Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis

Cellular heterogeneity is of significance in cell-based assays for life science, biomedicine and clinical diagnostics. Electrical impedance sensing technology has become a powerful tool, allowing for rapid, non-invasive, and label-free acquisition of electrical parameters of single cells. These elec...

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Autores principales:	Zhao Zhang, Xiaowen Huang, Ke Liu, Tiancong Lan, Zixin Wang, Zhen Zhu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	electrical impedance spectroscopy impedance flow cytometry single cell analysis microfluidics Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/d643d5a2ea29456aa6d3b0eb382d5a23
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spelling	oai:doaj.org-article:d643d5a2ea29456aa6d3b0eb382d5a232021-11-25T16:55:54ZRecent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis10.3390/bios111104702079-6374https://doaj.org/article/d643d5a2ea29456aa6d3b0eb382d5a232021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/470https://doaj.org/toc/2079-6374Cellular heterogeneity is of significance in cell-based assays for life science, biomedicine and clinical diagnostics. Electrical impedance sensing technology has become a powerful tool, allowing for rapid, non-invasive, and label-free acquisition of electrical parameters of single cells. These electrical parameters, i.e., equivalent cell resistance, membrane capacitance and cytoplasm conductivity, are closely related to cellular biophysical properties and dynamic activities, such as size, morphology, membrane intactness, growth state, and proliferation. This review summarizes basic principles, analytical models and design concepts of single-cell impedance sensing devices, including impedance flow cytometry (IFC) to detect flow-through single cells and electrical impedance spectroscopy (EIS) to monitor immobilized single cells. Then, recent advances of both electrical impedance sensing systems applied in cell recognition, cell counting, viability detection, phenotypic assay, cell screening, and other cell detection are presented. Finally, prospects of impedance sensing technology in single-cell analysis are discussed.Zhao ZhangXiaowen HuangKe LiuTiancong LanZixin WangZhen ZhuMDPI AGarticleelectrical impedance spectroscopyimpedance flow cytometrysingle cell analysismicrofluidicsBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 470, p 470 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	electrical impedance spectroscopy impedance flow cytometry single cell analysis microfluidics Biotechnology TP248.13-248.65
spellingShingle	electrical impedance spectroscopy impedance flow cytometry single cell analysis microfluidics Biotechnology TP248.13-248.65 Zhao Zhang Xiaowen Huang Ke Liu Tiancong Lan Zixin Wang Zhen Zhu Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis
description	Cellular heterogeneity is of significance in cell-based assays for life science, biomedicine and clinical diagnostics. Electrical impedance sensing technology has become a powerful tool, allowing for rapid, non-invasive, and label-free acquisition of electrical parameters of single cells. These electrical parameters, i.e., equivalent cell resistance, membrane capacitance and cytoplasm conductivity, are closely related to cellular biophysical properties and dynamic activities, such as size, morphology, membrane intactness, growth state, and proliferation. This review summarizes basic principles, analytical models and design concepts of single-cell impedance sensing devices, including impedance flow cytometry (IFC) to detect flow-through single cells and electrical impedance spectroscopy (EIS) to monitor immobilized single cells. Then, recent advances of both electrical impedance sensing systems applied in cell recognition, cell counting, viability detection, phenotypic assay, cell screening, and other cell detection are presented. Finally, prospects of impedance sensing technology in single-cell analysis are discussed.
format	article
author	Zhao Zhang Xiaowen Huang Ke Liu Tiancong Lan Zixin Wang Zhen Zhu
author_facet	Zhao Zhang Xiaowen Huang Ke Liu Tiancong Lan Zixin Wang Zhen Zhu
author_sort	Zhao Zhang
title	Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis
title_short	Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis
title_full	Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis
title_fullStr	Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis
title_full_unstemmed	Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis
title_sort	recent advances in electrical impedance sensing technology for single-cell analysis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d643d5a2ea29456aa6d3b0eb382d5a23
work_keys_str_mv	AT zhaozhang recentadvancesinelectricalimpedancesensingtechnologyforsinglecellanalysis AT xiaowenhuang recentadvancesinelectricalimpedancesensingtechnologyforsinglecellanalysis AT keliu recentadvancesinelectricalimpedancesensingtechnologyforsinglecellanalysis AT tianconglan recentadvancesinelectricalimpedancesensingtechnologyforsinglecellanalysis AT zixinwang recentadvancesinelectricalimpedancesensingtechnologyforsinglecellanalysis AT zhenzhu recentadvancesinelectricalimpedancesensingtechnologyforsinglecellanalysis
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Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis

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