The Effect of Silk Nanofibrous Scaffold and Co-Culture with Sertoli Cells on Spermatogonial Stem Cell Proliferation

BACKGROUND AND OBJECTIVE: In vitro proliferation and maintenance of spermatogonial stem cells is one of the treatment options for infertile men. Creating a suitable microenvironment similar to the natural conditions of reproduction of these cells leads to the improvement of culture and spermatogenes...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Z Narimanpour, M Nazm Bojnordi, H Ghasemi
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2021
Materias:	scaffold silk sertoli proliferation spermatogonia. Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d64f7860b0ad4bd5bcd321dbe0f270a5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d64f7860b0ad4bd5bcd321dbe0f270a5
record_format	dspace
spelling	oai:doaj.org-article:d64f7860b0ad4bd5bcd321dbe0f270a52021-11-29T07:40:49ZThe Effect of Silk Nanofibrous Scaffold and Co-Culture with Sertoli Cells on Spermatogonial Stem Cell Proliferation1561-41072251-7170https://doaj.org/article/d64f7860b0ad4bd5bcd321dbe0f270a52021-03-01T00:00:00Zhttp://jbums.org/article-1-9606-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: In vitro proliferation and maintenance of spermatogonial stem cells is one of the treatment options for infertile men. Creating a suitable microenvironment similar to the natural conditions of reproduction of these cells leads to the improvement of culture and spermatogenesis. Therefore, the aim of the present study was to use a 3D silk nanofibrous electrospun scaffold and co-culture with Sertoli cells in order to increase the proliferation of spermatogonial stem cells. METHODS: In this experimental study, spermatogonial stem cells were isolated from the testes of 50 2-4-day-old mice (5 samples each time) using two-step mechanical and enzymatic digestion within two weeks. After placing 2×104 cells on the surface of the silk scaffold, they were exposed to culture medium. The viability of these cells was assessed using MTT assay and their binding was evaluated by SEM microscopy. The studied variables, including Stra8, DAZL and Piwill2 specific genes were analyzed by real time PCR and immunocytochemistry. FINDINGS: The viability of cultured cells in the presence of scaffold and Sertoli (91±6), (90±2) was higher than the control group (85±2), (83±8). Real time PCR results confirmed a significant increase in the expression of specific markers of spermatogonial stem cells such as Stra8, Piwill2 and DAZL in cells cultured respectively on scaffold (1.47±6) (1.28±5) (1.43±2) compared to the 2D culture group (1.17±5) (1.05±3) (1.13±7) (p<0.05). CONCLUSION: The results showed that silk scaffold in the presence of Sertoli cell co-culture can increase in vitro proliferation of spermatogonial stem cells and can have a positive effect on the viability and proliferation of these cells.Z NarimanpourM Nazm BojnordiH GhasemiBabol University of Medical Sciencesarticlescaffoldsilksertoliproliferationspermatogonia.MedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 23, Iss 1, Pp 208-214 (2021)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	scaffold silk sertoli proliferation spermatogonia. Medicine R Medicine (General) R5-920
spellingShingle	scaffold silk sertoli proliferation spermatogonia. Medicine R Medicine (General) R5-920 Z Narimanpour M Nazm Bojnordi H Ghasemi The Effect of Silk Nanofibrous Scaffold and Co-Culture with Sertoli Cells on Spermatogonial Stem Cell Proliferation
description	BACKGROUND AND OBJECTIVE: In vitro proliferation and maintenance of spermatogonial stem cells is one of the treatment options for infertile men. Creating a suitable microenvironment similar to the natural conditions of reproduction of these cells leads to the improvement of culture and spermatogenesis. Therefore, the aim of the present study was to use a 3D silk nanofibrous electrospun scaffold and co-culture with Sertoli cells in order to increase the proliferation of spermatogonial stem cells. METHODS: In this experimental study, spermatogonial stem cells were isolated from the testes of 50 2-4-day-old mice (5 samples each time) using two-step mechanical and enzymatic digestion within two weeks. After placing 2×104 cells on the surface of the silk scaffold, they were exposed to culture medium. The viability of these cells was assessed using MTT assay and their binding was evaluated by SEM microscopy. The studied variables, including Stra8, DAZL and Piwill2 specific genes were analyzed by real time PCR and immunocytochemistry. FINDINGS: The viability of cultured cells in the presence of scaffold and Sertoli (91±6), (90±2) was higher than the control group (85±2), (83±8). Real time PCR results confirmed a significant increase in the expression of specific markers of spermatogonial stem cells such as Stra8, Piwill2 and DAZL in cells cultured respectively on scaffold (1.47±6) (1.28±5) (1.43±2) compared to the 2D culture group (1.17±5) (1.05±3) (1.13±7) (p<0.05). CONCLUSION: The results showed that silk scaffold in the presence of Sertoli cell co-culture can increase in vitro proliferation of spermatogonial stem cells and can have a positive effect on the viability and proliferation of these cells.
format	article
author	Z Narimanpour M Nazm Bojnordi H Ghasemi
author_facet	Z Narimanpour M Nazm Bojnordi H Ghasemi
author_sort	Z Narimanpour
title	The Effect of Silk Nanofibrous Scaffold and Co-Culture with Sertoli Cells on Spermatogonial Stem Cell Proliferation
title_short	The Effect of Silk Nanofibrous Scaffold and Co-Culture with Sertoli Cells on Spermatogonial Stem Cell Proliferation
title_full	The Effect of Silk Nanofibrous Scaffold and Co-Culture with Sertoli Cells on Spermatogonial Stem Cell Proliferation
title_fullStr	The Effect of Silk Nanofibrous Scaffold and Co-Culture with Sertoli Cells on Spermatogonial Stem Cell Proliferation
title_full_unstemmed	The Effect of Silk Nanofibrous Scaffold and Co-Culture with Sertoli Cells on Spermatogonial Stem Cell Proliferation
title_sort	effect of silk nanofibrous scaffold and co-culture with sertoli cells on spermatogonial stem cell proliferation
publisher	Babol University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/d64f7860b0ad4bd5bcd321dbe0f270a5
work_keys_str_mv	AT znarimanpour theeffectofsilknanofibrousscaffoldandcoculturewithsertolicellsonspermatogonialstemcellproliferation AT mnazmbojnordi theeffectofsilknanofibrousscaffoldandcoculturewithsertolicellsonspermatogonialstemcellproliferation AT hghasemi theeffectofsilknanofibrousscaffoldandcoculturewithsertolicellsonspermatogonialstemcellproliferation AT znarimanpour effectofsilknanofibrousscaffoldandcoculturewithsertolicellsonspermatogonialstemcellproliferation AT mnazmbojnordi effectofsilknanofibrousscaffoldandcoculturewithsertolicellsonspermatogonialstemcellproliferation AT hghasemi effectofsilknanofibrousscaffoldandcoculturewithsertolicellsonspermatogonialstemcellproliferation
_version_	1718407554491482112

The Effect of Silk Nanofibrous Scaffold and Co-Culture with Sertoli Cells on Spermatogonial Stem Cell Proliferation

Ejemplares similares