Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase

The bacterial helicase-like transcription factor HelD associates with the RNA polymerase (RNAP) and recycles stalled transcription complexes. Here, the authors present the cryo-EM structures of three Mycobacterium smegmatis HelD bound RNAP complexes and further show that HelD can prevent the binding...

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Autores principales:	Tomáš Kouba, Tomáš Koval’, Petra Sudzinová, Jiří Pospíšil, Barbora Brezovská, Jarmila Hnilicová, Hana Šanderová, Martina Janoušková, Michaela Šiková, Petr Halada, Michal Sýkora, Ivan Barvík, Jiří Nováček, Mária Trundová, Jarmila Dušková, Tereza Skálová, URee Chon, Katsuhiko S. Murakami, Jan Dohnálek, Libor Krásný
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/d65a4f9aaa6b499b97c7834c0fbc8302
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spelling	oai:doaj.org-article:d65a4f9aaa6b499b97c7834c0fbc83022021-12-02T13:57:45ZMycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase10.1038/s41467-020-20158-42041-1723https://doaj.org/article/d65a4f9aaa6b499b97c7834c0fbc83022020-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-20158-4https://doaj.org/toc/2041-1723The bacterial helicase-like transcription factor HelD associates with the RNA polymerase (RNAP) and recycles stalled transcription complexes. Here, the authors present the cryo-EM structures of three Mycobacterium smegmatis HelD bound RNAP complexes and further show that HelD can prevent the binding of the RNAP core to non-specific DNA and also actively removes RNAP from stalled elongation complexes.Tomáš KoubaTomáš Koval’Petra SudzinováJiří PospíšilBarbora BrezovskáJarmila HnilicováHana ŠanderováMartina JanouškováMichaela ŠikováPetr HaladaMichal SýkoraIvan BarvíkJiří NováčekMária TrundováJarmila DuškováTereza SkálováURee ChonKatsuhiko S. MurakamiJan DohnálekLibor KrásnýNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Tomáš Kouba Tomáš Koval’ Petra Sudzinová Jiří Pospíšil Barbora Brezovská Jarmila Hnilicová Hana Šanderová Martina Janoušková Michaela Šiková Petr Halada Michal Sýkora Ivan Barvík Jiří Nováček Mária Trundová Jarmila Dušková Tereza Skálová URee Chon Katsuhiko S. Murakami Jan Dohnálek Libor Krásný Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase
description	The bacterial helicase-like transcription factor HelD associates with the RNA polymerase (RNAP) and recycles stalled transcription complexes. Here, the authors present the cryo-EM structures of three Mycobacterium smegmatis HelD bound RNAP complexes and further show that HelD can prevent the binding of the RNAP core to non-specific DNA and also actively removes RNAP from stalled elongation complexes.
format	article
author	Tomáš Kouba Tomáš Koval’ Petra Sudzinová Jiří Pospíšil Barbora Brezovská Jarmila Hnilicová Hana Šanderová Martina Janoušková Michaela Šiková Petr Halada Michal Sýkora Ivan Barvík Jiří Nováček Mária Trundová Jarmila Dušková Tereza Skálová URee Chon Katsuhiko S. Murakami Jan Dohnálek Libor Krásný
author_facet	Tomáš Kouba Tomáš Koval’ Petra Sudzinová Jiří Pospíšil Barbora Brezovská Jarmila Hnilicová Hana Šanderová Martina Janoušková Michaela Šiková Petr Halada Michal Sýkora Ivan Barvík Jiří Nováček Mária Trundová Jarmila Dušková Tereza Skálová URee Chon Katsuhiko S. Murakami Jan Dohnálek Libor Krásný
author_sort	Tomáš Kouba
title	Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase
title_short	Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase
title_full	Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase
title_fullStr	Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase
title_full_unstemmed	Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase
title_sort	mycobacterial held is a nucleic acids-clearing factor for rna polymerase
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/d65a4f9aaa6b499b97c7834c0fbc8302
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Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase

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