Genetic variability of Begonia longifolia Blume from Indonesia based on Nuclear DNA internal transcribed spacer (ITS) sequence data

Abstract.Ningrum WDA, Atmaja MB, Daryono BS, Purnomo. 2020. Genetic variability of Begonia longifolia Blume from Indonesia based on Nuclear DNA internal transcribed spacer (ITS) sequence data. Biodiversitas 21: 5778-5785. Begonia longifolia Blume is a member of the Begoniaceae family which has the w...

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Autores principales:	Wahyu Dewi Astuti Ningrum, Muhammad Bima Atmaja, Budi Setiadi Daryono, Purnomo PURNOMO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	begonia longifolia, intraspecific, molecular, nr dna its, phylogenetic Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d65fd6d0e50d449bbd3bcefbdbd04ccb
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spelling	oai:doaj.org-article:d65fd6d0e50d449bbd3bcefbdbd04ccb2021-11-22T00:52:01ZGenetic variability of Begonia longifolia Blume from Indonesia based on Nuclear DNA internal transcribed spacer (ITS) sequence data1412-033X2085-472210.13057/biodiv/d211239https://doaj.org/article/d65fd6d0e50d449bbd3bcefbdbd04ccb2020-11-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6785https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract.Ningrum WDA, Atmaja MB, Daryono BS, Purnomo. 2020. Genetic variability of Begonia longifolia Blume from Indonesia based on Nuclear DNA internal transcribed spacer (ITS) sequence data. Biodiversitas 21: 5778-5785. Begonia longifolia Blume is a member of the Begoniaceae family which has the widest distribution in Asia including Indonesia. Diversity studies on B.longifolia from Indonesia were mainly based on morphological characters. The genetic diversity analysis of this pantropical species in Indonesia has never been reported yet. This research aimed to study genetic variability of B. longifolia in Indonesia based on nuclear DNA internal transcribed spacer (ITS) sequence data. Twelve living specimens were from Sumatra (Lampung), Java (Sukabumi, Purwokerto), Bali (Buleleng, Gianyar, Jembrana, Tabanan), and Lombok. The 831-839 length sequence was aligned using Mesquite software. Reconstruction of the phylogenetic tree was done by MEGA7 software using Neighbor-Joining method with Kimura-2-Parameter. The 827 fixed sites showed a genetic distance ranged from 0 to 1.6%. Among the 827 sites compared, 802 sites were invariable, 19 sites were variable (11 singleton variable sites and 8 parsimony informative sites) and 6 sites were gaps. The phylogenetic tree showed 1 clade and 6 sub-clades. Specimens from Sumatra and Java formed a single sub-clade, separate with five sub-clades from Bali. Specimen from Lombok belong to one of the sub-clades of the Bali specimens. All specimens can be divided into 2 groups: Sumatra-Java and Bali-Lombok group.Wahyu Dewi Astuti NingrumMuhammad Bima AtmajaBudi Setiadi DaryonoPurnomo PURNOMOMBI & UNS Soloarticlebegonia longifolia, intraspecific, molecular, nr dna its, phylogeneticBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	begonia longifolia, intraspecific, molecular, nr dna its, phylogenetic Biology (General) QH301-705.5
spellingShingle	begonia longifolia, intraspecific, molecular, nr dna its, phylogenetic Biology (General) QH301-705.5 Wahyu Dewi Astuti Ningrum Muhammad Bima Atmaja Budi Setiadi Daryono Purnomo PURNOMO Genetic variability of Begonia longifolia Blume from Indonesia based on Nuclear DNA internal transcribed spacer (ITS) sequence data
description	Abstract.Ningrum WDA, Atmaja MB, Daryono BS, Purnomo. 2020. Genetic variability of Begonia longifolia Blume from Indonesia based on Nuclear DNA internal transcribed spacer (ITS) sequence data. Biodiversitas 21: 5778-5785. Begonia longifolia Blume is a member of the Begoniaceae family which has the widest distribution in Asia including Indonesia. Diversity studies on B.longifolia from Indonesia were mainly based on morphological characters. The genetic diversity analysis of this pantropical species in Indonesia has never been reported yet. This research aimed to study genetic variability of B. longifolia in Indonesia based on nuclear DNA internal transcribed spacer (ITS) sequence data. Twelve living specimens were from Sumatra (Lampung), Java (Sukabumi, Purwokerto), Bali (Buleleng, Gianyar, Jembrana, Tabanan), and Lombok. The 831-839 length sequence was aligned using Mesquite software. Reconstruction of the phylogenetic tree was done by MEGA7 software using Neighbor-Joining method with Kimura-2-Parameter. The 827 fixed sites showed a genetic distance ranged from 0 to 1.6%. Among the 827 sites compared, 802 sites were invariable, 19 sites were variable (11 singleton variable sites and 8 parsimony informative sites) and 6 sites were gaps. The phylogenetic tree showed 1 clade and 6 sub-clades. Specimens from Sumatra and Java formed a single sub-clade, separate with five sub-clades from Bali. Specimen from Lombok belong to one of the sub-clades of the Bali specimens. All specimens can be divided into 2 groups: Sumatra-Java and Bali-Lombok group.
format	article
author	Wahyu Dewi Astuti Ningrum Muhammad Bima Atmaja Budi Setiadi Daryono Purnomo PURNOMO
author_facet	Wahyu Dewi Astuti Ningrum Muhammad Bima Atmaja Budi Setiadi Daryono Purnomo PURNOMO
author_sort	Wahyu Dewi Astuti Ningrum
title	Genetic variability of Begonia longifolia Blume from Indonesia based on Nuclear DNA internal transcribed spacer (ITS) sequence data
title_short	Genetic variability of Begonia longifolia Blume from Indonesia based on Nuclear DNA internal transcribed spacer (ITS) sequence data
title_full	Genetic variability of Begonia longifolia Blume from Indonesia based on Nuclear DNA internal transcribed spacer (ITS) sequence data
title_fullStr	Genetic variability of Begonia longifolia Blume from Indonesia based on Nuclear DNA internal transcribed spacer (ITS) sequence data
title_full_unstemmed	Genetic variability of Begonia longifolia Blume from Indonesia based on Nuclear DNA internal transcribed spacer (ITS) sequence data
title_sort	genetic variability of begonia longifolia blume from indonesia based on nuclear dna internal transcribed spacer (its) sequence data
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/d65fd6d0e50d449bbd3bcefbdbd04ccb
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Genetic variability of Begonia longifolia Blume from Indonesia based on Nuclear DNA internal transcribed spacer (ITS) sequence data

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