Improving T-cell assays for the diagnosis of latent TB infection: potential of a diagnostic test based on IP-10.
<h4>Background</h4>There is a need for simple tools such as the M.tuberculosis specific IFN-gamma release assays (IGRA) to improve diagnosis of M.tuberculosis-infection in children. The aim of the study was to evaluate the performance of an IP-10 and IL-2 based tests for the diagnosis of...
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2008
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oai:doaj.org-article:d64c3b0b26dc406b85bc2a53d603073f2021-11-25T06:11:19ZImproving T-cell assays for the diagnosis of latent TB infection: potential of a diagnostic test based on IP-10.1932-620310.1371/journal.pone.0002858https://doaj.org/article/d64c3b0b26dc406b85bc2a53d603073f2008-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18682747/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>There is a need for simple tools such as the M.tuberculosis specific IFN-gamma release assays (IGRA) to improve diagnosis of M.tuberculosis-infection in children. The aim of the study was to evaluate the performance of an IP-10 and IL-2 based tests for the diagnosis of M.tuberculosis-infection in recently exposed children from Nigeria.<h4>Methodology and principal findings</h4>Samples were obtained from 59 children at high risk of infection with M.tuberculosis (contacts of adults with smear and culture-positive tuberculosis) and 61 at low risk (contacts of smear-negative/culture-positive tuberculosis or community controls). IP-10 and IL-2 was measured in plasma after stimulation of whole-blood with M.tuberculosis specific antigens and mitogen. Previously developed criteria for positive IP-10 and IL-2 tests were used and the diagnostic performances of the IP-10 and IL-2 tests were compared with the Quantiferon In-Tube (QFT-IT) and the Tuberculin Skin Tests (TST). In response to M.tuberculosis specific antigens, the high-risk children expressed significantly higher levels of IP-10 (1358 pg/ml[IQR 278-2535 pg/ml]) and IL-2 (164 pg/ml[11-590 pg/ml]) than low risk groups 149 pg/ml(25-497 pg/ml), and 0 pg/ml(0-3 pg/ml), respectively. There was excellent agreement (>89%,k>0.80) between IP-10, IL-2 tests and QFT-IT, better than with TST (>74%,k>0.49). The IP-10 and IL-2 responses were strongly associated with M.tuberculosis exposure and with grade of infectiousness of the index cases (p<0.0001). IP-10, IL-2, and TST but not QFT-IT was associated with age of the child in the low risk groups (p<0.02).<h4>Conclusions/significance</h4>IP-10 is expressed in high levels and results of the IP-10 test were comparable to the QFT-IT. IL-2 was released in low amounts in response to the antigens and not in response to the mitogen therefore IL-2 seems a less useful marker. We have demonstrated that IP-10 and possibly IL-2 could be alternative or adjunct markers to IFN-gamma in the diagnosis infection with M.tuberculosis.Morten RuhwaldJanne PetersenKristian KofoedHiroshi NakaokaLuis Eduardo CuevasLovett LawsonStephen Bertil SquireJesper Eugen-OlsenPernille RavnPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 8, p e2858 (2008) |
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Medicine R Science Q Morten Ruhwald Janne Petersen Kristian Kofoed Hiroshi Nakaoka Luis Eduardo Cuevas Lovett Lawson Stephen Bertil Squire Jesper Eugen-Olsen Pernille Ravn Improving T-cell assays for the diagnosis of latent TB infection: potential of a diagnostic test based on IP-10. |
| description |
<h4>Background</h4>There is a need for simple tools such as the M.tuberculosis specific IFN-gamma release assays (IGRA) to improve diagnosis of M.tuberculosis-infection in children. The aim of the study was to evaluate the performance of an IP-10 and IL-2 based tests for the diagnosis of M.tuberculosis-infection in recently exposed children from Nigeria.<h4>Methodology and principal findings</h4>Samples were obtained from 59 children at high risk of infection with M.tuberculosis (contacts of adults with smear and culture-positive tuberculosis) and 61 at low risk (contacts of smear-negative/culture-positive tuberculosis or community controls). IP-10 and IL-2 was measured in plasma after stimulation of whole-blood with M.tuberculosis specific antigens and mitogen. Previously developed criteria for positive IP-10 and IL-2 tests were used and the diagnostic performances of the IP-10 and IL-2 tests were compared with the Quantiferon In-Tube (QFT-IT) and the Tuberculin Skin Tests (TST). In response to M.tuberculosis specific antigens, the high-risk children expressed significantly higher levels of IP-10 (1358 pg/ml[IQR 278-2535 pg/ml]) and IL-2 (164 pg/ml[11-590 pg/ml]) than low risk groups 149 pg/ml(25-497 pg/ml), and 0 pg/ml(0-3 pg/ml), respectively. There was excellent agreement (>89%,k>0.80) between IP-10, IL-2 tests and QFT-IT, better than with TST (>74%,k>0.49). The IP-10 and IL-2 responses were strongly associated with M.tuberculosis exposure and with grade of infectiousness of the index cases (p<0.0001). IP-10, IL-2, and TST but not QFT-IT was associated with age of the child in the low risk groups (p<0.02).<h4>Conclusions/significance</h4>IP-10 is expressed in high levels and results of the IP-10 test were comparable to the QFT-IT. IL-2 was released in low amounts in response to the antigens and not in response to the mitogen therefore IL-2 seems a less useful marker. We have demonstrated that IP-10 and possibly IL-2 could be alternative or adjunct markers to IFN-gamma in the diagnosis infection with M.tuberculosis. |
| format |
article |
| author |
Morten Ruhwald Janne Petersen Kristian Kofoed Hiroshi Nakaoka Luis Eduardo Cuevas Lovett Lawson Stephen Bertil Squire Jesper Eugen-Olsen Pernille Ravn |
| author_facet |
Morten Ruhwald Janne Petersen Kristian Kofoed Hiroshi Nakaoka Luis Eduardo Cuevas Lovett Lawson Stephen Bertil Squire Jesper Eugen-Olsen Pernille Ravn |
| author_sort |
Morten Ruhwald |
| title |
Improving T-cell assays for the diagnosis of latent TB infection: potential of a diagnostic test based on IP-10. |
| title_short |
Improving T-cell assays for the diagnosis of latent TB infection: potential of a diagnostic test based on IP-10. |
| title_full |
Improving T-cell assays for the diagnosis of latent TB infection: potential of a diagnostic test based on IP-10. |
| title_fullStr |
Improving T-cell assays for the diagnosis of latent TB infection: potential of a diagnostic test based on IP-10. |
| title_full_unstemmed |
Improving T-cell assays for the diagnosis of latent TB infection: potential of a diagnostic test based on IP-10. |
| title_sort |
improving t-cell assays for the diagnosis of latent tb infection: potential of a diagnostic test based on ip-10. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2008 |
| url |
https://doaj.org/article/d64c3b0b26dc406b85bc2a53d603073f |
| work_keys_str_mv |
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