Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis

Sulistyaningsih LD, Abinawanto, Ardiyani M, Salamah A. 2018. Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis. Biodiversitas 19: 364-368. Smilax spp. (Smilacaceae) has long been used as medicinal herbs e...

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Autores principales:	LULUT DWI SULISTYANINGSIH, MARLINA ARDIYANI, ABINAWANTO ABINAWANTO, ANDI SALAMAH
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	dna barcode java smilax rbcl Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d65ff29918e143a487d71e0155c6a658
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spelling	oai:doaj.org-article:d65ff29918e143a487d71e0155c6a6582021-11-16T14:01:41ZShort Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis1412-033X2085-472210.13057/biodiv/d190202https://doaj.org/article/d65ff29918e143a487d71e0155c6a6582018-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2459https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Sulistyaningsih LD, Abinawanto, Ardiyani M, Salamah A. 2018. Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis. Biodiversitas 19: 364-368. Smilax spp. (Smilacaceae) has long been used as medicinal herbs especially in East Asia and North America as they were known to be rich in steroidal saponin. Pharmacological study has been carried out in Indonesia. This genus is widespread in Indonesia and fairly abundant in Java and has been known either as edible fruit or medicinal plants. Characteristics of Smilax as a dioecious plant with high morphological variations make it thorny in species identification. Various molecular approaches have been devised to overcome identification problems such as DNA barcoding. This study, therefore was conducted to analyze the DNA barcoding application for phylogenetic and identification of Smilax in Java. A total of 31 samples were used in this study including 19 accession numbers from NCBI GeneBank. The genus Ripogonum was used as the out-group in phylogenetic reconstruction. Samples were successfully extracted by CTAB method with some modifications. rbcL region was used as the DNA barcode showed sufficient variation and conserved flanks. Two unidentified specimens have high similarity with S. leucophyla and lies in the same clade. The phylogenetic tree constructed by Maximum Likelihood analysis. The result showed that the monophyletic of Smilacaceae consisted of four clades. The genus Heterosmilax nested with Smilax though with low bootstraps value. It supports the monogeneric status of Smilacaceae.LULUT DWI SULISTYANINGSIHMARLINA ARDIYANIABINAWANTO ABINAWANTOANDI SALAMAHMBI & UNS Soloarticledna barcodejavasmilaxrbclBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 2, Pp 364-368 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	dna barcode java smilax rbcl Biology (General) QH301-705.5
spellingShingle	dna barcode java smilax rbcl Biology (General) QH301-705.5 LULUT DWI SULISTYANINGSIH MARLINA ARDIYANI ABINAWANTO ABINAWANTO ANDI SALAMAH Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis
description	Sulistyaningsih LD, Abinawanto, Ardiyani M, Salamah A. 2018. Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis. Biodiversitas 19: 364-368. Smilax spp. (Smilacaceae) has long been used as medicinal herbs especially in East Asia and North America as they were known to be rich in steroidal saponin. Pharmacological study has been carried out in Indonesia. This genus is widespread in Indonesia and fairly abundant in Java and has been known either as edible fruit or medicinal plants. Characteristics of Smilax as a dioecious plant with high morphological variations make it thorny in species identification. Various molecular approaches have been devised to overcome identification problems such as DNA barcoding. This study, therefore was conducted to analyze the DNA barcoding application for phylogenetic and identification of Smilax in Java. A total of 31 samples were used in this study including 19 accession numbers from NCBI GeneBank. The genus Ripogonum was used as the out-group in phylogenetic reconstruction. Samples were successfully extracted by CTAB method with some modifications. rbcL region was used as the DNA barcode showed sufficient variation and conserved flanks. Two unidentified specimens have high similarity with S. leucophyla and lies in the same clade. The phylogenetic tree constructed by Maximum Likelihood analysis. The result showed that the monophyletic of Smilacaceae consisted of four clades. The genus Heterosmilax nested with Smilax though with low bootstraps value. It supports the monogeneric status of Smilacaceae.
format	article
author	LULUT DWI SULISTYANINGSIH MARLINA ARDIYANI ABINAWANTO ABINAWANTO ANDI SALAMAH
author_facet	LULUT DWI SULISTYANINGSIH MARLINA ARDIYANI ABINAWANTO ABINAWANTO ANDI SALAMAH
author_sort	LULUT DWI SULISTYANINGSIH
title	Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis
title_short	Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis
title_full	Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis
title_fullStr	Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis
title_full_unstemmed	Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis
title_sort	short communication: phylogenetic analysis and molecular identification of canar (smilax spp.) in java, indonesia based on dna barcoding analysis
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/d65ff29918e143a487d71e0155c6a658
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Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis

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