Molecular epidemiology of Mycobacterium bovis in Cameroon

Abstract We describe the largest molecular epidemiological study of Bovine Tuberculosis (bTB) in a sub-Saharan African country with higher spatial resolution providing new insights into bTB. Four hundred and ninety-nine samples were collected for culture from 201 and 179 cattle with and without bTB-...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	N. F. Egbe, A. Muwonge, L. Ndip, R. F. Kelly, M. Sander, V. Tanya, V. Ngu Ngwa, I. G. Handel, A. Novak, R. Ngandalo, S. Mazeri, K. L. Morgan, A. Asuquo, B. M. de C. Bronsvoort
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d62a1dd093904985bed3e2041c035f91
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d62a1dd093904985bed3e2041c035f91
record_format	dspace
spelling	oai:doaj.org-article:d62a1dd093904985bed3e2041c035f912021-12-02T16:06:35ZMolecular epidemiology of Mycobacterium bovis in Cameroon10.1038/s41598-017-04230-62045-2322https://doaj.org/article/d62a1dd093904985bed3e2041c035f912017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04230-6https://doaj.org/toc/2045-2322Abstract We describe the largest molecular epidemiological study of Bovine Tuberculosis (bTB) in a sub-Saharan African country with higher spatial resolution providing new insights into bTB. Four hundred and ninety-nine samples were collected for culture from 201 and 179 cattle with and without bTB-like lesions respectively out of 2,346 cattle slaughtered at Bamenda, Ngaoundere, Garoua and Maroua abattoirs between 2012–2013. Two hundred and fifty-five M. bovis were isolated, identified and genotyped using deletion analysis, Hain® Genotype MTBC, spoligotyping and MIRU-VNTR. African 1 was the dominant M. bovis clonal complex, with 97 unique genotypes including 19 novel spoligotypes representing the highest M. bovis genetic diversity observed in Africa to date. SB0944 and SB0953 dominated (63%) the observed spoligotypes. A third of animals with multiple lesions had multiple strain infections. Higher diversity but little evidence of recent transmission of M. bovis was more common in Adamawa compared to the North-West Region. The Adamawa was characterised by a high frequency of singletons possibly due to constant additions from an active livestock movement network compared to the North-West Region where a local expansion was more evident. The latter combined with population-based inferences suggest an unstable and stable bTB-endemic status in the North-West and Adamawa Regions respectively.N. F. EgbeA. MuwongeL. NdipR. F. KellyM. SanderV. TanyaV. Ngu NgwaI. G. HandelA. NovakR. NgandaloS. MazeriK. L. MorganA. AsuquoB. M. de C. BronsvoortNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-17 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q N. F. Egbe A. Muwonge L. Ndip R. F. Kelly M. Sander V. Tanya V. Ngu Ngwa I. G. Handel A. Novak R. Ngandalo S. Mazeri K. L. Morgan A. Asuquo B. M. de C. Bronsvoort Molecular epidemiology of Mycobacterium bovis in Cameroon
description	Abstract We describe the largest molecular epidemiological study of Bovine Tuberculosis (bTB) in a sub-Saharan African country with higher spatial resolution providing new insights into bTB. Four hundred and ninety-nine samples were collected for culture from 201 and 179 cattle with and without bTB-like lesions respectively out of 2,346 cattle slaughtered at Bamenda, Ngaoundere, Garoua and Maroua abattoirs between 2012–2013. Two hundred and fifty-five M. bovis were isolated, identified and genotyped using deletion analysis, Hain® Genotype MTBC, spoligotyping and MIRU-VNTR. African 1 was the dominant M. bovis clonal complex, with 97 unique genotypes including 19 novel spoligotypes representing the highest M. bovis genetic diversity observed in Africa to date. SB0944 and SB0953 dominated (63%) the observed spoligotypes. A third of animals with multiple lesions had multiple strain infections. Higher diversity but little evidence of recent transmission of M. bovis was more common in Adamawa compared to the North-West Region. The Adamawa was characterised by a high frequency of singletons possibly due to constant additions from an active livestock movement network compared to the North-West Region where a local expansion was more evident. The latter combined with population-based inferences suggest an unstable and stable bTB-endemic status in the North-West and Adamawa Regions respectively.
format	article
author	N. F. Egbe A. Muwonge L. Ndip R. F. Kelly M. Sander V. Tanya V. Ngu Ngwa I. G. Handel A. Novak R. Ngandalo S. Mazeri K. L. Morgan A. Asuquo B. M. de C. Bronsvoort
author_facet	N. F. Egbe A. Muwonge L. Ndip R. F. Kelly M. Sander V. Tanya V. Ngu Ngwa I. G. Handel A. Novak R. Ngandalo S. Mazeri K. L. Morgan A. Asuquo B. M. de C. Bronsvoort
author_sort	N. F. Egbe
title	Molecular epidemiology of Mycobacterium bovis in Cameroon
title_short	Molecular epidemiology of Mycobacterium bovis in Cameroon
title_full	Molecular epidemiology of Mycobacterium bovis in Cameroon
title_fullStr	Molecular epidemiology of Mycobacterium bovis in Cameroon
title_full_unstemmed	Molecular epidemiology of Mycobacterium bovis in Cameroon
title_sort	molecular epidemiology of mycobacterium bovis in cameroon
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d62a1dd093904985bed3e2041c035f91
work_keys_str_mv	AT nfegbe molecularepidemiologyofmycobacteriumbovisincameroon AT amuwonge molecularepidemiologyofmycobacteriumbovisincameroon AT lndip molecularepidemiologyofmycobacteriumbovisincameroon AT rfkelly molecularepidemiologyofmycobacteriumbovisincameroon AT msander molecularepidemiologyofmycobacteriumbovisincameroon AT vtanya molecularepidemiologyofmycobacteriumbovisincameroon AT vngungwa molecularepidemiologyofmycobacteriumbovisincameroon AT ighandel molecularepidemiologyofmycobacteriumbovisincameroon AT anovak molecularepidemiologyofmycobacteriumbovisincameroon AT rngandalo molecularepidemiologyofmycobacteriumbovisincameroon AT smazeri molecularepidemiologyofmycobacteriumbovisincameroon AT klmorgan molecularepidemiologyofmycobacteriumbovisincameroon AT aasuquo molecularepidemiologyofmycobacteriumbovisincameroon AT bmdecbronsvoort molecularepidemiologyofmycobacteriumbovisincameroon
_version_	1718384991470092288

Molecular epidemiology of Mycobacterium bovis in Cameroon

Ejemplares similares