Quercetin is more effective than cromolyn in blocking human mast cell cytokine release and inhibits contact dermatitis and photosensitivity in humans.

Mast cells are immune cells critical in the pathogenesis of allergic, but also inflammatory and autoimmune diseases through release of many pro-inflammatory cytokines such as IL-8 and TNF. Contact dermatitis and photosensitivity are skin conditions that involve non-immune triggers such as substance...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zuyi Weng, Bodi Zhang, Shahrzad Asadi, Nikolaos Sismanopoulos, Alan Butcher, Xueyan Fu, Alexandra Katsarou-Katsari, Christina Antoniou, Theoharis C Theoharides
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d64fcb783d6c43c7ac07226ec61045ce
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d64fcb783d6c43c7ac07226ec61045ce
record_format	dspace
spelling	oai:doaj.org-article:d64fcb783d6c43c7ac07226ec61045ce2021-11-18T07:24:04ZQuercetin is more effective than cromolyn in blocking human mast cell cytokine release and inhibits contact dermatitis and photosensitivity in humans.1932-620310.1371/journal.pone.0033805https://doaj.org/article/d64fcb783d6c43c7ac07226ec61045ce2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22470478/?tool=EBIhttps://doaj.org/toc/1932-6203Mast cells are immune cells critical in the pathogenesis of allergic, but also inflammatory and autoimmune diseases through release of many pro-inflammatory cytokines such as IL-8 and TNF. Contact dermatitis and photosensitivity are skin conditions that involve non-immune triggers such as substance P (SP), and do not respond to conventional treatment. Inhibition of mast cell cytokine release could be effective therapy for such diseases. Unfortunately, disodium cromoglycate (cromolyn), the only compound marketed as a mast cell "stabilizer", is not particularly effective in blocking human mast cells. Instead, flavonoids are potent anti-oxidant and anti-inflammatory compounds with mast cell inhibitory actions. Here, we first compared the flavonoid quercetin (Que) and cromolyn on cultured human mast cells. Que and cromolyn (100 µM) can effectively inhibit secretion of histamine and PGD(2). Que and cromolyn also inhibit histamine, leukotrienes and PGD(2) from primary human cord blood-derived cultured mast cells (hCBMCs) stimulated by IgE/Anti-IgE. However, Que is more effective than cromolyn in inhibiting IL-8 and TNF release from LAD2 mast cells stimulated by SP. Moreover, Que reduces IL-6 release from hCBMCs in a dose-dependent manner. Que inhibits cytosolic calcium level increase and NF-kappa B activation. Interestingly, Que is effective prophylactically, while cromolyn must be added together with the trigger or it rapidly loses its effect. In two pilot, open-label, clinical trials, Que significantly decreased contact dermatitis and photosensitivity, skin conditions that do not respond to conventional treatment. In summary, Que is a promising candidate as an effective mast cell inhibitor for allergic and inflammatory diseases, especially in formulations that permit more sufficient oral absorption.Zuyi WengBodi ZhangShahrzad AsadiNikolaos SismanopoulosAlan ButcherXueyan FuAlexandra Katsarou-KatsariChristina AntoniouTheoharis C TheoharidesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e33805 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zuyi Weng Bodi Zhang Shahrzad Asadi Nikolaos Sismanopoulos Alan Butcher Xueyan Fu Alexandra Katsarou-Katsari Christina Antoniou Theoharis C Theoharides Quercetin is more effective than cromolyn in blocking human mast cell cytokine release and inhibits contact dermatitis and photosensitivity in humans.
description	Mast cells are immune cells critical in the pathogenesis of allergic, but also inflammatory and autoimmune diseases through release of many pro-inflammatory cytokines such as IL-8 and TNF. Contact dermatitis and photosensitivity are skin conditions that involve non-immune triggers such as substance P (SP), and do not respond to conventional treatment. Inhibition of mast cell cytokine release could be effective therapy for such diseases. Unfortunately, disodium cromoglycate (cromolyn), the only compound marketed as a mast cell "stabilizer", is not particularly effective in blocking human mast cells. Instead, flavonoids are potent anti-oxidant and anti-inflammatory compounds with mast cell inhibitory actions. Here, we first compared the flavonoid quercetin (Que) and cromolyn on cultured human mast cells. Que and cromolyn (100 µM) can effectively inhibit secretion of histamine and PGD(2). Que and cromolyn also inhibit histamine, leukotrienes and PGD(2) from primary human cord blood-derived cultured mast cells (hCBMCs) stimulated by IgE/Anti-IgE. However, Que is more effective than cromolyn in inhibiting IL-8 and TNF release from LAD2 mast cells stimulated by SP. Moreover, Que reduces IL-6 release from hCBMCs in a dose-dependent manner. Que inhibits cytosolic calcium level increase and NF-kappa B activation. Interestingly, Que is effective prophylactically, while cromolyn must be added together with the trigger or it rapidly loses its effect. In two pilot, open-label, clinical trials, Que significantly decreased contact dermatitis and photosensitivity, skin conditions that do not respond to conventional treatment. In summary, Que is a promising candidate as an effective mast cell inhibitor for allergic and inflammatory diseases, especially in formulations that permit more sufficient oral absorption.
format	article
author	Zuyi Weng Bodi Zhang Shahrzad Asadi Nikolaos Sismanopoulos Alan Butcher Xueyan Fu Alexandra Katsarou-Katsari Christina Antoniou Theoharis C Theoharides
author_facet	Zuyi Weng Bodi Zhang Shahrzad Asadi Nikolaos Sismanopoulos Alan Butcher Xueyan Fu Alexandra Katsarou-Katsari Christina Antoniou Theoharis C Theoharides
author_sort	Zuyi Weng
title	Quercetin is more effective than cromolyn in blocking human mast cell cytokine release and inhibits contact dermatitis and photosensitivity in humans.
title_short	Quercetin is more effective than cromolyn in blocking human mast cell cytokine release and inhibits contact dermatitis and photosensitivity in humans.
title_full	Quercetin is more effective than cromolyn in blocking human mast cell cytokine release and inhibits contact dermatitis and photosensitivity in humans.
title_fullStr	Quercetin is more effective than cromolyn in blocking human mast cell cytokine release and inhibits contact dermatitis and photosensitivity in humans.
title_full_unstemmed	Quercetin is more effective than cromolyn in blocking human mast cell cytokine release and inhibits contact dermatitis and photosensitivity in humans.
title_sort	quercetin is more effective than cromolyn in blocking human mast cell cytokine release and inhibits contact dermatitis and photosensitivity in humans.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d64fcb783d6c43c7ac07226ec61045ce
work_keys_str_mv	AT zuyiweng quercetinismoreeffectivethancromolyninblockinghumanmastcellcytokinereleaseandinhibitscontactdermatitisandphotosensitivityinhumans AT bodizhang quercetinismoreeffectivethancromolyninblockinghumanmastcellcytokinereleaseandinhibitscontactdermatitisandphotosensitivityinhumans AT shahrzadasadi quercetinismoreeffectivethancromolyninblockinghumanmastcellcytokinereleaseandinhibitscontactdermatitisandphotosensitivityinhumans AT nikolaossismanopoulos quercetinismoreeffectivethancromolyninblockinghumanmastcellcytokinereleaseandinhibitscontactdermatitisandphotosensitivityinhumans AT alanbutcher quercetinismoreeffectivethancromolyninblockinghumanmastcellcytokinereleaseandinhibitscontactdermatitisandphotosensitivityinhumans AT xueyanfu quercetinismoreeffectivethancromolyninblockinghumanmastcellcytokinereleaseandinhibitscontactdermatitisandphotosensitivityinhumans AT alexandrakatsaroukatsari quercetinismoreeffectivethancromolyninblockinghumanmastcellcytokinereleaseandinhibitscontactdermatitisandphotosensitivityinhumans AT christinaantoniou quercetinismoreeffectivethancromolyninblockinghumanmastcellcytokinereleaseandinhibitscontactdermatitisandphotosensitivityinhumans AT theoharisctheoharides quercetinismoreeffectivethancromolyninblockinghumanmastcellcytokinereleaseandinhibitscontactdermatitisandphotosensitivityinhumans
_version_	1718423565248757760

Quercetin is more effective than cromolyn in blocking human mast cell cytokine release and inhibits contact dermatitis and photosensitivity in humans.

Ejemplares similares