A novel type of self-assembled nanoparticles as targeted gene carriers: an application for plasmid DNA and antimicroRNA oligonucleotide delivery

A novel type of self-assembled nanoparticles as targeted gene carriers: an application for plasmid DNA and antimicroRNA oligonucleotide delivery

Yanliang Zhu,1 Gaofeng Liang,2 Bo Sun,1 Tian Tian,3 Feihu Hu,1 Zhongdang Xiao11State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 2School of Medical Technology and Engineering, Henan University of Science and Technology, Luoya...

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Autores principales: Zhu YL, Liang GF, Sun B, Tian T, Hu FH, Xiao ZD
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2016
Materias:
gene delivery
hyaluronic acid
polyethyleneimine
anti-miR-221
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/d64106c9bcf24ac0bf48154dd492e7a0
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spelling oai:doaj.org-article:d64106c9bcf24ac0bf48154dd492e7a02021-12-02T07:13:44ZA novel type of self-assembled nanoparticles as targeted gene carriers: an application for plasmid DNA and antimicroRNA oligonucleotide delivery1178-2013https://doaj.org/article/d64106c9bcf24ac0bf48154dd492e7a02016-01-01T00:00:00Zhttps://www.dovepress.com/a-novel-type-of-self-assembled-nanoparticles-as-targeted-gene-carriers-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yanliang Zhu,1 Gaofeng Liang,2 Bo Sun,1 Tian Tian,3 Feihu Hu,1 Zhongdang Xiao11State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 2School of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, 3Department of Neurobiology, Nanjing Medical University, Nanjing, People’s Republic of ChinaAbstract: In this study, a new type of amphiphilic cetylated polyethyleneimine (PEI) was synthesized, and then polylactic-co-glycolic acid (PLGA)/cetylated PEI/hyaluronic acid nanoparticles (PCPH NPs) were developed by self-assembly as a novel type of gene-delivering vehicle. The PCPH NPs showed good DNA-condensation ability by forming polyplexes with small particle size and positive zeta potential. The transfection efficiency and cytotoxicity of PCPH NPs were evaluated as plasmid DNA vectors to transfect HepG2 in vitro. PCPH NPs exhibited much lower cytotoxicity and higher gene-transfection efficiency than PEI (25,000) and commercial transfection reagents. Furthermore, PCPH NPs were used as an anti-miR-221 vector for transfecting HepG2 cells, and anti-miR-221 was effectively transfected into cells and produced a greater inhibitory effect on cancer-cell growth by PCPH NPs. These results demonstrate that PCPH NPs can be a promising nonviral vector for gene-delivery systems.Keywords: gene delivery, hyaluronic acid, polyethyleneimine, anti-miR-221, PLGAZhu YLLiang GFSun BTian THu FHXiao ZDDove Medical Pressarticlegene deliveryhyaluronic acidpolyethyleneimineanti-miR-221Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 399-411 (2016)
institution DOAJ
collection DOAJ
language EN
topic gene delivery
hyaluronic acid
polyethyleneimine
anti-miR-221
Medicine (General)
R5-920
spellingShingle gene delivery
hyaluronic acid
polyethyleneimine
anti-miR-221
Medicine (General)
R5-920
Zhu YL
Liang GF
Sun B
Tian T
Hu FH
Xiao ZD
A novel type of self-assembled nanoparticles as targeted gene carriers: an application for plasmid DNA and antimicroRNA oligonucleotide delivery
description Yanliang Zhu,1 Gaofeng Liang,2 Bo Sun,1 Tian Tian,3 Feihu Hu,1 Zhongdang Xiao11State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 2School of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, 3Department of Neurobiology, Nanjing Medical University, Nanjing, People’s Republic of ChinaAbstract: In this study, a new type of amphiphilic cetylated polyethyleneimine (PEI) was synthesized, and then polylactic-co-glycolic acid (PLGA)/cetylated PEI/hyaluronic acid nanoparticles (PCPH NPs) were developed by self-assembly as a novel type of gene-delivering vehicle. The PCPH NPs showed good DNA-condensation ability by forming polyplexes with small particle size and positive zeta potential. The transfection efficiency and cytotoxicity of PCPH NPs were evaluated as plasmid DNA vectors to transfect HepG2 in vitro. PCPH NPs exhibited much lower cytotoxicity and higher gene-transfection efficiency than PEI (25,000) and commercial transfection reagents. Furthermore, PCPH NPs were used as an anti-miR-221 vector for transfecting HepG2 cells, and anti-miR-221 was effectively transfected into cells and produced a greater inhibitory effect on cancer-cell growth by PCPH NPs. These results demonstrate that PCPH NPs can be a promising nonviral vector for gene-delivery systems.Keywords: gene delivery, hyaluronic acid, polyethyleneimine, anti-miR-221, PLGA
format article
author Zhu YL
Liang GF
Sun B
Tian T
Hu FH
Xiao ZD
author_facet Zhu YL
Liang GF
Sun B
Tian T
Hu FH
Xiao ZD
author_sort Zhu YL
title A novel type of self-assembled nanoparticles as targeted gene carriers: an application for plasmid DNA and antimicroRNA oligonucleotide delivery
title_short A novel type of self-assembled nanoparticles as targeted gene carriers: an application for plasmid DNA and antimicroRNA oligonucleotide delivery
title_full A novel type of self-assembled nanoparticles as targeted gene carriers: an application for plasmid DNA and antimicroRNA oligonucleotide delivery
title_fullStr A novel type of self-assembled nanoparticles as targeted gene carriers: an application for plasmid DNA and antimicroRNA oligonucleotide delivery
title_full_unstemmed A novel type of self-assembled nanoparticles as targeted gene carriers: an application for plasmid DNA and antimicroRNA oligonucleotide delivery
title_sort novel type of self-assembled nanoparticles as targeted gene carriers: an application for plasmid dna and antimicrorna oligonucleotide delivery
publisher Dove Medical Press
publishDate 2016
url https://doaj.org/article/d64106c9bcf24ac0bf48154dd492e7a0
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