles (SiNPs), Staphylococcal protein A (SPA), and surface Lipopolysaccharide of Brucella spp. (LPS), which can be applied for rapid and feasible detection of human brucellosis. To our knowledge, this is the first report that uses blue SiNPs as a signal probe of LFIA for the rapid diagnosis of human brucellosis. The precursor of blue SiNPs@SPA such as colorless SiNPs and blue SiNPs was synthesized at first and then coupled with SPA onto the surface of blue SiNPs by covalent bond to prepare blue SiNPs@SPA as a capture signal to catch the antibody in the brucellosis-positive serum. When SPA was combined with the antibodies in the brucellosis-positive serum, it was captured by LPS on the test line, forming an antigen–antibody sandwich structure, resulting in the T line turning blue. Finally, the results showed that it is acceptable to use blue SiNPs as visible labels of LFIA, and standard brucellosis serum (containing Brucella spp. antibody at 1,000 IU/ml) could be detected at a dilution of 10−5 and the detection limit of this method was 0.01 IU/ml. Moreover, it also demonstrated good specificity and accuracy for the detection of real human serum samples. Above all, the blue SiNPs-based LFIA that we developed provides a rapid, highly accurate, and inexpensive on-site diagnosis of human brucellosis, and shows great promise in clinical diagnostics for other diseases.Lirui GeLirui GeDan WangDan WangFengnan LianFengnan LianJinbin ZhaoJinbin ZhaoYue WangYue WangYuyi ZhaoYuyi ZhaoLanting ZhangJuan WangXiuling SongJinhua LiKun XuKun XuFrontiers Media S.A.articleblue SiNPsLFIAhuman brucellosisantigen-antibody sandwich structureBrucella spp.Veterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021) |
| institution |
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| collection |
DOAJ |
| language |
EN |
| topic |
blue SiNPs LFIA human brucellosis antigen-antibody sandwich structure Brucella spp. Veterinary medicine SF600-1100 |
| spellingShingle |
blue SiNPs LFIA human brucellosis antigen-antibody sandwich structure Brucella spp. Veterinary medicine SF600-1100 Lirui Ge Lirui Ge Dan Wang Dan Wang Fengnan Lian Fengnan Lian Jinbin Zhao Jinbin Zhao Yue Wang Yue Wang Yuyi Zhao Yuyi Zhao Lanting Zhang Juan Wang Xiuling Song Jinhua Li Kun Xu Kun Xu Lateral Flow Immunoassay for Visible Detection of Human Brucellosis Based on Blue Silica Nanoparticles |
| description |
Brucellosis is a highly contagious zoonosis chronic infectious disease with a strong latent capability to endanger human health and economic development via direct or indirect ways. However, the existing methods for brucellosis diagnosis are time-consuming and expensive as they require a tedious experimental procedure and a sophisticated experimental device and performance. To overcome these defects, it is truly necessary to establish a real-time, on-site, and rapid detection method for human brucellosis. Here, a lateral flow immunoassay (LFIA) with a rapid, sensitive, and alternative diagnostic procedure for human brucellosis with a high degree of accuracy was developed based on blue silica nanoparticles (SiNPs), Staphylococcal protein A (SPA), and surface Lipopolysaccharide of Brucella spp. (LPS), which can be applied for rapid and feasible detection of human brucellosis. To our knowledge, this is the first report that uses blue SiNPs as a signal probe of LFIA for the rapid diagnosis of human brucellosis. The precursor of blue SiNPs@SPA such as colorless SiNPs and blue SiNPs was synthesized at first and then coupled with SPA onto the surface of blue SiNPs by covalent bond to prepare blue SiNPs@SPA as a capture signal to catch the antibody in the brucellosis-positive serum. When SPA was combined with the antibodies in the brucellosis-positive serum, it was captured by LPS on the test line, forming an antigen–antibody sandwich structure, resulting in the T line turning blue. Finally, the results showed that it is acceptable to use blue SiNPs as visible labels of LFIA, and standard brucellosis serum (containing Brucella spp. antibody at 1,000 IU/ml) could be detected at a dilution of 10−5 and the detection limit of this method was 0.01 IU/ml. Moreover, it also demonstrated good specificity and accuracy for the detection of real human serum samples. Above all, the blue SiNPs-based LFIA that we developed provides a rapid, highly accurate, and inexpensive on-site diagnosis of human brucellosis, and shows great promise in clinical diagnostics for other diseases. |
| format |
article |
| author |
Lirui Ge Lirui Ge Dan Wang Dan Wang Fengnan Lian Fengnan Lian Jinbin Zhao Jinbin Zhao Yue Wang Yue Wang Yuyi Zhao Yuyi Zhao Lanting Zhang Juan Wang Xiuling Song Jinhua Li Kun Xu Kun Xu |
| author_facet |
Lirui Ge Lirui Ge Dan Wang Dan Wang Fengnan Lian Fengnan Lian Jinbin Zhao Jinbin Zhao Yue Wang Yue Wang Yuyi Zhao Yuyi Zhao Lanting Zhang Juan Wang Xiuling Song Jinhua Li Kun Xu Kun Xu |
| author_sort |
Lirui Ge |
| title |
Lateral Flow Immunoassay for Visible Detection of Human Brucellosis Based on Blue Silica Nanoparticles |
| title_short |
Lateral Flow Immunoassay for Visible Detection of Human Brucellosis Based on Blue Silica Nanoparticles |
| title_full |
Lateral Flow Immunoassay for Visible Detection of Human Brucellosis Based on Blue Silica Nanoparticles |
| title_fullStr |
Lateral Flow Immunoassay for Visible Detection of Human Brucellosis Based on Blue Silica Nanoparticles |
| title_full_unstemmed |
Lateral Flow Immunoassay for Visible Detection of Human Brucellosis Based on Blue Silica Nanoparticles |
| title_sort |
lateral flow immunoassay for visible detection of human brucellosis based on blue silica nanoparticles |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/d6204622354a4f0db05ad69809869116 |
| work_keys_str_mv |
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| _version_ |
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