Molecular Characterization of Associated Pathogens in Febrile Patients during Inter-Epidemic Periods of Urban Arboviral Diseases in Tapachula Southern Mexico

Emerging and re-emerging vector-borne infections are a global public health threat. In endemic regions, fever is the main reason for medical attention, and the etiological agent of such fever is not usually identified. In this study, non-specific febrile pathogens were molecularly characterized in s...

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Autores principales:	Geovana Calvo-Anguiano, José de Jesús Lugo-Trampe, Gustavo Ponce-García, Angel Lugo-Trampe, Laura Elia Martinez-Garza, Marisol Ibarra-Ramirez, Luis Daniel Campos-Acevedo, Sandra Caballero-Sosa, Alan Esteban Juache-Villagrana, Ildefonso Fernández-Salas, Adriana Elizabeth Flores-Suarez, Iram Pablo Rodriguez-Sanchez, Karina del Carmen Trujillo-Murillo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Arbovirus Chikungunya ZIKA Leptospira Flavivirus Alphavirus Medicine R
Acceso en línea:	https://doaj.org/article/d65aad5d3a484c45a261d044c1155819
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spelling	oai:doaj.org-article:d65aad5d3a484c45a261d044c11558192021-11-25T18:38:21ZMolecular Characterization of Associated Pathogens in Febrile Patients during Inter-Epidemic Periods of Urban Arboviral Diseases in Tapachula Southern Mexico10.3390/pathogens101114502076-0817https://doaj.org/article/d65aad5d3a484c45a261d044c11558192021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1450https://doaj.org/toc/2076-0817Emerging and re-emerging vector-borne infections are a global public health threat. In endemic regions, fever is the main reason for medical attention, and the etiological agent of such fever is not usually identified. In this study, non-specific febrile pathogens were molecularly characterized in serum samples from 253 patients suspected of arbovirus infection. The samples were collected in the southern border region of Mexico from April to June 2015, and February to March 2016. ZIKV, CHIKV, DENV, leptospirosis, and rickettsiosis were detected by qPCR and nested PCR to identify flavivirus and alphavirus genera. The results indicated that 71.93% of the samples were positive for CHIKV, 0.79% for ZIKV, and 0.39% for DENV, with the number positive for CHIKV increasing to 76.67% and those positive for ZIKV increasing to 15.41% under the nested PCR technique. <i>Leptospira Kmetyi</i> was identified for the first time in Mexico, with a prevalence of 3.16%. This is the first report of ZIKV in Mexico, as well the first detection of the virus in early 2015. In conclusion, the etiological agent of fever was determined in 94% of the analyzed samples.Geovana Calvo-AnguianoJosé de Jesús Lugo-TrampeGustavo Ponce-GarcíaAngel Lugo-TrampeLaura Elia Martinez-GarzaMarisol Ibarra-RamirezLuis Daniel Campos-AcevedoSandra Caballero-SosaAlan Esteban Juache-VillagranaIldefonso Fernández-SalasAdriana Elizabeth Flores-SuarezIram Pablo Rodriguez-SanchezKarina del Carmen Trujillo-MurilloMDPI AGarticleArbovirusChikungunyaZIKALeptospiraFlavivirusAlphavirusMedicineRENPathogens, Vol 10, Iss 1450, p 1450 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arbovirus Chikungunya ZIKA Leptospira Flavivirus Alphavirus Medicine R
spellingShingle	Arbovirus Chikungunya ZIKA Leptospira Flavivirus Alphavirus Medicine R Geovana Calvo-Anguiano José de Jesús Lugo-Trampe Gustavo Ponce-García Angel Lugo-Trampe Laura Elia Martinez-Garza Marisol Ibarra-Ramirez Luis Daniel Campos-Acevedo Sandra Caballero-Sosa Alan Esteban Juache-Villagrana Ildefonso Fernández-Salas Adriana Elizabeth Flores-Suarez Iram Pablo Rodriguez-Sanchez Karina del Carmen Trujillo-Murillo Molecular Characterization of Associated Pathogens in Febrile Patients during Inter-Epidemic Periods of Urban Arboviral Diseases in Tapachula Southern Mexico
description	Emerging and re-emerging vector-borne infections are a global public health threat. In endemic regions, fever is the main reason for medical attention, and the etiological agent of such fever is not usually identified. In this study, non-specific febrile pathogens were molecularly characterized in serum samples from 253 patients suspected of arbovirus infection. The samples were collected in the southern border region of Mexico from April to June 2015, and February to March 2016. ZIKV, CHIKV, DENV, leptospirosis, and rickettsiosis were detected by qPCR and nested PCR to identify flavivirus and alphavirus genera. The results indicated that 71.93% of the samples were positive for CHIKV, 0.79% for ZIKV, and 0.39% for DENV, with the number positive for CHIKV increasing to 76.67% and those positive for ZIKV increasing to 15.41% under the nested PCR technique. <i>Leptospira Kmetyi</i> was identified for the first time in Mexico, with a prevalence of 3.16%. This is the first report of ZIKV in Mexico, as well the first detection of the virus in early 2015. In conclusion, the etiological agent of fever was determined in 94% of the analyzed samples.
format	article
author	Geovana Calvo-Anguiano José de Jesús Lugo-Trampe Gustavo Ponce-García Angel Lugo-Trampe Laura Elia Martinez-Garza Marisol Ibarra-Ramirez Luis Daniel Campos-Acevedo Sandra Caballero-Sosa Alan Esteban Juache-Villagrana Ildefonso Fernández-Salas Adriana Elizabeth Flores-Suarez Iram Pablo Rodriguez-Sanchez Karina del Carmen Trujillo-Murillo
author_facet	Geovana Calvo-Anguiano José de Jesús Lugo-Trampe Gustavo Ponce-García Angel Lugo-Trampe Laura Elia Martinez-Garza Marisol Ibarra-Ramirez Luis Daniel Campos-Acevedo Sandra Caballero-Sosa Alan Esteban Juache-Villagrana Ildefonso Fernández-Salas Adriana Elizabeth Flores-Suarez Iram Pablo Rodriguez-Sanchez Karina del Carmen Trujillo-Murillo
author_sort	Geovana Calvo-Anguiano
title	Molecular Characterization of Associated Pathogens in Febrile Patients during Inter-Epidemic Periods of Urban Arboviral Diseases in Tapachula Southern Mexico
title_short	Molecular Characterization of Associated Pathogens in Febrile Patients during Inter-Epidemic Periods of Urban Arboviral Diseases in Tapachula Southern Mexico
title_full	Molecular Characterization of Associated Pathogens in Febrile Patients during Inter-Epidemic Periods of Urban Arboviral Diseases in Tapachula Southern Mexico
title_fullStr	Molecular Characterization of Associated Pathogens in Febrile Patients during Inter-Epidemic Periods of Urban Arboviral Diseases in Tapachula Southern Mexico
title_full_unstemmed	Molecular Characterization of Associated Pathogens in Febrile Patients during Inter-Epidemic Periods of Urban Arboviral Diseases in Tapachula Southern Mexico
title_sort	molecular characterization of associated pathogens in febrile patients during inter-epidemic periods of urban arboviral diseases in tapachula southern mexico
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d65aad5d3a484c45a261d044c1155819
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Molecular Characterization of Associated Pathogens in Febrile Patients during Inter-Epidemic Periods of Urban Arboviral Diseases in Tapachula Southern Mexico

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