Enhancement of anticancer efficacy using modified lipophilic nanoparticle drug encapsulation

Puiyan Lee1, Ruizhong Zhang1, Vincent Li1, Xuelai Liu1, Raymond WY Sun2, Chi-Ming Che2, Kenneth KY Wong11Department of Surgery, Li Ka Shing Faculty of Medicine, 2Department of Chemistry and Open Laboratory of Chemical Biology of the Institute of Molecular Technology for Drug Discovery and Synthesis,...

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Autores principales:	Lee P, Zhang R, Li V, Liu X, Sun RWY, Che CM, Wong KKY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d657e2769eae463db493c73c63cc4a9d
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spelling	oai:doaj.org-article:d657e2769eae463db493c73c63cc4a9d2021-12-02T07:14:18ZEnhancement of anticancer efficacy using modified lipophilic nanoparticle drug encapsulation1176-91141178-2013https://doaj.org/article/d657e2769eae463db493c73c63cc4a9d2012-02-01T00:00:00Zhttp://www.dovepress.com/enhancement-of-anticancer-efficacy-using-modified-lipophilic-nanoparti-a9241https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Puiyan Lee1, Ruizhong Zhang1, Vincent Li1, Xuelai Liu1, Raymond WY Sun2, Chi-Ming Che2, Kenneth KY Wong11Department of Surgery, Li Ka Shing Faculty of Medicine, 2Department of Chemistry and Open Laboratory of Chemical Biology of the Institute of Molecular Technology for Drug Discovery and Synthesis, The University of Hong Kong, Hong KongBackground: Development of anticancer drugs is challenging. Indeed, much research effort has been spent in the development of new drugs to improve clinical outcomes with minimal toxicity. We have previously reported that a formulation of lipid gold porphyrin nanoparticles reduced systemic drug toxicity when compared with free gold porphyrin. In this study, we investigated the delivery and treatment efficiency of PEG surface-modified lipid nanoparticles as a carrier platform.Methods: We encapsulated antitumor drugs into PEG-modified lipid nanoparticles and these were characterized by size, zeta potential, and encapsulation efficiency. The delivery efficiency into tumor tissue was evaluated using a biodistribution study. To evaluate antitumor efficacy, gold porphyrin or camptothecin (a DNA topoisomerase I inhibitor) were encapsulated and compared using an in vivo neuroblastoma (N2A) model.Results: We showed that drug encapsulation into PEG-modified lipid nanoparticles enhanced the preferential uptake in tumor tissue. Furthermore, higher tumor killing efficiency was observed in response to treatment with PEG-modified lipid nanoparticles encapsulating gold porphyrin or camptothecin when compared with free gold porphyrin or free camptothecin. The in vivo antitumor effect was further confirmed by study of tumor inhibition and positive apoptosis activity. Surface modification of lipophilic nanoparticles with PEG increased the efficiency of drug delivery into tumor tissue and subsequently more effective antitumor activity.Conclusion: This specific design of a chemotherapeutic agent using nanotechnology is important in the development of a safe and effective drug in cancer therapy.Keywords: lipid nanoparticles, gold porphyrin, cancer, neuroblastoma, camptothecinLee PZhang RLi VLiu XSun RWYChe CMWong KKYDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 731-737 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Lee P Zhang R Li V Liu X Sun RWY Che CM Wong KKY Enhancement of anticancer efficacy using modified lipophilic nanoparticle drug encapsulation
description	Puiyan Lee1, Ruizhong Zhang1, Vincent Li1, Xuelai Liu1, Raymond WY Sun2, Chi-Ming Che2, Kenneth KY Wong11Department of Surgery, Li Ka Shing Faculty of Medicine, 2Department of Chemistry and Open Laboratory of Chemical Biology of the Institute of Molecular Technology for Drug Discovery and Synthesis, The University of Hong Kong, Hong KongBackground: Development of anticancer drugs is challenging. Indeed, much research effort has been spent in the development of new drugs to improve clinical outcomes with minimal toxicity. We have previously reported that a formulation of lipid gold porphyrin nanoparticles reduced systemic drug toxicity when compared with free gold porphyrin. In this study, we investigated the delivery and treatment efficiency of PEG surface-modified lipid nanoparticles as a carrier platform.Methods: We encapsulated antitumor drugs into PEG-modified lipid nanoparticles and these were characterized by size, zeta potential, and encapsulation efficiency. The delivery efficiency into tumor tissue was evaluated using a biodistribution study. To evaluate antitumor efficacy, gold porphyrin or camptothecin (a DNA topoisomerase I inhibitor) were encapsulated and compared using an in vivo neuroblastoma (N2A) model.Results: We showed that drug encapsulation into PEG-modified lipid nanoparticles enhanced the preferential uptake in tumor tissue. Furthermore, higher tumor killing efficiency was observed in response to treatment with PEG-modified lipid nanoparticles encapsulating gold porphyrin or camptothecin when compared with free gold porphyrin or free camptothecin. The in vivo antitumor effect was further confirmed by study of tumor inhibition and positive apoptosis activity. Surface modification of lipophilic nanoparticles with PEG increased the efficiency of drug delivery into tumor tissue and subsequently more effective antitumor activity.Conclusion: This specific design of a chemotherapeutic agent using nanotechnology is important in the development of a safe and effective drug in cancer therapy.Keywords: lipid nanoparticles, gold porphyrin, cancer, neuroblastoma, camptothecin
format	article
author	Lee P Zhang R Li V Liu X Sun RWY Che CM Wong KKY
author_facet	Lee P Zhang R Li V Liu X Sun RWY Che CM Wong KKY
author_sort	Lee P
title	Enhancement of anticancer efficacy using modified lipophilic nanoparticle drug encapsulation
title_short	Enhancement of anticancer efficacy using modified lipophilic nanoparticle drug encapsulation
title_full	Enhancement of anticancer efficacy using modified lipophilic nanoparticle drug encapsulation
title_fullStr	Enhancement of anticancer efficacy using modified lipophilic nanoparticle drug encapsulation
title_full_unstemmed	Enhancement of anticancer efficacy using modified lipophilic nanoparticle drug encapsulation
title_sort	enhancement of anticancer efficacy using modified lipophilic nanoparticle drug encapsulation
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/d657e2769eae463db493c73c63cc4a9d
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_version_	1718399546861551616

Enhancement of anticancer efficacy using modified lipophilic nanoparticle drug encapsulation

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