A proteomic-informed view of the changes induced by loss of cellular adherence: The example of mouse macrophages.
Except cells circulating in the bloodstream, most cells in vertebrates are adherent. Studying the repercussions of adherence per se in cell physiology is thus very difficult to carry out, although it plays an important role in cancer biology, e.g. in the metastasis process. In order to study how adh...
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oai:doaj.org-article:d63958df8ca14344aa756ed03b7a78392021-12-02T20:11:13ZA proteomic-informed view of the changes induced by loss of cellular adherence: The example of mouse macrophages.1932-620310.1371/journal.pone.0252450https://doaj.org/article/d63958df8ca14344aa756ed03b7a78392021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252450https://doaj.org/toc/1932-6203Except cells circulating in the bloodstream, most cells in vertebrates are adherent. Studying the repercussions of adherence per se in cell physiology is thus very difficult to carry out, although it plays an important role in cancer biology, e.g. in the metastasis process. In order to study how adherence impacts major cell functions, we used a murine macrophage cell line. Opposite to the monocyte/macrophage system, where adherence is associated with the acquisition of differentiated functions, these cells can be grown in both adherent or suspension conditions without altering their differentiated functions (phagocytosis and inflammation signaling). We used a proteomic approach to cover a large panel of proteins potentially modified by the adherence status. Targeted experiments were carried out to validate the proteomic results, e.g. on metabolic enzymes, mitochondrial and cytoskeletal proteins. The mitochondrial activity was increased in non-adherent cells compared with adherent cells, without differences in glucose consumption. Concerning the cytoskeleton, a rearrangement of the actin organization (filopodia vs sub-cortical network) and of the microtubule network were observed between adherent and non-adherent cells. Taken together, these data show the mechanisms at play for the modification of the cytoskeleton and also modifications of the metabolic activity between adherent and non-adherent cells.Sacnite Ramirez RiosAnaelle TorresHélène DiemerVéronique Collin-FaureSarah CianféraniLaurence LafanechèreThierry RabilloudPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0252450 (2021) |
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Medicine R Science Q Sacnite Ramirez Rios Anaelle Torres Hélène Diemer Véronique Collin-Faure Sarah Cianférani Laurence Lafanechère Thierry Rabilloud A proteomic-informed view of the changes induced by loss of cellular adherence: The example of mouse macrophages. |
| description |
Except cells circulating in the bloodstream, most cells in vertebrates are adherent. Studying the repercussions of adherence per se in cell physiology is thus very difficult to carry out, although it plays an important role in cancer biology, e.g. in the metastasis process. In order to study how adherence impacts major cell functions, we used a murine macrophage cell line. Opposite to the monocyte/macrophage system, where adherence is associated with the acquisition of differentiated functions, these cells can be grown in both adherent or suspension conditions without altering their differentiated functions (phagocytosis and inflammation signaling). We used a proteomic approach to cover a large panel of proteins potentially modified by the adherence status. Targeted experiments were carried out to validate the proteomic results, e.g. on metabolic enzymes, mitochondrial and cytoskeletal proteins. The mitochondrial activity was increased in non-adherent cells compared with adherent cells, without differences in glucose consumption. Concerning the cytoskeleton, a rearrangement of the actin organization (filopodia vs sub-cortical network) and of the microtubule network were observed between adherent and non-adherent cells. Taken together, these data show the mechanisms at play for the modification of the cytoskeleton and also modifications of the metabolic activity between adherent and non-adherent cells. |
| format |
article |
| author |
Sacnite Ramirez Rios Anaelle Torres Hélène Diemer Véronique Collin-Faure Sarah Cianférani Laurence Lafanechère Thierry Rabilloud |
| author_facet |
Sacnite Ramirez Rios Anaelle Torres Hélène Diemer Véronique Collin-Faure Sarah Cianférani Laurence Lafanechère Thierry Rabilloud |
| author_sort |
Sacnite Ramirez Rios |
| title |
A proteomic-informed view of the changes induced by loss of cellular adherence: The example of mouse macrophages. |
| title_short |
A proteomic-informed view of the changes induced by loss of cellular adherence: The example of mouse macrophages. |
| title_full |
A proteomic-informed view of the changes induced by loss of cellular adherence: The example of mouse macrophages. |
| title_fullStr |
A proteomic-informed view of the changes induced by loss of cellular adherence: The example of mouse macrophages. |
| title_full_unstemmed |
A proteomic-informed view of the changes induced by loss of cellular adherence: The example of mouse macrophages. |
| title_sort |
proteomic-informed view of the changes induced by loss of cellular adherence: the example of mouse macrophages. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2021 |
| url |
https://doaj.org/article/d63958df8ca14344aa756ed03b7a7839 |
| work_keys_str_mv |
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| _version_ |
1718374913284243456 |