Genome-wide characterization of peptidyl-prolyl cis–trans isomerases in Penicillium and their regulation by salt stress in a halotolerant P. oxalicum

Abstract Peptidyl-prolyl cis–trans isomerases (PPIases) are the only class of enzymes capable of cis–trans isomerization of the prolyl peptide bond. The PPIases, comprising of different families viz., cyclophilins, FK506-binding proteins (FKBPs), parvulins and protein phosphatase 2A phosphatase acti...

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Autores principales:	Mangaljeet Singh, Kirandeep Kaur, Avinash Sharma, Rajvir Kaur, Dimple Joshi, Megha Chatterjee, Iman Dandapath, Amarjeet Kaur, Harpreet Singh, Prabhjeet Singh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d651970954f3492581fd42fc887f6f39
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spelling	oai:doaj.org-article:d651970954f3492581fd42fc887f6f392021-12-02T17:34:35ZGenome-wide characterization of peptidyl-prolyl cis–trans isomerases in Penicillium and their regulation by salt stress in a halotolerant P. oxalicum10.1038/s41598-021-91602-82045-2322https://doaj.org/article/d651970954f3492581fd42fc887f6f392021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91602-8https://doaj.org/toc/2045-2322Abstract Peptidyl-prolyl cis–trans isomerases (PPIases) are the only class of enzymes capable of cis–trans isomerization of the prolyl peptide bond. The PPIases, comprising of different families viz., cyclophilins, FK506-binding proteins (FKBPs), parvulins and protein phosphatase 2A phosphatase activators (PTPAs), play essential roles in different cellular processes. Though PPIase gene families have been characterized in different organisms, information regarding these proteins is lacking in Penicillium species, which are commercially an important fungi group. In this study, we carried out genome-wide analysis of PPIases in different Penicillium spp. and investigated their regulation by salt stress in a halotolerant strain of Penicillium oxalicum. These analyses revealed that the number of genes encoding cyclophilins, FKBPs, parvulins and PTPAs in Penicillium spp. varies between 7–11, 2–5, 1–2, and 1–2, respectively. The halotolerant P. oxalicum depicted significant enhancement in the mycelial PPIase activity in the presence of 15% NaCl, thus, highlighting the role of these enzymes in salt stress adaptation. The stress-induced increase in PPIase activity at 4 and 10 DAI in P. oxalicum was associated with higher expression of PoxCYP18. Characterization of PPIases in Penicillium spp. will provide an important database for understanding their cellular functions and might facilitate their applications in industrial processes through biotechnological interventions.Mangaljeet SinghKirandeep KaurAvinash SharmaRajvir KaurDimple JoshiMegha ChatterjeeIman DandapathAmarjeet KaurHarpreet SinghPrabhjeet SinghNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mangaljeet Singh Kirandeep Kaur Avinash Sharma Rajvir Kaur Dimple Joshi Megha Chatterjee Iman Dandapath Amarjeet Kaur Harpreet Singh Prabhjeet Singh Genome-wide characterization of peptidyl-prolyl cis–trans isomerases in Penicillium and their regulation by salt stress in a halotolerant P. oxalicum
description	Abstract Peptidyl-prolyl cis–trans isomerases (PPIases) are the only class of enzymes capable of cis–trans isomerization of the prolyl peptide bond. The PPIases, comprising of different families viz., cyclophilins, FK506-binding proteins (FKBPs), parvulins and protein phosphatase 2A phosphatase activators (PTPAs), play essential roles in different cellular processes. Though PPIase gene families have been characterized in different organisms, information regarding these proteins is lacking in Penicillium species, which are commercially an important fungi group. In this study, we carried out genome-wide analysis of PPIases in different Penicillium spp. and investigated their regulation by salt stress in a halotolerant strain of Penicillium oxalicum. These analyses revealed that the number of genes encoding cyclophilins, FKBPs, parvulins and PTPAs in Penicillium spp. varies between 7–11, 2–5, 1–2, and 1–2, respectively. The halotolerant P. oxalicum depicted significant enhancement in the mycelial PPIase activity in the presence of 15% NaCl, thus, highlighting the role of these enzymes in salt stress adaptation. The stress-induced increase in PPIase activity at 4 and 10 DAI in P. oxalicum was associated with higher expression of PoxCYP18. Characterization of PPIases in Penicillium spp. will provide an important database for understanding their cellular functions and might facilitate their applications in industrial processes through biotechnological interventions.
format	article
author	Mangaljeet Singh Kirandeep Kaur Avinash Sharma Rajvir Kaur Dimple Joshi Megha Chatterjee Iman Dandapath Amarjeet Kaur Harpreet Singh Prabhjeet Singh
author_facet	Mangaljeet Singh Kirandeep Kaur Avinash Sharma Rajvir Kaur Dimple Joshi Megha Chatterjee Iman Dandapath Amarjeet Kaur Harpreet Singh Prabhjeet Singh
author_sort	Mangaljeet Singh
title	Genome-wide characterization of peptidyl-prolyl cis–trans isomerases in Penicillium and their regulation by salt stress in a halotolerant P. oxalicum
title_short	Genome-wide characterization of peptidyl-prolyl cis–trans isomerases in Penicillium and their regulation by salt stress in a halotolerant P. oxalicum
title_full	Genome-wide characterization of peptidyl-prolyl cis–trans isomerases in Penicillium and their regulation by salt stress in a halotolerant P. oxalicum
title_fullStr	Genome-wide characterization of peptidyl-prolyl cis–trans isomerases in Penicillium and their regulation by salt stress in a halotolerant P. oxalicum
title_full_unstemmed	Genome-wide characterization of peptidyl-prolyl cis–trans isomerases in Penicillium and their regulation by salt stress in a halotolerant P. oxalicum
title_sort	genome-wide characterization of peptidyl-prolyl cis–trans isomerases in penicillium and their regulation by salt stress in a halotolerant p. oxalicum
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d651970954f3492581fd42fc887f6f39
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Genome-wide characterization of peptidyl-prolyl cis–trans isomerases in Penicillium and their regulation by salt stress in a halotolerant P. oxalicum

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